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However, the nuclear localization of PARP imposes a stringent requirement on probe design, as fluorophore installation must not compromise nuclear entry of the targeting ligand. Herein, we report a focused series of 5\u2010azetidinyl BODIPY\u2013olaparib conjugates as PARP\u2010targeted fluorescent probes. By attaching olaparib\u2010derived ligands to the 5\u2010position of the BODIPY core through flexible linkers, we sought to preserve conformational flexibility while introducing a donor\u2013acceptor fluorophore with an enlarged Stokes shift. Among the synthesized probes,\n                    <jats:bold>1a<\/jats:bold>\n                    exhibited the most favorable cellular imaging performance, showing efficient nuclear localization in PARP\u2010overexpressing U87 and Cal27 cells. Competitive blocking with olaparib and comparison with HEK293 cells supported PARP\u2010dependent imaging. In Cal27 xenograft and orthotopic oral tumor models,\n                    <jats:bold>1a<\/jats:bold>\n                    showed rapid tumor accumulation and clear tumor visualization. It provides a wider range of probe options for intraoperative tracking of oral tumors.\n                  <\/jats:p>","DOI":"10.1002\/cmdc.70450","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T04:25:39Z","timestamp":1787199939000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A 5\u2010Azetidinyl BODIPY\u2013Olaparib Conjugate for PARP\u2010Targeted Nuclear and Orthotopic Oral Tumor Imaging"],"prefix":"10.1002","volume":"21","author":[{"given":"Kaixiang","family":"Yan","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences Fudan University  Shanghai P. R. 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