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The designed compounds were synthesized via a multistep synthetic route comprising phenylhydrazone formation, Fischer indole cyclization, Vilsmeier\u2013Haack formylation, and subsequent Schiff base condensation. Structural elucidation was performed using IR,\n                    <jats:sup>1<\/jats:sup>\n                    H NMR,\n                    <jats:sup>13<\/jats:sup>\n                    C NMR, and mass spectral analysis. In silico studies demonstrated that the synthesized compounds exhibited strong binding affinities to both wild\u2010type (PDB ID: 1M17) and mutant EGFR (PDB ID: 4ZAU), as evidenced by favorable docking scores, with PYd2 showing the strongest interaction. Molecular dynamics simulations over 100\u2009ns further confirmed that the PYd2\u2013EGFR complexes remained stable, maintaining consistent protein\u2013ligand contacts and exhibiting stable RMSD and RMSF values throughout the simulation period. Predicted ADME\u2010toxicity profiles demonstrated favorable drug\u2010like properties, oral absorption, and acceptable toxicity profiles for most synthesized derivatives. In vitro anticancer evaluation against MDA\u2010MB\u2010231 and SK\u2010OV\u20103 cell lines identified PYd2 as the most potent analog. ELISA\u2010based studies demonstrated significant inhibition of EGFR expression and phosphorylation, while DAPI staining revealed characteristic apoptotic nuclear changes in treated cells. Overall, PYd2 showed favorable predicted EGFR binding and antiproliferative activity against both cancer cell lines.\n                  <\/jats:p>","DOI":"10.1002\/cmdc.70541","type":"journal-article","created":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T04:21:23Z","timestamp":1792470083000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Design, Synthesis, and Biological Evaluation of Quinolin\u20102\u2010One\u2013Indole Schiff Base Hybrids as Potential EGFR\u2010Targeted Anticancer Agents Against Breast and Ovarian Cancer"],"prefix":"10.1002","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4551-9247","authenticated-orcid":false,"given":"Prachita Gauns","family":"Dessai","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Chemistry KLE College of Pharmacy  Belagavi India"},{"name":"Department of Pharmaceutical Chemistry P.E.S\u2019s Rajaram and Tarabai Bandekar College of Pharmacy  Goa India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3020-0113","authenticated-orcid":false,"given":"Parixit","family":"Bhandurge","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry KLE College of Pharmacy  Belagavi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8534-6462","authenticated-orcid":false,"given":"Celina","family":"Nazareth","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry P.E.S\u2019s Rajaram and Tarabai Bandekar College of Pharmacy  Goa India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5388-1663","authenticated-orcid":false,"given":"Sahili","family":"Naik","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry KLE College of Pharmacy  Belagavi India"},{"name":"Department of Pharmaceutical Chemistry P.E.S\u2019s Rajaram and Tarabai Bandekar College of Pharmacy  Goa India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,19]]},"reference":[{"key":"e_1_2_10_2_1","unstructured":"Data Visualization Tools for Exploring the Global Cancer Burden in 2022 2024 accessed August 5 \u00a02026 https:\/\/gco.iarc.who.int\/today\/en."},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21834"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.2174\/1568026620666200303123102"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers9050052"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells13010047"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/14728222.2023.2218613"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.2174\/0115680266314303240830074056"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2005.12.017"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2007.08.009"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436\u2010012\u20102886\u2010x"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2006.02.039"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm020241c"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2018.10.038"},{"key":"e_1_2_10_15_1","first-page":"1","volume-title":"Protein Kinase Inhibitors as Sensitizing Agents for Chemotherapy","author":"Barbuti A. 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