{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T16:45:36Z","timestamp":1761237936382},"reference-count":41,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,9,18]],"date-time":"2012-09-18T00:00:00Z","timestamp":1347926400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Contrast Media &amp; Molecular"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:p>Capitalizing on cellular homing to cancer is a promising strategy for targeting malignant cells for diagnostic, monitoring and therapeutic purposes. Murine C17.2 neural progenitor cells (NPC) demonstrate a tropism for cell line\u2010derived tumors, but their affinity for patient\u2010derived tumors is unknown. We tested the hypothesis that NPC accumulate in patient\u2010derived tumors at levels detectable by optical imaging. Mice bearing solid tumors after transplantation with patient\u2010derived leukemia cells and untransplanted controls received 10<jats:sup>6<\/jats:sup> fluorescent DiR\u2010labeled NPC daily for 1\u20134 days, were imaged, then sacrificed. Tissues were analyzed by immunofluorescence and flow cytometry to detect tumor cell engraftment (CD45) and NPC (FITC\u2010<jats:italic>\u03b2<\/jats:italic> galactosidase or DiR). Tumors consisted primarily of CD45\u2010positive cells and demonstrated mild fluorescence, corresponding to frequent clusters of FITC\u2010<jats:italic>\u03b2<\/jats:italic> gal\u2010positive cells. Both transplanted and control mice demonstrated the highest fluorescent signal in the spleens and other tissues of the reticuloendothelial activating system. However, only rare FITC\u2010<jats:italic>\u03b2<\/jats:italic> gal\u2010positive cells were detected in the mildly engrafted transplanted spleens and none in the control spleens, suggesting that their high DiR signal reflects the sequestration of DiR\u2010positive debris. The mildly engrafted transplanted kidneys demonstrated low fluorescent signal and rare FITC\u2010<jats:italic>\u03b2<\/jats:italic> gal\u2010positive cells whereas control kidneys were negative. Results indicate that NPC accumulate in tissues containing patient\u2010derived tumor cells in a manner that is detectable by <jats:italic>ex vivo<\/jats:italic> optical imaging and proportional to the level of tumor engraftment, suggesting a capacity to home to micrometastatic disease. As such, NPC could have significant clinical applications for the targeted diagnosis and treatment of cancer. Copyright \u00a9 2012 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/cmmi.1485","type":"journal-article","created":{"date-parts":[[2012,9,19]],"date-time":"2012-09-19T03:01:56Z","timestamp":1348023716000},"page":"525-536","source":"Crossref","is-referenced-by-count":2,"title":["Optical imaging of progenitor cell homing to patient\u2010derived tumors"],"prefix":"10.1002","volume":"7","author":[{"given":"Isabel G.","family":"Newton","sequence":"first","affiliation":[{"name":"University of California  San Diego CA 92103\u20108756 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Warren C.","family":"Plaisted","sequence":"additional","affiliation":[{"name":"University of California  San Diego CA 92103\u20108756 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven","family":"Messina\u2010Graham","sequence":"additional","affiliation":[{"name":"University of California  San Diego CA 92103\u20108756 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annelie E.","family":"Abrahamsson Schairer","sequence":"additional","affiliation":[{"name":"University of California  San Diego CA 92103\u20108756 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alice Y.","family":"Shih","sequence":"additional","affiliation":[{"name":"University of California  San Diego CA 92103\u20108756 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evan Y.","family":"Snyder","sequence":"additional","affiliation":[{"name":"University of California  San Diego CA 92103\u20108756 USA"},{"name":"Sanford-Burnham Medical Research Institute  10901 North Torrey Pines Road La Jolla CA 92037 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catriona H. 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