{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T22:47:29Z","timestamp":1791154049849,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,4,14]],"date-time":"2014-04-14T00:00:00Z","timestamp":1397433600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Contrast Media &amp; Molecular"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:p>There is an increasing interest in developing novel imaging strategies for sensing proteolytic activities in intact organisms <jats:italic>in vivo<\/jats:italic>. Overhauser\u2010enhanced MRI (OMRI) offers the possibility to reveal the proteolysis of nitroxide\u2010labeled macromolecules thanks to a sharp decrease of the rotational correlation time of the nitroxide moiety upon cleavage. In this paper, this concept is illustrated <jats:italic>in vivo<\/jats:italic> at 0.2\u2009T using nitroxide\u2010labeled elastin orally administered in mice. <jats:italic>In vitro<\/jats:italic>, this elastin derivative was OMRI\u2010visible and gave rise to high Overhauser enhancements (19\u2010fold at 18\u2009m<jats:sc>m<\/jats:sc> nitroxide) upon proteolysis by pancreatic porcine elastase. <jats:italic>In vivo<\/jats:italic> three\u2010dimensional OMRI detection of proteolysis was carried out. A keyhole fully balanced steady\u2010state free precession sequence was used, which allowed 3D OMRI acquisition within 20\u2009s at 0.125\u2009mm<jats:sup>3<\/jats:sup> resolution. About 30\u2009min after mouse gavage, proteolysis was detected in the duodenum, where Overhauser enhancements were 7.2\u2009\u00b1\u20092.4 (n\u2009=\u20097) and was not observed in the stomach. Conversely, orally administered free nitroxides or pre\u2010digested nitroxide\u2010labeled elastin were detected in the mouse's stomach by OMRI. Combined with specific molecular probes, this Overhauser\u2010enhanced MRI technique can be used to evaluate unregulated proteolytic activities in various models of experimental diseases and for drug testing. Copyright \u00a9 2014 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/cmmi.1586","type":"journal-article","created":{"date-parts":[[2014,4,14]],"date-time":"2014-04-14T07:38:54Z","timestamp":1397461134000},"page":"363-371","source":"Crossref","is-referenced-by-count":39,"title":["<i>In vivo<\/i> Overhauser\u2010enhanced MRI of proteolytic activity"],"prefix":"10.1002","volume":"9","author":[{"given":"Neha","family":"Koonjoo","sequence":"first","affiliation":[{"name":"Centre de R\u00e9sonance Magn\u00e9tique des Syst\u00e8mes Biologiques UMR 5536 CNRS Universit\u00e9 Bordeaux Segalen  Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elodie","family":"Parzy","sequence":"additional","affiliation":[{"name":"Centre de R\u00e9sonance Magn\u00e9tique des Syst\u00e8mes Biologiques UMR 5536 CNRS Universit\u00e9 Bordeaux Segalen  Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Massot","sequence":"additional","affiliation":[{"name":"Centre de R\u00e9sonance Magn\u00e9tique des Syst\u00e8mes Biologiques UMR 5536 CNRS Universit\u00e9 Bordeaux Segalen  Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthieu","family":"Lepetit\u2010Coiff\u00e9","sequence":"additional","affiliation":[{"name":"Centre de R\u00e9sonance Magn\u00e9tique des Syst\u00e8mes Biologiques UMR 5536 CNRS Universit\u00e9 Bordeaux Segalen  Bordeaux France"},{"name":"Siemens France  Saint\u2010Denis France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvain R. 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