{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T02:30:01Z","timestamp":1790649001287,"version":"4.1.0"},"reference-count":76,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,10,18]],"date-time":"2014-10-18T00:00:00Z","timestamp":1413590400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003968","name":"Iran National Science foundation","doi-asserted-by":"crossref","award":["92013079"],"award-info":[{"award-number":["92013079"]}],"id":[{"id":"10.13039\/501100003968","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Contrast Media &amp; Molecular"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:p>\n                    MUC1 antigen is recognized as a high\u2010molecular\u2010weight glycoprotein that is unexpectedly over\u2010expressed in human breast and other carcinomas. In contrast, C595 a monoclonal antibody (mAb) against the protein core of the human urinary epithelial machine, is commonly expressed in breast carcinomas. The aim of this study was to conjugate ultra\u2010small super paramagnetic iron oxide nanoparticles (USPIO) with C595 mAb, in order to detect\n                    <jats:italic>in vivo<\/jats:italic>\n                    MUC1 expression. A dual contrast agent (the C595 antibody\u2010conjugated USPIO labeled with 99mTc) was prepared for targeted imaging and therapy of anti\u2010MUC1\u2010expressing cancers. The C595 antibody\u2010conjugated USPIO had good stability and reactivity in the presence of blood plasma at 37\u2009\u00b0C. No significant differences were observed in immunoreactivity results between conjugated and nonconjugated nanoparticles. The\n                    <jats:italic>T<\/jats:italic>\n                    <jats:sub>1<\/jats:sub>\n                    and\n                    <jats:italic>T<\/jats:italic>\n                    <jats:sub>2<\/jats:sub>\n                    measurements show &gt;79 and 29% increments (for 0.02\u2009mg\/ml iron concentrations) in\n                    <jats:italic>T<\/jats:italic>\n                    <jats:sub>1<\/jats:sub>\n                    and\n                    <jats:italic>T<\/jats:italic>\n                    <jats:sub>2<\/jats:sub>\n                    values for USPIO\u2010C595 in comparison with USPIO, respectively. The nanoprobes showed the interesting targeting capability of finding the MUC1\u2010positive cell line\n                    <jats:italic>in vitro<\/jats:italic>\n                    . However, we found disappointing\n                    <jats:italic>in vivo<\/jats:italic>\n                    results (i.e. very low accumulation of nanoprobes in the targeted site while &gt;80% of the injected dose per gram was taken up by the liver and spleen), not only due to the coverage of targeting site by protein corona but also because of absorption of opsonin\u2010based proteins at the surface of nanoprobes. Copyright \u00a9 2014 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/cmmi.1627","type":"journal-article","created":{"date-parts":[[2014,10,18]],"date-time":"2014-10-18T08:04:58Z","timestamp":1413619498000},"page":"225-236","source":"Crossref","is-referenced-by-count":47,"title":["Monoclonal antibody conjugated magnetic nanoparticles could target MUC\u20101\u2010positive cells\n                    <i>in vitro<\/i>\n                    but not\n                    <i>in vivo<\/i>"],"prefix":"10.1002","volume":"10","author":[{"given":"Saeed","family":"Shanehsazzadeh","sequence":"first","affiliation":[{"name":"Radiation Application Research School Nuclear Science and Technology Research Institute (NSTRI)  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cordula","family":"Gruettner","sequence":"additional","affiliation":[{"name":"Micromod Partikeltechnologie GmbH Friedrich\u2010Barnewitz\u2010Str. 4  D\u201018119 Rostock Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Afsaneh","family":"Lahooti","sequence":"additional","affiliation":[{"name":"Department of Medical Physics and Biomedical Engineering Faculty of Medicine, Tehran University of Medical Sciences  Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morteza","family":"Mahmoudi","sequence":"additional","affiliation":[{"name":"Nanotechnology Research Center and Department of Nanotechnology Faculty of Pharmacy, Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barry J.","family":"Allen","sequence":"additional","affiliation":[{"name":"Experimental Radiation Oncology, School of Medicine University of Western Sydney  NSW 2217 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahdi","family":"Ghavami","sequence":"additional","affiliation":[{"name":"National cell bank Pasteur Institute of Iran  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fariba Johari","family":"Daha","sequence":"additional","affiliation":[{"name":"Radiation Application Research School Nuclear Science and Technology Research Institute (NSTRI)  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Ali","family":"Oghabian","sequence":"additional","affiliation":[{"name":"Department of Medical Physics and Biomedical Engineering Faculty of Medicine, Tehran University of Medical Sciences  Iran"},{"name":"Biomolecular imaging analysis group (BIAG), Research Center for Molecular and Cellular Imaging (RCMCI) Tehran University of Medical Sciences  Tehran 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