{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T18:37:46Z","timestamp":1760985466874},"reference-count":29,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,9,14]],"date-time":"2016-09-14T00:00:00Z","timestamp":1473811200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Contrast Media &amp; Molecular"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:p>The assessment of the \u03b2\u2010cell mass in experimental models of diabetes and ultimately in patients is a hallmark to understand the relationship between reduced \u03b2\u2010cell mass\/function and the onset of diabetes. It has been shown before that the GLUT\u20102 transporter is highly expressed in both \u03b2\u2010cells and hepatocytes and that D\u2010mannoheptulose (DMH) has high uptake specificity for the GLUT\u20102 transporter. As 19\u2010fluorine MRI has emerged as a new alternative method for MRI cell tracking because it provides potential non\u2010invasive localization and quantification of labeled cells, the purpose of this project is to validate \u03b2\u2010cell and pancreatic islet imaging by using fluorinated, GLUT\u20102 targeting mannoheptulose derivatives (<jats:sup>19<\/jats:sup>FMH) both <jats:italic>in vivo<\/jats:italic> and <jats:italic>ex vivo<\/jats:italic>. In this study, we confirmed that, similar to DMH, <jats:sup>19<\/jats:sup>FMHs inhibit insulin secretion and increase the blood glucose level in mice temporarily (approximately two hours). We were able to assess the distribution of <jats:sup>19<\/jats:sup>FMHs <jats:italic>in vivo<\/jats:italic> with a temporal resolution of about 20\u2009minutes, which showed a quick removal of <jats:sup>19<\/jats:sup>FMH from the circulation (within two hours). <jats:italic>Ex vivo<\/jats:italic> MR spectroscopy confirmed a preferential uptake of <jats:sup>19<\/jats:sup>FMH in tissue with high expression of the GLUT\u20102 transporter, such as liver, endocrine pancreas and kidney. No indication of further metabolism was found. In summary, <jats:sup>19<\/jats:sup>FMHs are potentially suitable for visualizing and tracking of GLUT\u20102 expressed cells. However, current bottlenecks of this technique related to the quick clearance of the compound and relative low sensitivity of <jats:sup>19<\/jats:sup>F MRI need to be overcome. Copyright \u00a9 2016 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/cmmi.1712","type":"journal-article","created":{"date-parts":[[2016,9,14]],"date-time":"2016-09-14T07:27:11Z","timestamp":1473838031000},"page":"506-513","source":"Crossref","is-referenced-by-count":8,"title":["<i>In vivo<\/i> and <i>ex vivo<\/i> 19\u2010fluorine magnetic resonance imaging and spectroscopy of beta\u2010cells and pancreatic islets using GLUT\u20102 specific contrast agents"],"prefix":"10.1002","volume":"11","author":[{"given":"Sayuan","family":"Liang","sequence":"first","affiliation":[{"name":"Biomedical MRI unit\/ MoSAIC, Department of Imaging and Pathology KU Leuven  Leuven Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karim","family":"Louchami","sequence":"additional","affiliation":[{"name":"Biomedical MRI unit\/ MoSAIC, Department of Imaging and Pathology KU Leuven  Leuven Belgium"},{"name":"Laboratory of Experimental Hormonology Universit\u00e9 Libre de Bruxelles  Bruxelles Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hauke","family":"Kolster","sequence":"additional","affiliation":[{"name":"Biomedical MRI unit\/ MoSAIC, Department of Imaging and Pathology KU Leuven  Leuven Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Jacobsen","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science University of Hamburg  Hamburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Hormonology Universit\u00e9 Libre de Bruxelles  Bruxelles Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julian","family":"Thimm","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science University of Hamburg  Hamburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdullah","family":"Sener","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Hormonology Universit\u00e9 Libre de Bruxelles  Bruxelles Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joachim","family":"Thiem","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science University of Hamburg  Hamburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Willy","family":"Malaisse","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Hormonology Universit\u00e9 Libre de Bruxelles  Bruxelles Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tom","family":"Dresselaers","sequence":"additional","affiliation":[{"name":"Biomedical MRI unit\/ MoSAIC, Department of Imaging and Pathology KU Leuven  Leuven Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uwe","family":"Himmelreich","sequence":"additional","affiliation":[{"name":"Biomedical MRI unit\/ MoSAIC, Department of Imaging and Pathology KU Leuven  Leuven Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,9,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.2337\/diacare.27.5.1047"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/dme.12159"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.53.1.250"},{"key":"e_1_2_7_5_1","unstructured":"RodenM.Clinical diabetes research: methods and techniques.Chichester; Hoboken NJ:John Wiley & Sons;2007.xiii 408"},{"key":"e_1_2_7_6_1","volume-title":"Type 1 Diabetes \u2013","author":"Ahlgren U","year":"2011"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2009.03.020"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/13645700801969873"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2010.04.002"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1121"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2012.08.048"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.21352"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s001250051635"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/ejoc.201101238"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/ol2012764"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2011.11.014"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00330-014-3474-5"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0029040"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00592-003-0100-4"},{"issue":"3","key":"e_1_2_7_20_1","first-page":"629","article-title":"el\u2010Naggar MM, Tahir M. 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