{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T11:11:46Z","timestamp":1790853106908,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2008,3,31]],"date-time":"2008-03-31T00:00:00Z","timestamp":1206921600000},"content-version":"vor","delay-in-days":30,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Contrast Media & Molecular"],"published-print":{"date-parts":[[2008,3]]},"abstract":"Abstract<\/jats:title>Objective<\/jats:title>The aim of this study was to evaluate in vivo<\/jats:italic> magnetic resonance imaging (MRI) for tracking the magnetically labeled mesenchymal stem cells (MSCs) transplanted into rat liver through hepatic arterial injection.<\/jats:p><\/jats:sec>Materials and Methods<\/jats:title>MSCs, harvested from the bone marrow of Wistar rats and expanded by the adhesion method, were labeled with both Feridex and 4\u2032,6\u2010diamidino\u20102\u2010phenylindole (DAPI). Cell transplantation was performed by injection of 1\u2009\u00d7\u2009106<\/jats:sup> labeled cells (n<\/jats:italic>\u2009=<\/jats:italic>\u200920) or unlabeled cells (n<\/jats:italic>\u2009=<\/jats:italic>\u200910) via hepatic artery into rat livers treated with 2% carbon tetrachloride to induce acute liver necrosis. MR imaging was performed on a clinical 1.5\u2009T MR scanner with a T<\/jats:italic>2<\/jats:sub>*\u2010weighted gradient\u2010echo sequence immediately before and at 1\u2009h, 3 days, 7 days and 14 days after transplantation, and the signal\u2010to\u2010noise ratios (SNRs) were measured in liver, spleen, kidney and muscle. After MR examination, the animals were sacrificed, and the liver, kidney, lung and muscle were prepared for fluorescence observation and Prussian Blue staining.<\/jats:p><\/jats:sec>Results<\/jats:title>In the group treated with labeled cells, the SNR of the liver after cell transplantation was 3.12\u2009\u00b1\u20090.43 at 1\u2009h, 7.98\u2009\u00b1\u20091.05 at 3 days and 11.46\u2009\u00b1\u20091.41 at 7 days. These values were significantly lower than the pre\u2010transplantation SNR (14.40\u2009\u00b1\u20090.37). In the group treated with unlabeled cells, no significant difference could be found between after and before transplantation liver SNRs. Prussian Blue staining showed iron particles, contained within the cytoplasm and distributed in the liver parenchyma, which corresponded to the DAPI\u2010stained fluorescent nuclei under the fluorescence microscope.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>The magnetically labeled MSCs transplanted into rat liver through hepatic arterial injection can be detected and monitored in vivo<\/jats:italic> with a 1.5\u2009T clinical MR scanner for up to 7 days after cell transplantation. 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