{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:27:44Z","timestamp":1791300464595,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2010,5,7]],"date-time":"2010-05-07T00:00:00Z","timestamp":1273190400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Contrast Media &amp; Molecular"],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Imaging of macrophages with superparamagnetic iron oxide particles (SPIO) has been performed to improve detection of atherosclerotic plaque inflammation in human and mouse studies by molecular magnetic resonance imaging (MRI). Since affinity of the monocyte\/macrophage integrin MAC\u20101 (CD11b\/CD18) is upregulated in inflammation, we generated a contrast agent targeting CD11b (CD11b\u2010SPIOs) for improved macrophage detection in plaques. CD11b\u2010SPIOs and non\u2010targeted SPIOs (control\u2010SPIOs) were incubated <jats:italic>in vitro<\/jats:italic> with human monocytes\/macrophages. As quantified by SPIO\u2010induced MRI signal extinction, intracellular iron\u2010content was significantly higher in monoytes\/macrophages incubated with CD11b\u2010SPIO than with control\u2010SPIO <jats:italic>in vitro<\/jats:italic> (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05), suggesting an improved uptake of CD11b\u2010SPIOs into monocytes. Therefore, the aortic arch (AA) and vessel branches of ApoE<jats:sup>\u2212\/\u2212<\/jats:sup>\u2010knockout mice on a Western\u2010type diet were imaged before and 48\u2009h after contrast agent injection of either CD11b\u2010SPIOs or control\u2010SPIOs, using a 9.4 T animal MRI system. The SPIO\u2010induced change in the MRI signal was quantified, as well as the macrophage\u2010content by anti\u2010CD68 immunhistochemistry and the iron\u2010content by Prussian\u2010blue staining. However, SPIO\u2010induced signal extinction in <jats:italic>in vivo<\/jats:italic>\u2010MRI was similar in CD11b\u2010SPIO and control\u2010SPIO\u2010injected animals, with a non\u2010significant trend towards an improved uptake of CD11b\u2010SPIOs in the subclavian artery and subsections of the AA. These data correlated well with the results obtained by histology. Although <jats:italic>in vitro<\/jats:italic> MRI\u2010data indicated an increased uptake of targeted CD11b\u2010SPIOs in monocytes\/macrophages, <jats:italic>in vivo<\/jats:italic> mouse data do not allow improved atherosclerotic plaque detection compared WITH non\u2010targeted SPIOs. Therefore, CD11b\u2010targeted MRI contrast labelling of monocytes\/macrophages does not seem to be a successful strategy in stable atherosclerotic plaques such as found in the ApoE<jats:sup>\u2212\/\u2212<\/jats:sup>\u2010knockout\u2010model. However, the impressive correlation between MRI and histology data encourages further development of inflammation\u2010 and plaque\u2010specific contrast agents for vulnerable plaque imaging. Copyright \u00a9 2010 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/cmmi.384","type":"journal-article","created":{"date-parts":[[2010,5,7]],"date-time":"2010-05-07T19:25:09Z","timestamp":1273260309000},"page":"268-275","source":"Crossref","is-referenced-by-count":21,"title":["Imaging monocytes with iron oxide nanoparticles targeted towards the monocyte integrin MAC\u20101 (CD11b\/CD18) does not result in improved atherosclerotic plaque detection by <i>in vivo<\/i> MRI"],"prefix":"10.1002","volume":"5","author":[{"given":"C.","family":"von zur Muhlen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Fink\u2010Petri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Salaklang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Paul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Neudorfer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Berti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Merkle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Peter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Bode","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"von Elverfeldt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,5,7]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9343(02)01344-X"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra043430"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.108.165498"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrd1548"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.0000131268.50418.b7"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000222920.59760.df"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1076-6332(03)80422-1"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000068315.98705.CC"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(02)00440-4"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000055323.57885.88"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.rli.0000101027.57021.28"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2006.08.048"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0606281104"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.acthis.2006.07.002"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000146529.68729.8b"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1080\/15419060302065"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000219687.71607.f7"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.915"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI28549"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.108.180521"},{"key":"e_1_2_7_22_2","unstructured":"BremickerJ KlugG KampfT BauerE Basse\u2010L\u00fcsebrinkT WeberM GbureckU N\u00f6thU JakobP BauerWR.Detection limits of very small iron oxide nanoparticles in labeled cells: a quantitative evaluation of histochemistry and MR\u2010relaxometry. 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