{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T08:27:01Z","timestamp":1765700821348,"version":"build-2065373602"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2001,12,28]],"date-time":"2001-12-28T00:00:00Z","timestamp":1009497600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer"],"published-print":{"date-parts":[[2002,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>It is known that proliferation is deregulated progressively during carcinogenesis in Barrett esophagus (BE). Cyclin B1 is a key protein for the regulation of G2\u2010M\u2010phase transition during the cell cycle and is essential for initiation of mitosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>Using immunohistochemistry, samples of Barrett metaplastic specialized epithelium (SE; <jats:italic>n<\/jats:italic> = 36 samples), low\u2010grade dysplasia (LGD; <jats:italic>n<\/jats:italic> = 25 samples), high\u2010grade dysplasia (HGD; <jats:italic>n<\/jats:italic> = 25 samples), and invasive adenocarcinoma (CA; <jats:italic>n<\/jats:italic> = 46 samples) derived from 50 esophagectomy specimens were investigated for the expression of cyclin B1. The number of cyclin B1 positive cells was determined semiquantitatively. In addition, in SE, LGD, and HGD samples, the pattern of cyclin B1 expression was assessed by determination of the presence of positive cells in four mucosal compartments: the deep glandular zone, the lower crypt zone, the upper crypt zone, and the luminal surface.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Cyclin B1 expression was found in all lesions under investigation. Regarding the percentage of positive cells, a marked increase of cyclin B1 positive cells was observed in SE samples compared with LGD samples and in HGD samples compared with CA samples (chi\u2010square test; <jats:italic>P<\/jats:italic> &lt; 0.0001), nevertheless showing a broad overlap between the different lesions. Concerning staining patterns, in the majority of SE samples (72.2%), cyclin B1 positive cells were restricted to the glandular zone and the lower crypt zone. In contrast, an expansion of cyclin B1 positive cells to superficially located zones of the mucosa (the upper crypt zone and\/or the luminal surface) was observed in the majority of LGD samples (96.0%) and HGD samples (100%; <jats:italic>P<\/jats:italic> &lt; 0.0001).<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>Overexpression of cyclin B1 is a frequent and early finding in the metaplasia\u2010dysplasia\u2010carcinoma sequence in BE. It may contribute to the loss of growth control and, subsequently, to the development of tumors in this location. Cancer 2002;92:212\u20138. \u00a9 2002 American Cancer Society.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncr.10152","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T18:14:37Z","timestamp":1030299277000},"page":"212-218","source":"Crossref","is-referenced-by-count":26,"title":["Expression of cyclin B1 in the metaplasia\u2010dysplasia\u2010carcinoma sequence of Barrett esophagus"],"prefix":"10.1002","volume":"94","author":[{"given":"Helene","family":"Geddert","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans J.","family":"Heep","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helmut E.","family":"Gabbert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario","family":"Sarbia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,12,28]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0046-8177(94)90057-4"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65346-1"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/00042737-199912000-00003"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1295-1245"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-230X(08)60352-8"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.115.1.1"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevEukarGeneExpr.v5.i2.20"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.61.070192.002301"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1998.4352"},{"key":"e_1_2_6_11_2","first-page":"4285","article-title":"Unscheduled expression of cyclin B1 and cyclin E in several leukemic and solid tumor cell lines","volume":"54","author":"Gong J","year":"1994","journal-title":"Cancer Res"},{"key":"e_1_2_6_12_2","first-page":"811","article-title":"Cyclin\u2010dependent kinase activation and S\u2010phase induction of the cyclin B1 gene are linked through the CCAAT elements","volume":"8","author":"Katula KS","year":"1997","journal-title":"Cell Growth Diff"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(19990401)85:7<1569::AID-CNCR19>3.0.CO;2-M"},{"key":"e_1_2_6_14_2","first-page":"15","article-title":"Expression of the G2\u2010M checkpoint regulators cyclin B1 and cdc2 in nonmalignant and malignant human breast lesions: immunocytochemical and quantitative image analyses","volume":"150","author":"Kawamoto H","year":"1997","journal-title":"Am J Pathol"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1999.tb02000.x"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01269891"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004280050319"},{"volume-title":"Classification of tumours. 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