{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T00:27:19Z","timestamp":1760315239841,"version":"build-2065373602"},"reference-count":16,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2001,12,28]],"date-time":"2001-12-28T00:00:00Z","timestamp":1009497600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer"],"published-print":{"date-parts":[[2002,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Telomerase is a nucleoprotein complex that caps the physical termini of all eukaryotic chromosomes. Because most malignant cells and reproductive cells have telomerase activity, which elongates telomeric DNA, telomerase may play important roles in unlimited cell division acquisition of the malignant phenotype. The current study examined the relation of telomerase activity in thymoma and thymic carcinoma with the clinicopathologic features of these lesions.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>Tissue specimens were surgically resected from patients with thymoma and thymic carcinoma. Telomerase activity was evaluated according to a modified telomeric repeat amplification protocol assay. Paraffin sections of tumor were immunostained by MIC2 antibody, a marker of immature T cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Telomerase activity was detected in all thymic epithelial tumors. The activity (mean \u00b1 SD; unit per \u03bcg protein) in thymoma (<jats:italic>n<\/jats:italic> = 17) was significantly higher than that in thymic carcinoma (<jats:italic>n<\/jats:italic> = 7) (431.8 \u00b1 400.1 vs. 68.8 \u00b1 39.8; <jats:italic>P<\/jats:italic> &lt; 0.01). Telomerase activities in thymoma and thymic carcinoma were significantly higher than that in primary lung adenocarcinoma (33.5 \u00b1 39.2, <jats:italic>n<\/jats:italic> = 47), studied as a control (<jats:italic>P<\/jats:italic> &lt; 0.01). In patients with thymoma, telomerase activity did not correlate with tumor stage according to Masaoka classification (<jats:italic>P<\/jats:italic> = 0.776). In patients with thymic carcinoma, however, telomerase activity positively correlated with tumor stage (<jats:italic>P<\/jats:italic> = 0.02). In thymoma, telomerase activity positively correlated with the ratio of induced lymphocytes according to Rosai's classification (<jats:italic>P<\/jats:italic> = 0.045). MIC2\u2010positive lymphocytes were identified in all cases of thymoma (<jats:italic>n<\/jats:italic> = 12). In contrast, lymphocytes infiltrating thymic carcinoma did not react with MIC2.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>In thymoma, telomerase activity reflects the presence of immature T\u2010cell lymphocytes in tumor tissue rather than tumor stage or malignant phenotype. In thymic carcinoma, telomerase activity derived directly from cancer cells may relate to tumor stage. Cancer 2002;94:240\u20134. \u00a9 2002 American Cancer Society.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncr.10194","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T18:14:37Z","timestamp":1030299277000},"page":"240-244","source":"Crossref","is-referenced-by-count":9,"title":["Enhanced expression of telomerase activity in thymoma and thymic carcinoma tissues"],"prefix":"10.1002","volume":"94","author":[{"given":"Masazumi","family":"Watanabe","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shan\u2010kang","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makoto","family":"Sawafuji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masafumi","family":"Kawamura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirohisa","family":"Horinouchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makio","family":"Mukai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koichi","family":"Kobayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,12,28]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7605428"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/89.21.1609"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-199510000-00002"},{"key":"e_1_2_5_5_2","series-title":"Atlas of Tumor Pathology","volume-title":"Tumor of the thymus","author":"Rosai J","year":"1975"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19811201)48:11<2485::AID-CNCR2820481123>3.0.CO;2-R"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(20000401)88:7<1524::AID-CNCR4>3.0.CO;2-6"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(20000115)88:2<312::AID-CNCR10>3.0.CO;2-R"},{"key":"e_1_2_5_9_2","first-page":"1931","article-title":"Telomerase and telomere length in the development and progression of premalignant lesions to colorectal cancer","volume":"3","author":"Engelhardt M","year":"1997","journal-title":"Clin Cancer Res"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/109.2.153"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0344-0338(99)80117-4"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.20.9082"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.183.6.2471"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V88.1.222.222"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.9.5147"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1201933"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/554"}],"container-title":["Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcncr.10194","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/acsjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cncr.10194","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T10:25:01Z","timestamp":1760264701000},"score":1,"resource":{"primary":{"URL":"https:\/\/acsjournals.onlinelibrary.wiley.com\/doi\/10.1002\/cncr.10194"}},"subtitle":["A clinicopathologic study"],"short-title":[],"issued":{"date-parts":[[2001,12,28]]},"references-count":16,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2002,1]]}},"alternative-id":["10.1002\/cncr.10194"],"URL":"https:\/\/doi.org\/10.1002\/cncr.10194","archive":["Portico"],"relation":{},"ISSN":["0008-543X","1097-0142"],"issn-type":[{"type":"print","value":"0008-543X"},{"type":"electronic","value":"1097-0142"}],"subject":[],"published":{"date-parts":[[2001,12,28]]}}}