{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:12:13Z","timestamp":1762099933693,"version":"build-2065373602"},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2002,7,22]],"date-time":"2002-07-22T00:00:00Z","timestamp":1027296000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer"],"published-print":{"date-parts":[[2002,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>p53 mutations are an early event in the multistep progression of gastric carcinoma. These mutations are often present in dysplastic and intestinal metaplastic gastric epithelium. However, the presence of immunohistochemically detectable p53 protein and p53 mutations in nondysplastic\/nonmetaplastic gastric mucosa is more controversial. Recent reports have suggested that immunohistochemically detectable p53 protein may be present in the gastric epithelium of <jats:italic>Helicobacter pylori<\/jats:italic> gastritis. Furthermore, because cagA\u2010positive <jats:italic>H. pylori<\/jats:italic> is associated with greater mucosal injury but decreased apoptosis, it would be interesting to determine if this phenotype is associated with greater immunostaining of p53, as the wild\u2010type p53 gene helps to initiate apoptosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>One hundred thirty\u2010five patients with <jats:italic>H. pylori<\/jats:italic>\u2010associated gastritis were immunohistochemically stained for p53 and quantified for the extent and intensity of the staining using a semiquantitative method (0, nil staining; 6, extensive and strong staining). The cagA status of the organism was determined by Western blot.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Thirty\u2010one patients (23%) showed strong p53 staining (\u2265 4 of 6) in inflamed but otherwise normal gastric epithelium. In the 123 cagA\u2010positive <jats:italic>H. pylori<\/jats:italic> gastritis patients, the average p53 staining score was 2.5 of 6. This is significantly higher than the corresponding score of 1.7 of 6 observed in the 12 patients with cagA\u2010negative <jats:italic>H. pylori<\/jats:italic> gastritis (<jats:italic>P<\/jats:italic> = 0.04).<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>Our results indicate that p53 protein is immunohistochemically detectable even before gastric metaplastic\/dysplastic change occurs. The results also suggest that cagA\u2010positive <jats:italic>H. pylori<\/jats:italic> might be associated with greater p53 immunohistochemical staining. This would indicate that p53 immunohistochemical staining does not reliably differentiate between gastric dysplasia and reactive inflammatory atypia. If the p53 protein detected is a consequence of mutation, this would help to explain why cagA\u2010positive <jats:italic>H. pylori<\/jats:italic> gastritis is associated with decreased apoptosis. Cancer 2002;95:499\u2013505. \u00a9 2002 American Cancer Society.<\/jats:p><jats:p>DOI 10.1002\/cncr.10697<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncr.10697","type":"journal-article","created":{"date-parts":[[2002,10,6]],"date-time":"2002-10-06T07:53:08Z","timestamp":1033890788000},"page":"499-505","source":"Crossref","is-referenced-by-count":25,"title":["Study of p53 immunostaining in the gastric epithelium of cagA\u2010positive and cagA\u2010negative <i>Helicobacter pylori<\/i> gastritis"],"prefix":"10.1002","volume":"95","author":[{"given":"Ming","family":"Teh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kong","family":"Bing Tan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bee","family":"Leng Seet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khay","family":"Guan Yeoh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,7,23]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2036.1998.00002.x"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/00313029400169142"},{"key":"e_1_2_6_4_2","first-page":"223","article-title":"p53 gene mutations and protein nuclear accumulation are early events in intestinal type gastric cancer but late events in diffuse type","volume":"4","author":"Ranzani GN","year":"1995","journal-title":"Cancer Epidemiol Biomarkers Prev."},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.35.12.1697"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910620207"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19960220)69:1<28::AID-IJC6>3.0.CO;2-Y"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19960529)66:5<594::AID-IJC2>3.0.CO;2-O"},{"key":"e_1_2_6_9_2","first-page":"511","article-title":"P53 alteration in gastric precancerous lesions","volume":"144","author":"Shiao Y\u2010H","year":"1994","journal-title":"Am J Pathol."},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/108.1.26"},{"key":"e_1_2_6_11_2","first-page":"1695","article-title":"Increase in proliferation and apoptosis of gastric epithelial cells early in the natural history of Helicobacter pylori infection","volume":"151","author":"Jones NL","year":"1997","journal-title":"Am J Pathol."},{"key":"e_1_2_6_12_2","unstructured":"ChiaKS SeowA LeeHP ShanmugaratnamK.Cancer incidence in Singapore 1993\u20131997. 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