{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T16:47:15Z","timestamp":1782578835321,"version":"3.54.5"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2002,12,18]],"date-time":"2002-12-18T00:00:00Z","timestamp":1040169600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer"],"published-print":{"date-parts":[[2003,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>The human matrix metalloproteinase (MMP)\u201026, also called matrilysin\u20102 or endometase, has been isolated as a matrilysin (MMP\u20107) homolog. Matrix metalloproteinase\u201026 was expressed in tissue samples from the placenta and endometrial tumors and its expression may be related to the development of endometrial carcinomas.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>Total RNAs were isolated from 5 endometrial carcinoma cell lines, 36 normal endometrial tissue samples, 4 hyperplasia tissue samples, and from 24 endometrial carcinoma tissue samples. Reverse transcription\u2010polymerase chain reation (RT\u2010PCR) was performed to detect MMP\u201026 mRNA expression. To identify MMP\u201026 mRNA localization and protein expression, we performed in situ RT\u2010PCR and immunohistochemistry, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Reverse transcription\u2010polymerase chain reaction analysis revealed that MMP\u201026 mRNA was expressed in 24 of 36 normal human endometrial tissue samples. However, MMP\u201026 mRNA expression was not detected in endometrial carcinoma cell lines nor in endometrial carcinoma tissue samples except for one case. Western blot analysis showed similar results. In situ RT\u2010PCR analysis revealed that MMP\u201026 expression was localized in the epithelial glandular cells but faint expression was observed in the stromal cells. Subsequently, we separated endometrial tissues into epithelial glandular and stromal cells. Using RT\u2010PCR, the purified epithelial glandular cells exhibited MMP\u201026 mRNA expression but the purified stromal cells did not. Immunohistochemical analyses revealed that MMP\u201026 protein expression is also limited to endometrial epithelial glandular cells but not to cancer cells. Therefore, MMP\u201026 expression is limited to normal epithelial glandular cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>We found a significant difference in MMP\u201026 expression in normal and malignant endometrial tissue samples, although its function is still unknown. These data suggest that MMP\u201026 may be a candidate for a new tumor marker for endometrial carcinomas. Cancer 2003;97:79\u201389. \u00a9 2003 American Cancer Society.<\/jats:p><jats:p>DOI 10.1002\/cncr.11030<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncr.11030","type":"journal-article","created":{"date-parts":[[2002,12,19]],"date-time":"2002-12-19T08:57:58Z","timestamp":1040288278000},"page":"79-89","source":"Crossref","is-referenced-by-count":39,"title":["Matrix metalloproteinase\u201026 is expressed in human endometrium but not in endometrial carcinoma"],"prefix":"10.1002","volume":"97","author":[{"given":"Keiichi","family":"Isaka","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirotaka","family":"Nishi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiromi","family":"Nakai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshihide","family":"Nakada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yin","family":"Feng Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiro","family":"Ebihara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaomi","family":"Takayama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,12,18]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.31.21491"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M002349200"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1699-0463.1999.tb01536.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cb.09.110193.002545"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0496-461"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2000.18.5.1135"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/89.17.1260"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81009-0"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.8.4570"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.2000.01363.x"},{"issue":"3","key":"e_1_2_6_12_2","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1042\/bj3560705","article-title":"Characterization of matrix metalloproteinase\u201026, a novel metalloproteinase widely expressed in cancer cells of epithelial origin","volume":"356","author":"Marchenko GN","year":"2001","journal-title":"Biochem J."},{"key":"e_1_2_6_13_2","first-page":"4745","article-title":"Matrilysin\u20102, a new matrix metalloproteinase expressed in human tumors and showing the minimal domain organization required for secretion, latency, and activity","volume":"60","author":"Uria JA","year":"2000","journal-title":"Cancer Res."},{"key":"e_1_2_6_14_2","first-page":"43","article-title":"Establishment and characterization of the new cell line (EI) from a human endometrial adenocarcinoma","volume":"8","author":"Yudate T","year":"1995","journal-title":"Hum Cell."},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(89)90787-4"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0090-8258(84)90080-5"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0039-128X(80)90108-7"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-48-4-639"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19991022)84:5<470::AID-IJC4>3.0.CO;2-D"},{"key":"e_1_2_6_20_2","first-page":"586","article-title":"Enhancement of mRNA in situ hybridization signal by microwave heating","volume":"73","author":"Sibony M","year":"1995","journal-title":"Lab Invest."},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2744(199806)22:2<84::AID-MC3>3.0.CO;2-K"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/15.suppl_3.57"},{"issue":"2","key":"e_1_2_6_23_2","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1042\/bj3630253","article-title":"Promoter characterization of the novel human matrix metalloproteinase\u201026 gene: regulation by the T\u2010cell factor\u20104 implies specific expression of the gene in cancer cells of epithelial origin","volume":"363","author":"Marchenko GN","year":"2002","journal-title":"Biochem J."},{"key":"e_1_2_6_24_2","first-page":"319","article-title":"Experience with Endopap endometrial sampling in the cytodiagnosis of endometrial carcinoma and its precursor lesions. 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