{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T07:08:08Z","timestamp":1770188888890,"version":"3.49.0"},"reference-count":25,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2003,4,1]],"date-time":"2003-04-01T00:00:00Z","timestamp":1049155200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer"],"published-print":{"date-parts":[[2003,4,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Nasopharyngeal carcinoma (NPC) is a common head and neck cancer in Taiwan. The goals of the current study were to investigate whether a nasopharyngeal swab technique could provide enough DNA for polymerase chain reaction (PCR) analysis of the Epstein\u2013Barr virus (EBV)\u2013derived latent membrane protein 1 (<jats:italic>LMP\u20101<\/jats:italic>) gene and to determine the feasibility and reliability of diagnosing NPC by detection of <jats:italic>LMP\u20101<\/jats:italic> in the nasopharynx.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>320 adults underwent nasopharyngoscopy and nasopharyngeal swab to obtain cells for the <jats:italic>LMP\u20101<\/jats:italic> PCR assay; some patients also underwent nasopharyngeal biopsy.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>An amount of DNA that was sufficient for PCR was extracted from 96.3% of the swab samples. By detecting <jats:italic>LMP\u20101<\/jats:italic> in nasopharyngeal swabs, NPC was diagnosed with a false positive rate of 12.7% (7 of55 patients), a false negative rate of 1.6% (4 of 253 patients), sensitivity of 87.3% (48 of 55 patients), specificity of 98.4% (249 of 253 patients), a positive predictive value of 92.3% (48 of 52 patients), and a negative predictive value of 97.3% (249\/256 patients). NPC was diagnosed by nasopharyngoscopy with a false positive rate of 38% (30 of 79 patients), a false negative rate of 0.4% (1 of 241 patients), sensitivity of 62% (49 of 79 patients), specificity of 99.6% (240 of 241 patients), a positive predictive value of 98% (49 of 50 patients), and a negative predictive value of 88.9% (240 of 270 patients). Only 7 (0.2%) of 256 patients with a diagnosis other than NPC had <jats:italic>LMP\u20101<\/jats:italic> detected in the nasopharyngeal space.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>Detecting EBV genomic <jats:italic>LMP\u20101<\/jats:italic> by nasopharyngeal swab diagnosed NPC with 87.3% sensitivity and 98.4% specificity. EBV genomic DNA usually is not detected by PCR\u2010based methods in the nasopharyngeal space. Its incidence is estimated to be as low as 0.2% among the general population. The nasopharyngeal swab coupled with PCR\u2010based EBV <jats:italic>LMP\u20101<\/jats:italic> detection could serve as part of a screening program for high\u2010risk populations. Cancer 2003;97:1909\u201313. \u00a9 2003 American Cancer Society.<\/jats:p><jats:p>DOI 10.1002\/cncr.11312<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncr.11312","type":"journal-article","created":{"date-parts":[[2003,4,2]],"date-time":"2003-04-02T12:37:22Z","timestamp":1049287042000},"page":"1909-1913","source":"Crossref","is-referenced-by-count":40,"title":["Screening nasopharyngeal carcinoma by detection of the latent membrane protein 1 <i>(LMP\u20101)<\/i> gene with nasopharyngeal swabs"],"prefix":"10.1002","volume":"97","author":[{"given":"Sheng\u2010Po","family":"Hao","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ngan\u2010Ming","family":"Tsang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kai\u2010Ping","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,4]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0360-3016(85)90033-1"},{"key":"e_1_2_5_3_2","first-page":"72","volume-title":"Leading causes of death from cancer in the Taiwan area from 1991 to 1996. 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