{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T23:17:21Z","timestamp":1774135041029,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,11,22]],"date-time":"2004-11-22T00:00:00Z","timestamp":1101081600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer"],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Imatinib mesylate, a BCR\u2010ABL tyrosine kinase inhibitor, induces apoptosis in chronic myeloid leukemia cells. Resistance to imatinib is currently the most important concern of this treatment. One of the main mechanisms of this resistance is overexpression of BCR\u2010ABL.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>In the current study, the authors investigated the correlation between BCR\u2010ABL overexpression and apoptosis in BaF\/<jats:italic>BCR<\/jats:italic>\u2010<jats:italic>ABL<\/jats:italic> and LAMA84 cell lines resistant to imatinib suddenly deprived of the inhibitor, and compared with their sensitive counterpart.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Removal of imatinib from culture medium led to a decrease in Bcr\u2010Abl protein expression by Day 5, which was sustained for \u2265 3 weeks of imatinib deprivation. Apoptosis was observed after 3 days of imatinib deprivation in resistant lines accompanied by caspase activation, loss of membrane asymmetry (annexin V staining), and alteration of mitochondrial potential (dihexyloxacarbocyanine iodide [DiOC6]). Transient activation of the STAT5\/Bcl\u2010xL pathway and Akt kinase activity preceded these responses.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>Thus, imatinib removal led to apoptosis of BCR\u2010ABL\u2013overexpressing leukemic cells, a phenomenon that could be exploited to sensitize imatinib\u2010resistant cells to the cytotoxic effect of other drugs. Cancer 2005. \u00a9 2004 American Cancer Society.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncr.20758","type":"journal-article","created":{"date-parts":[[2004,11,22]],"date-time":"2004-11-22T14:25:40Z","timestamp":1101133540000},"page":"102-110","source":"Crossref","is-referenced-by-count":10,"title":["Overproduction of BCR\u2010ABL induces apoptosis in imatinib mesylate\u2010resistant cell lines"],"prefix":"10.1002","volume":"103","author":[{"given":"Vanessa","family":"Desplat","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francis","family":"Belloc","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Val\u00e9rie","family":"Lagarde","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Boyer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junia 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