{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T02:23:08Z","timestamp":1785896588727,"version":"3.56.0"},"reference-count":60,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2005,4,13]],"date-time":"2005-04-13T00:00:00Z","timestamp":1113350400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer"],"published-print":{"date-parts":[[2005,5,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>It was reported previously that malignant human tumors, like glioma and medulloblastoma, express high\u2010density interleukin (IL\u20104) receptor mRNA and protein. Because IL\u20104 receptors (R) are sensitive targets for targeted therapeutics, knowledge of the expression of these receptors in other central nervous system tumors is of great interest. In this study, the authors examined the expression and subunit composition of IL\u20104R complex in primary human meningiomas.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>Reverse transcription\u2010polymerase chain reaction (RT\u2010PCR) analysis for IL\u201013R\u03b11, IL\u20104R\u03b1 and IL\u20102R\u03b3c was performed on total RNA extracted from 35 meningiomas and a normal human brain tissue sample. Results were confirmed in nine randomly selected tumors by quantitative real\u2010time PCR and in situ immunofluorescence assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Transcripts for the IL\u20104R\u03b1 and IL\u201013R\u03b11 chains were overexpressed in meningiomas compared with normal brain tissue. The levels of IL\u20104R\u03b1 mRNA appeared to be higher compared with the levels of IL\u201013R\u03b11 mRNA. The results also showed that tumors with higher disease grade tended to have increased mRNA expression for the IL\u20104R\u03b1 chain. This IL\u20104R\u03b1 mRNA overexpression appeared to be more frequent in younger patients (age &lt; 37 years). The transcripts for IL\u20102R\u03b3c chain were not detected in any of the tumor samples or in normal brain tissue. Quantitative real\u2010time PCR confirmed the results of the RT\u2010PCR analysis. Meningiomas also demonstrated a bright immunofluorescent staining for the IL\u20104R\u03b1 and IL\u201013R\u03b11 chains but no staining for IL\u20102R\u03b3c.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>Expression of the IL\u20104R\u03b1 and IL\u201013R\u03b11 chains and absence of IL\u20102\u03b3c expression established that meningiomas expressed type II IL\u20104Rs. These receptors may serve as a target for cytotoxin\/immunotoxin therapy in patients with meningioma who are not amenable to surgical resection or for recurrent tumors. Cancer 2005. \u00a9 2005 American Cancer Society.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncr.21008","type":"journal-article","created":{"date-parts":[[2005,4,13]],"date-time":"2005-04-13T19:17:13Z","timestamp":1113419833000},"page":"2132-2142","source":"Crossref","is-referenced-by-count":17,"title":["Expression and structure of interleukin 4 receptors in primary meningeal tumors"],"prefix":"10.1002","volume":"103","author":[{"given":"Sachin","family":"Puri","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bharat H.","family":"Joshi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chitra","family":"Sarkar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashok Kumar","family":"Mahapatra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ejaz","family":"Hussain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Subrata","family":"Sinha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,4,13]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"1165","article-title":"Epidermal growth factor expression in brain neoplasms","volume":"34","author":"Och W","year":"2000","journal-title":"Neurol Neurochir Pol."},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.3171\/jns.1997.87.2.0315"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.3171\/jns.2001.94.3.0487"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.8.3735"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/20.19.5173"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.155.3.914"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.163.6.1583"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2935936"},{"key":"e_1_2_6_10_2","doi-asserted-by":"crossref","first-page":"1310","DOI":"10.4049\/jimmunol.143.4.1310","article-title":"Regulation of human basophil mediator release by cytokines. 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