{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T02:47:26Z","timestamp":1791254846723,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,8,2]],"date-time":"2005-08-02T00:00:00Z","timestamp":1122940800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer"],"published-print":{"date-parts":[[2005,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>To establish the prognostic value of immune system cells that infiltrate melanoma, the authors evaluated the distribution and density of T lymphocyte subsets, macrophages, and dendritic cells in samples of primary cutaneous melanoma from 47 patients with Stage I and II melanoma according to the American Joint Committee on Cancer staging system.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>Immunohistochemical demonstrations of CD8 and CD4 lymphocytes, CD68 macrophages, human leukocyte antigen\u2010D\u2010related (HLA\u2010DR) cells, S\u2010100 protein, and melanoma\u2010associated antigens Melan A and HMB\u201045 were performed. The results were derived from independent histopathologic reviews by two pathologists. The low\u2010density, moderate\u2010density, and high\u2010density groups of cells that infiltrated the base of the tumor during the vertical growth phase were compared with the overall survival rate using the Kaplan\u2013Meier method and the log\u2010rank test. Clinical variables (gender, age, tumor location, Clark level, vascular\/lymphatic invasion, and thickness) also were analyzed.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>The CD8 lymphocytes exhibited independent statistically positive significance in survival (log\u2010rank test, 8.49; <jats:italic>P<\/jats:italic> = 0.01) between patients in different lymphocyte density groups. There was a difference in 5\u2010year survival among patients in the high\u2010density group (78.8%), the moderate\u2010density group (44.4%), and the low\u2010density group (25.0%). The CD4 lymphocytes, which were less numerous than CD8 cells, had similar distribution. There also was a correlation of HLA\u2010DR cells with overall survival (log\u2010rank test, 5.29; <jats:italic>P<\/jats:italic> = 0.02). CD68 cell density was not found to be correlated with survival.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>The presence and number of infiltrating CD8 lymphocytes as well as the overall occurrence of HLA\u2010DR cells may be considered independent, favorable prognostic factors in melanoma. The current results may be important for identifying other prognostic factors with which to evaluate disease progression and develop immune therapies for patients with melanoma. 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