{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T23:40:20Z","timestamp":1790206820083,"version":"4.1.0"},"reference-count":15,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,12,9]],"date-time":"2005-12-09T00:00:00Z","timestamp":1134086400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer"],"published-print":{"date-parts":[[2006,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Whether stool DNA abnormalities arise solely from colorectal neoplastic lesions or are due to more pervasive field effects is not known. In the current study, the authors conducted a prospective multicenter study to evaluate the performance of stool\u2010based DNA testing in a large cohort and to examine whether the findings before treatment persist after surgical resection and\/or adjuvant therapy.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>Patients with newly diagnosed colorectal carcinoma or advanced adenomas (AA) provided stool samples before therapy, 1\u20133 months after surgical resection, and 6\u20139 months postresection. Stool samples were analyzed using the multitarget DNA assay panel (MTAP) consisting of 23 markers: 21 mutations in the <jats:italic>p53<\/jats:italic>, K\u2010<jats:italic>ras<\/jats:italic>, and <jats:italic>APC<\/jats:italic> genes, a microsatellite instability marker (BAT\u201026), and the DNA integrity assay (DIA), a marker of loss of apoptosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Overall, 49 of 91 individuals (54%) tested positive with the MTAP test. The sensitivity of the MTAP test was 63% for invasive tumors compared with 26% for AA. Individuals whose lesions had a more advanced TNM stage or were located distal to the splenic flexure were significantly more likely to have a positive MTAP test. Of the 79 samples collected at 1\u20133 months after surgical resection of the neoplasm, 14 (18%) had a positive MTAP result, 12 of which were positive for DIA only. Of those collected at 6\u20139 months of follow\u2010up, 5 of 72 (7%) tested positive on the MTAP panel.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>Although many samples collected 1\u20133 months after surgical resection of the colorectal neoplasm tested positive on the MTAP, most were negative by 6\u20139 months, indicating that stool DNA abnormalities disappear after treatment of the neoplastic lesions. Surgery and chemoradiation appear to induce transient DIA abnormalities that may be independent of the presence of neoplasia. Cancer 2006. \u00a9 2005 American Cancer Society.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncr.21558","type":"journal-article","created":{"date-parts":[[2005,12,9]],"date-time":"2005-12-09T11:46:49Z","timestamp":1134128809000},"page":"277-283","source":"Crossref","is-referenced-by-count":35,"title":["Detection of stool DNA mutations before and after treatment of colorectal neoplasia"],"prefix":"10.1002","volume":"106","author":[{"given":"Sapna","family":"Syngal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Stoffel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Chung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Willett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Schoetz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Schroy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deepa","family":"Jagadeesh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathleen","family":"Morel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Ross","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,12,9]]},"reference":[{"key":"e_1_2_6_2_2","volume-title":"Cancer reference information","author":"American Cancer Society","year":"2005"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2000.16507"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/327298a0"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/327293a0"},{"key":"e_1_2_6_6_2","first-page":"654","article-title":"BAT\u201026 microsatellite instability is a sensitive indicator of mismatch repair mutation in hereditary non\u2010polyposis colorectal cancer","volume":"87","author":"Coombs NJ","year":"2000","journal-title":"Br J Surg."},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2000.19580"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/93.11.858"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa012294"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(20000101)85:1<73::AID-IJC13>3.0.CO;2-#"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.3816\/CCC.2003.n.011"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1053\/S1542-3565(03)00186-1"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa033403"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199911113412003"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa031618"},{"key":"e_1_2_6_16_2","first-page":"900","article-title":"Detection of aberrantly methylated hMLH1 promoter DNA in the serum of patients with microsatellite unstable colon cancer","volume":"61","author":"Grady WM","year":"2001","journal-title":"Cancer Res."}],"container-title":["Cancer"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcncr.21558","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/acsjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cncr.21558","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T19:42:10Z","timestamp":1760298130000},"score":1,"resource":{"primary":{"URL":"https:\/\/acsjournals.onlinelibrary.wiley.com\/doi\/10.1002\/cncr.21558"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,12,9]]},"references-count":15,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2006,1,15]]}},"alternative-id":["10.1002\/cncr.21558"],"URL":"https:\/\/doi.org\/10.1002\/cncr.21558","archive":["Portico"],"relation":{},"ISSN":["0008-543X","1097-0142"],"issn-type":[{"value":"0008-543X","type":"print"},{"value":"1097-0142","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,12,9]]}}}