{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T17:00:08Z","timestamp":1761843608877,"version":"build-2065373602"},"reference-count":41,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T00:00:00Z","timestamp":1171929600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer"],"published-print":{"date-parts":[[2007,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Nuclear p53 and retinoblastoma protein (pRb) were reported to be poor prognostic indicators for transitional cell carcinoma of the bladder. The authors sought to determine the prognostic value of nuclear p53 and pRb in superficial bladder transitional cell carcinoma patients who were treated with intravesical bacille Calmette\u2010Guerin (BCG) or BCG with interferon\u2010alpha (IFN\u2010\u03b1).<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>A prospective histological review was performed for 80 superficial bladder transitional cell carcinoma patients who underwent postresection intravesical regimes of BCG (81mg, n = 33 or 27 mg, n = 20) or BCG (27 mg) with IFN\u2010\u03b1 (n = 27), and followed for a mean of 4.5 years. Hematoxylin and eosin (H &amp; E) and immunoperoxidase staining were performed on tissue sections. Nuclear p53 and pRb immunoreactivity were assessed semiquantitatively, by using a combination of staining extent and intensity, to categorize overexpression or underexpression. Data were analyzed by using chi\u2010square analysis, multiple logistic regression, and Kaplan\u2010Meier curves.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>pRb expression was not associated with patient outcome after BCG\u2010alone therapy, but pRb underexpression was significantly associated with BCG nonresponse and tumor recurrence (<jats:italic>P<\/jats:italic> = .047) after BCG and IFN\u2010\u03b1 (BCG + IFN\u2010\u03b1) therapy. Low\u2010grade tumors were associated with pRb overexpression, with or without nuclear p53 underexpression (<jats:italic>P<\/jats:italic> = .019; <jats:italic>P<\/jats:italic> = .043, respectively). p53 expression alone or in combination with pRb expression had no significant relation with tumor response to BCG alone or BCG + IFN\u2010\u03b1 with respect to recurrence, progression, or cancer\u2010specific death.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>Nuclear pRb underexpression may be predictive of nonresponse and cancer recurrence after intravesical BCG + IFN\u2010\u03b1 therapy. Nuclear p53 expression or its combination with pRb expression is not associated with post\u2010BCG clinical outcome, so p53 expression or p53 with pRb expression should not be used to influence decisions concerning BCG\u2010alone or BCG + IFN\u2010\u03b1 therapy. Cancer 2007. \u00a9 2007 American Cancer Society.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncr.22503","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T15:49:50Z","timestamp":1171986590000},"page":"1097-1105","source":"Crossref","is-referenced-by-count":43,"title":["Predictive value of p53 and pRb expression in superficial bladder cancer patients treated with BCG and interferon\u2010alpha"],"prefix":"10.1002","volume":"109","author":[{"given":"Kesavan","family":"Esuvaranathan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edmund","family":"Chiong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas Paulraj","family":"Thamboo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiong 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