{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T20:17:31Z","timestamp":1791145051058,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2007,4,17]],"date-time":"2007-04-17T00:00:00Z","timestamp":1176768000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer"],"published-print":{"date-parts":[[2007,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND.<\/jats:title><jats:p><jats:italic>JAK2<\/jats:italic>V617F occurs in approximately 50% of patients with essential thrombocythemia (ET). Qualitative studies of mutation analysis have previously reported an association between <jats:italic>JAK2<\/jats:italic>V617F and advanced age, higher hemoglobin level, higher leukocyte count, and lower platelet count. A possible association with thrombotic complication has also been considered.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS.<\/jats:title><jats:p>Allele\u2010specific, quantitative polymerase chain reaction (PCR) analysis for <jats:italic>JAK2<\/jats:italic>V617F was performed in 176 patients with ET using genomic DNA from archived bone marrow, which was collected within 1 year (n = 72 patients), between 1 and 5 years (n = 64 patients), or after 5 years (n = 40 patients) of diagnosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS.<\/jats:title><jats:p><jats:italic>JAK2<\/jats:italic>V617F was detected in 96 patients (55%), in whom mutant allele burden ranged from 1% to 100% (median, 6.3%). Neither mutational frequency (<jats:italic>P<\/jats:italic> = .37) nor mutant allele burden (<jats:italic>P<\/jats:italic> = .62) was affected by the timing of bone marrow sample collection. The presence of <jats:italic>JAK2<\/jats:italic>V617F was found to be significantly associated with higher hemoglobin level (<jats:italic>P<\/jats:italic> &lt; .0001), lower platelet count (<jats:italic>P<\/jats:italic> = .001), higher leukocyte count (<jats:italic>P<\/jats:italic> = .008), increased incidence of venous thrombosis occurring after diagnosis (<jats:italic>P<\/jats:italic> = .02), and older age at diagnosis (<jats:italic>P<\/jats:italic> = .03). All but age retained significance in multivariable analysis. In mutation\u2010positive patients (n = 96 patients), <jats:italic>JAK2<\/jats:italic>V617F allele burden clustered between 1% and 22% in 94 cases, in whom it correlated directly and significantly with platelet and leukocyte counts, palpable splenomegaly at diagnosis, and venous thrombosis occurring after diagnosis. The latter 2 associations remained significant with the inclusion of the remaining 2 outlier cases with 100% mutant allele burden; in addition, an association with male gender became evident.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS.<\/jats:title><jats:p><jats:italic>JAK2<\/jats:italic>V617F allele burden imparts additional phenotypic effects in ET. Cancer 2007. \u00a9 2007 American Cancer Society.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncr.22663","type":"journal-article","created":{"date-parts":[[2007,4,17]],"date-time":"2007-04-17T16:48:07Z","timestamp":1176828487000},"page":"2279-2284","source":"Crossref","is-referenced-by-count":130,"title":["Clinical correlates of <i>JAK2<\/i>V617F allele burden in essential thrombocythemia"],"prefix":"10.1002","volume":"109","author":[{"given":"Jaya","family":"Kittur","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan A.","family":"Knudson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terra L.","family":"Lasho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christy 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