{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T09:22:08Z","timestamp":1774603328581,"version":"3.50.1"},"reference-count":49,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,5,16]],"date-time":"2007-05-16T00:00:00Z","timestamp":1179273600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer"],"published-print":{"date-parts":[[2007,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND.<\/jats:title><jats:p>Homologous deletion of the xeroderma pigmentosum complementary group C (<jats:italic>XPC<\/jats:italic>) repair gene frequently causes lung adenocarcinoma in mice, suggesting that an <jats:italic>XPC<\/jats:italic> defect may play a critical role in lung tumorigenesis. The current study attempted to determine whether reduced <jats:italic>XPC<\/jats:italic> mRNA levels predict the clinical outcome of lung cancer patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS.<\/jats:title><jats:p>XPC, p27<jats:sup>kip<\/jats:sup> (cdk inhibitory protein), and S\u2010phase kinase\u2010associated protein (skp2) levels were evaluated by Western blot analysis in a series of lung cancer cell lines with different invasive abilities. Migration and invasive abilities were measured using a modified Boyden chamber without and with Matrigel, respectively. To test whether XPC affects cell invasive ability, <jats:italic>XPC<\/jats:italic> gene protein expression was reduced in low invasive cells by RNA interference (RNAi) and assayed with Boyden chamber. <jats:italic>XPC<\/jats:italic> mRNA levels in 126 nonsmall cell lung cancers (NSCLCs) were examined by real\u2010time\u2013reverse\u2010transcriptase polymerase chain reaction (RT\u2010PCR). The prognostic value of <jats:italic>XPC<\/jats:italic> mRNA expression was statistically analyzed by the Kaplan\u2010Meier method and Cox proportional hazards regression.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS.<\/jats:title><jats:p>The expression of XPC was reduced with increasing invasive potential in CL1\u2010series lung cancer cell lines. When the XPC level was reduced by RNAi, cell migration and invasiveness increased markedly; the increased invasiveness may be caused by decreased expression of p27<jats:sup>kip<\/jats:sup> and increased expression of skp2 and E2F transcription factor 1. To determine whether reduced XPC expression was correlated with tumor aggressiveness and poor patient survival, <jats:italic>XPC<\/jats:italic> mRNA levels were evaluated by real\u2010time RT\u2010PCR. Kaplan\u2010Meier analysis demonstrated that the median survival of patients with lower <jats:italic>XPC<\/jats:italic> mRNA levels was shorter compared with patients with higher <jats:italic>XPC<\/jats:italic> mRNA levels (<jats:italic>P<\/jats:italic> = .0440). Cox regression analysis further indicated that <jats:italic>XPC<\/jats:italic> mRNA level may act as an independent prognostic factor for NSCLC patients (<jats:italic>P<\/jats:italic> = .014).<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS.<\/jats:title><jats:p>The results of the current study suggest that reduced <jats:italic>XPC<\/jats:italic> mRNA level may constitute an independent prognostic factor for NSCLC patients. Cancer 2007. \u00a9 2007 American Cancer Society.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncr.22743","type":"journal-article","created":{"date-parts":[[2007,5,16]],"date-time":"2007-05-16T18:49:02Z","timestamp":1179341342000},"page":"215-223","source":"Crossref","is-referenced-by-count":22,"title":["Reduced XPC messenger RNA level may predict a poor outcome of patients with nonsmall cell lung cancer"],"prefix":"10.1002","volume":"110","author":[{"given":"Yi\u2010Hui","family":"Wu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya\u2010Wen","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinghua Tsai","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tzu\u2010Chin","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chih\u2010Yi","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huei","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,6,15]]},"reference":[{"key":"e_1_2_6_2_2","volume-title":"Health Statistics, 2002","author":"Department of Health, Executive Yuan, Republic of China","year":"2002"},{"key":"e_1_2_6_3_2","volume-title":"Health Statistics, 2003","author":"Department of Health, Executive Yuan, Republic of China","year":"2003"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2006.07.0268"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2005.05.4163"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2005.08.409"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/em.1037"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1081\/GNC-120021371"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/35094000"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/92.21.1764"},{"key":"e_1_2_6_11_2","first-page":"4103","article-title":"Reduced DNA repair capacity in lung cancer patients","volume":"56","author":"Wei Q","year":"1996","journal-title":"Cancer Res."},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/88.19.1346"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2005.03.007"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh541"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cr.7290375"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2002.19628.x"},{"key":"e_1_2_6_17_2","first-page":"771","article-title":"Mutational inactivation of the xeroderma pigmentosum group C gene confers predisposition to 2\u2010acetylaminofluorene\u2010induced liver and lung cancer and to spontaneous testicular cancer in Trp53\u2212\/\u2212 mice","volume":"59","author":"Cheo DL","year":"1999","journal-title":"Cancer Res."},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0503133102"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.67.1.425"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7624798"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-579X(02)00098-6"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2004.01.035"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/93.18.1392"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2004.07.018"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209286"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0215(20000520)89:3<265::AID-IJC9>3.0.CO;2-N"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-3388"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-04-2308"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2005.08.127"},{"key":"e_1_2_6_30_2","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1158\/1078-0432.173.11.1","article-title":"Prognosis of non\u2010small cell lung cancer patients by detecting circulating cancer cells in the peripheral blood with multiple marker genes","volume":"11","author":"Sher YP","year":"2005","journal-title":"Clin Cancer Res."},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.20898"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.202485699"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.11346"},{"key":"e_1_2_6_34_2","first-page":"2799","article-title":"The association of human papillomavirus 16\/18 infection with lung cancer among nonsmoking Taiwanese women","volume":"61","author":"Cheng YW","year":"2001","journal-title":"Cancer Res."},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.20597"},{"key":"e_1_2_6_36_2","first-page":"140","article-title":"Chinese food cooking and lung cancer in Taiwan","volume":"51","author":"Ko YC","year":"2001","journal-title":"Am J Epidemiol."},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1021\/tx0000419"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.21087"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.16.9.4852"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1568-7864(03)00002-8"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1568-7864(03)00087-9"},{"key":"e_1_2_6_42_2","first-page":"4828","article-title":"Accumulation of p53 protein correlates with a poor prognosis in human lung cancer","volume":"52","author":"Quinlan DC","year":"1992","journal-title":"Cancer Res."},{"key":"e_1_2_6_43_2","first-page":"3381","article-title":"Prognostic role of the cyclin\u2010dependent kinase inhibitor p27 in non\u2010small cell lung cancer","volume":"57","author":"Esposito V","year":"1997","journal-title":"Cancer Res."},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-5002(01)00216-1"},{"key":"e_1_2_6_45_2","first-page":"1756","article-title":"Skp2 overexpression is a prognostic factor in patients with ovarian adenocarcinoma","volume":"9","author":"Shigemasa K","year":"2003","journal-title":"Clin Cancer Res."},{"key":"e_1_2_6_46_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(200004)183:1<10::AID-JCP2>3.0.CO;2-I"},{"key":"e_1_2_6_47_2","first-page":"7044","article-title":"High expression of S\u2010phase kinase\u2010interacting protein 2, human F\u2010box protein, correlates with poor prognosis in oral squamous cell carcinomas","volume":"61","author":"Kudo Y","year":"2001","journal-title":"Cancer Res."},{"key":"e_1_2_6_48_2","first-page":"3819","article-title":"Clinical and biological significance of S\u2010phase kinase\u2010associated protein 2 (Skp2) gene expression in gastric carcinoma: modulation of malignant phenotype by Skp2 overexpression, possibly via p27 proteolysis","volume":"62","author":"Masuda T","year":"2002","journal-title":"Cancer Res."},{"key":"e_1_2_6_49_2","first-page":"1756","article-title":"Skp2 overexpression is a prognostic factor in patients with ovarian adenocarcinoma","volume":"9","author":"Shigemasa K","year":"2003","journal-title":"Clin Cancer Res."},{"key":"e_1_2_6_50_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2004.01.035"}],"container-title":["Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcncr.22743","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/acsjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cncr.22743","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T11:22:50Z","timestamp":1760268170000},"score":1,"resource":{"primary":{"URL":"https:\/\/acsjournals.onlinelibrary.wiley.com\/doi\/10.1002\/cncr.22743"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,6,15]]},"references-count":49,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,7]]}},"alternative-id":["10.1002\/cncr.22743"],"URL":"https:\/\/doi.org\/10.1002\/cncr.22743","archive":["Portico"],"relation":{},"ISSN":["0008-543X","1097-0142"],"issn-type":[{"value":"0008-543X","type":"print"},{"value":"1097-0142","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,6,15]]}}}