{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T15:03:43Z","timestamp":1784127823482,"version":"3.55.0"},"reference-count":35,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2009,3,11]],"date-time":"2009-03-11T00:00:00Z","timestamp":1236729600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Ministero dell'Universit\u00e0 e della Ricerca (MIUR)"},{"name":"Progetti di Ricerca di Interesse Nazionale (PRIN)","award":["2004065502-004"],"award-info":[{"award-number":["2004065502-004"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer"],"published-print":{"date-parts":[[2009,5,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND:<\/jats:title><jats:p>Recent studies have suggested that <jats:italic>Helicobacter pylori<\/jats:italic> (<jats:italic>H. pylori<\/jats:italic>) infection may be related to better prognosis in patients with gastric cancer, but to the authors' knowledge, this finding has not yet been validated. In the current study, the association between <jats:italic>H. pylori<\/jats:italic> status and clinical outcome was investigated in a large cohort of patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS:<\/jats:title><jats:p>Frozen non\u2010neoplastic gastric mucosa and serum samples obtained from 297 patients who underwent surgery for primary gastric cancer between 1988 and 2004 were retrieved from the serum and tissue bank of the study department. <jats:italic>H. pylori<\/jats:italic> status was defined by means of polymerase chain reaction (PCR) analysis for the <jats:italic>vacA<\/jats:italic> gene in gastric mucosa and by serologic assay of <jats:italic>H. pylori<\/jats:italic> and CagA antibodies. Univariate and multivariate analyses were used for the association between clinicopathologic variables and long\u2010term outcome.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS:<\/jats:title><jats:p>Positivity for <jats:italic>H. pylori<\/jats:italic> infection was observed in 256 of 297 patients (86%), whereas in 41 patients (14%), PCR for <jats:italic>vacA<\/jats:italic> and both serologic tests were negative. Negative <jats:italic>H. pylori<\/jats:italic> status was found to be significantly associated with cardia location, advanced pT classification, noncurative surgery, and a lower 5\u2010year survival rate after R0 resection (24% vs 57%; <jats:italic>P<\/jats:italic> &lt; .001). Multivariate survival analysis confirmed <jats:italic>H. pylori<\/jats:italic> status as a significant prognostic factor (hazards ratio, 2.47; 95% confidence interval, 1.40\u20104.35 [<jats:italic>P<\/jats:italic> = .002]). The influence of <jats:italic>H. pylori<\/jats:italic> status on long\u2010term survival was observed in patients with early as well as advanced pT classifications.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS:<\/jats:title><jats:p>Negative <jats:italic>H. pylori<\/jats:italic> status appears to be an indicator of poor prognosis in patients with gastric cancer, and is independent of other well\u2012known clinical and pathologic prognostic variables. Cancer 2009. \u00a9 2009 American Cancer Society.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncr.24253","type":"journal-article","created":{"date-parts":[[2009,3,11]],"date-time":"2009-03-11T10:49:23Z","timestamp":1236768563000},"page":"2071-2080","source":"Crossref","is-referenced-by-count":90,"title":["Negative <i>Helicobacter pylori<\/i> status is associated with poor prognosis in patients with gastric 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