{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T19:47:01Z","timestamp":1779220021982,"version":"3.51.4"},"reference-count":36,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2009,7,22]],"date-time":"2009-07-22T00:00:00Z","timestamp":1248220800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer"],"published-print":{"date-parts":[[2009,10,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND:<\/jats:title><jats:p>Liver\u2010intestine cadherin (LI\u2010cadherin; CDH\u201017) is a new member of the cadherin superfamily with distinct structural and functional features. The study was designed to investigate the role of LI\u2010cadherin in tumor invasion and prognosis of human hepatitis B virus (HBV)\u2010positive hepatocellular carcinoma (HCC).<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS:<\/jats:title><jats:p>LI\u2010cadherin expression in HBV\u2010positive hepatocellular carcinoma cell lines with low\u2010 and high\u2010invasive potentials was evaluated by Western\u2010blot, immunofluorescence, and real\u2010time polymerase chain reaction (PCR) analyses. The role of LI\u2010cadherin in tumor invasion was also evaluated in vitro by a small\u2010interfering ribonucleic acid (siRNA)\u2010mediated approach. The prognostic significance of LI\u2010cadherin was validated in a cohort of HBV\u2010positive HCC patients by immunohistochemistry and Western\u2010blot.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS:<\/jats:title><jats:p>Significant high levels of LI\u2010cadherin mRNA and protein were found in the high\u2010invasive HCCLM3 as compared with those in low\u2010invasive PLC\/PRF\/5 and Hep3B cell line. Cell migration, adhesion to extracellular matrix, and matrigel invasion were significantly reduced after LI\u2010cadherin knockdown in HCCLM3 cells. Immunohistochemical analysis of 255 HBV\u2010positive HCC cases showed that overexpression of LI\u2010cadherin was well correlated with microvascular invasion, which was confirmed by Western\u2010blot in 32 tumor tissues, and its overexpression was strongly associated with shorter overall survival as well as higher incidence of tumor recurrence.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS:<\/jats:title><jats:p>LI\u2010cadherin is predictive of microvascular invasion and poor prognosis of HBV\u2010positive HCC, and would be a potential useful intervention target for HCC. Cancer 2009. \u00a9 2009 American Cancer Society.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncr.24513","type":"journal-article","created":{"date-parts":[[2009,7,22]],"date-time":"2009-07-22T10:05:22Z","timestamp":1248257122000},"page":"4753-4765","source":"Crossref","is-referenced-by-count":41,"title":["Liver\u2010intestine cadherin predicts microvascular invasion and poor prognosis of hepatitis B virus\u2010positive hepatocellular 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