{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T17:05:32Z","timestamp":1761843932522,"version":"build-2065373602"},"reference-count":14,"publisher":"Wiley","issue":"S4","license":[{"start":{"date-parts":[[2010,2,2]],"date-time":"2010-02-02T00:00:00Z","timestamp":1265068800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer"],"published-print":{"date-parts":[[2010,2,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND:<\/jats:title><jats:p>Antibody\u2010based imaging agents are available commercially, but their success has been limited, mainly because of low contrast and the emergence of 2\u2010fluoro\u20102\u2010deoxy\u2010D\u2010glucose\u2010positron emission tomography (FDG\u2010PET) scanning. In pretargeting, administration of the radionuclide is separated from the antibody, thereby enhancing image contrast and allowing detection at earlier time points after injection.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS:<\/jats:title><jats:p>The authors conducted an open\u2010label, single\u2010arm trial that assessed a pretargeting procedure in which an anticarcinoembryonic antigen x (anti\u2010CEA x) anti\u2010diethylenetriaminepentaacetic acid (anti\u2010DTPA)\u2010indum (In) antibody was used in combination with a <jats:sup>111<\/jats:sup>In\u2010labeled di\u2010DTPA peptide for the diagnostic imaging of CEA\u2010expressing colorectal cancer. Three patients received the <jats:sup>111<\/jats:sup>In peptide alone to investigate tumor targeting, organ distribution, and clearance of the peptide. Thereafter, 11 patients received the bispecific antibody (bsAb) (5 mg) to pretarget the tumor. After 3 to 5 days, patients were injected with 185 megabecquerels of <jats:sup>111<\/jats:sup>In\u2010labeled peptide to assess the optimal interval for best image quality.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS:<\/jats:title><jats:p>Fourteen patients with primary colorectal cancer were enrolled. One of 3 patients who received <jats:sup>111<\/jats:sup>In peptide alone had low\u2010level tumor uptake. In 9 of 11 other patients, tumors were observed. In 1 patient, FDG\u2010PET\u2013positive lymph nodes were observed clearly with pretargeted immunoscintigraphy. Peptide pharmacokinetics revealed enhanced circulating levels of <jats:sup>111<\/jats:sup>In\u2010labeled peptide in patients in the 3\u2010day interval cohort compared with the other cohorts. Tumor\u2010to\u2010background ratios ranged from 3.5 to 6.4 in the 3\u2010day interval group, from 5.1 to 14.2 in the 4\u2010day interval group, and from 3.5 to 3.9 in the 5\u2010day interval group. The best images were acquired with a 4\u2010day interval at 24 hours after injection of the radiolabeled peptide. Grade 1 adverse events were observed in 2 patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS:<\/jats:title><jats:p>Imaging of colorectal cancer using a 2\u2010step, pretargeting system produced the best imaging results 24 hours after peptide administration using a 4\u2010day interval between injection of the bsAb and the peptide. Cancer 2010;116(4 suppl):1111\u20137. \u00a9 2010 American Cancer Society.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncr.24799","type":"journal-article","created":{"date-parts":[[2010,2,2]],"date-time":"2010-02-02T14:54:36Z","timestamp":1265122476000},"page":"1111-1117","source":"Crossref","is-referenced-by-count":17,"title":["Pretargeted radioimmunoscintigraphy in patients with primary colorectal cancer using a bispecific anticarcinoembryonic antigen CEA X anti\u2010di\u2010diethylenetriaminepentaacetic acid F(ab\u2032)<sub>2<\/sub> antibody"],"prefix":"10.1002","volume":"116","author":[{"given":"Frits","family":"Aarts","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Otto C.","family":"Boerman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert M.","family":"Sharkey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thijs","family":"Hendriks","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chien\u2010Hsing","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William J.","family":"McBride","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert P.","family":"Bleichrodt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wim J. G.","family":"Oyen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David M.","family":"Goldenberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,2,2]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"1662","article-title":"Bispecific monoclonal antibody\u2010mediated targeting of an indium\u2010111\u2010labeled DTPA dimer to primary colorectal tumors: pharmacokinetics, biodistribution, scintigraphy and immune response","volume":"34","author":"Le Doussal JM","year":"1993","journal-title":"J Nucl Med"},{"key":"e_1_2_6_3_2","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1016\/0016-5085(83)90229-9","article-title":"Carcinoembryonic antigen radioimmunodetection in colorectal cancer [letter]","volume":"84","author":"Goldenberg DM","year":"1983","journal-title":"Gastroenterology"},{"key":"e_1_2_6_4_2","first-page":"5786s","article-title":"Diagnostic accuracy and tumor\u2010targeting kinetics of complete versus fragmented 99mTc\u2010labeled anti\u2010carcinoembryonic antigen antibodies: an intraindividual comparison","volume":"55","author":"Behr TM","year":"1995","journal-title":"Cancer Res"},{"key":"e_1_2_6_5_2","first-page":"3973s","article-title":"Pharmacokinetics and dosimetry studies for optimization of anti\u2010carcinoembryonic antigen x anti\u2010hapten bispecific antibody\u2010mediated pretargeting of iodine\u2010131\u2010labeled hapten in a phase I radioimmunotherapy trial","volume":"9","author":"Kraeber\u2010Bodere F","year":"2003","journal-title":"Clin Cancer Res"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-1004-0009"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006231-199412000-00009"},{"key":"e_1_2_6_8_2","first-page":"247","article-title":"Targeting, toxicity, and efficacy of 2\u2010step, pretargeted radioimmunotherapy using a chimeric bispecific antibody and 131I\u2010labeled bivalent hapten in a phase I optimization clinical trial","volume":"47","author":"Kraeber\u2010Bodere F","year":"2006","journal-title":"J Nucl Med"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2005.04.4917"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-1004-0010"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00259-006-0226-z"},{"key":"e_1_2_6_12_2","first-page":"1172","article-title":"Radioimmunodetection of medullary thyroid carcinoma using indium\u2010111 bivalent hapten and anti\u2010CEA x anti\u2010DTPA\u2010indium bispecific antibody","volume":"39","author":"Barbet J","year":"1998","journal-title":"J Nucl Med"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.uct.2007.04.003"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.semnuclmed.2005.12.002"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2007-0938"}],"container-title":["Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcncr.24799","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/acsjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cncr.24799","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T15:27:23Z","timestamp":1760282843000},"score":1,"resource":{"primary":{"URL":"https:\/\/acsjournals.onlinelibrary.wiley.com\/doi\/10.1002\/cncr.24799"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,2,2]]},"references-count":14,"journal-issue":{"issue":"S4","published-print":{"date-parts":[[2010,2,15]]}},"alternative-id":["10.1002\/cncr.24799"],"URL":"https:\/\/doi.org\/10.1002\/cncr.24799","archive":["Portico"],"relation":{},"ISSN":["0008-543X","1097-0142"],"issn-type":[{"type":"print","value":"0008-543X"},{"type":"electronic","value":"1097-0142"}],"subject":[],"published":{"date-parts":[[2010,2,2]]}}}