{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:37:03Z","timestamp":1786977423514,"version":"build-2736575974"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2010,1,5]],"date-time":"2010-01-05T00:00:00Z","timestamp":1262649600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer"],"published-print":{"date-parts":[[2010,2,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND:<\/jats:title>\n                    <jats:p>Epstein\u2010Barr virus (EBV) infection has been associated with Richter transformation in patients with chronic lymphocytic leukemia (CLL).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS:<\/jats:title>\n                    <jats:p>A direct isothermal mRNA amplification method was developed for detection of EBV latent membrane protein 1 (LMP1) mRNA transcriptional activity in the peripheral blood of 135 chronic lymphocytic leukemia patients and 98 hematologically healthy control subjects.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS:<\/jats:title>\n                    <jats:p>\n                      EBV LMP1 mRNA transcripts were found in 19 of 135 (14%) of the CLL cases, but only 1% of the healthy controls (\n                      <jats:italic>P<\/jats:italic>\n                      &lt; .0001). In contrast, 23 solid tumor patients tested negative for EBV LMP1 transcripts. In a later cohort of patients after hematopoietic stem cell transplantation, 4 of 7 patients with Hodgkin lymphoma or Burkitt lymphoma had EBV LMP1 detected. In a preliminary analysis, outcome data were available for 88 of the 135 patients with CLL. EBV LMP1 mRNA positivity was associated with a significantly increased degree of histologically demonstrated bone marrow involvement by CLL (\n                      <jats:italic>P<\/jats:italic>\n                      = .003, Mann\u2010Whitney\n                      <jats:italic>U<\/jats:italic>\n                      test).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS:<\/jats:title>\n                    <jats:p>\n                      EBV LMP1 mRNA transcriptional activity was observed in a significant proportion of CLL patients. Transcription of the EBV\n                      <jats:italic>LMP1<\/jats:italic>\n                      , a late gene with known transforming potential in vitro, suggests that EBV activation plays a role in CLL disease progression. Thus, EBV LMP1 expression in CLL patients may be a factor involved in the genesis of refractory disease. Cancer 2010. \u00a9 2010 American Cancer Society.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cncr.24839","type":"journal-article","created":{"date-parts":[[2010,1,5]],"date-time":"2010-01-05T10:43:38Z","timestamp":1262688218000},"page":"880-887","source":"Crossref","is-referenced-by-count":24,"title":["Epstein\u2010Barr virus latent membrane protein 1 mRNA is expressed in a significant proportion of patients with chronic lymphocytic leukemia"],"prefix":"10.1002","volume":"116","author":[{"given":"Jeffrey J.","family":"Tarrand","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Keating","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Apostolia M.","family":"Tsimberidou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"O'Brien","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rocco P.","family":"LaSala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang\u2010Yang","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos E.","family":"Bueso\u2010Ramos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,1,5]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V87.12.4990.bloodjournal87124990"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(99)90089-8"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI23409"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2006.040956"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1080\/10428190500398856"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.seminoncol.2006.01.016"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1080\/10245330410001727046"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-8652(199902)60:2<99::AID-AJH3>3.0.CO;2-T"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.22121"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2008.02.019"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.4.1800-1816.2004"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1309\/PPK0-TJUK-1UAR-3194"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.28.3.495-503.1990"},{"key":"e_1_2_7_15_2","doi-asserted-by":"crossref","first-page":"3164","DOI":"10.1128\/JCM.36.11.3164-3169.1998","article-title":"Nucleic acid sequence\u2010based amplification of spliced and unspliced Epstein\u2010Barr latent transcripts","volume":"36","author":"Brink AA","year":"1998","journal-title":"J Clin Microbiol"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.19.9078-9082.2000"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2004.10.011"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2008.08.002"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1385\/MB:20:2:163"},{"key":"e_1_2_7_20_2","unstructured":"KirkmanTW. 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