{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T12:22:25Z","timestamp":1790166145729,"version":"4.1.0"},"reference-count":53,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2010,6,8]],"date-time":"2010-06-08T00:00:00Z","timestamp":1275955200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer"],"published-print":{"date-parts":[[2010,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND:<\/jats:title><jats:p>DNA methylation may be used a potential biomarker for detecting cervical cancer. The authors of this report used quantitative methylation analysis of 4 genes in a full spectrum of cervical lesions to test its potential clinical application.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS:<\/jats:title><jats:p>This hospital\u2010based, retrospective, case\u2010control study was conducted in 185 patients and included patients who had a normal uterine cervix (n = 53), cervical intraepithelial neoplasm type 1 (CIN1) (n = 37), CIN2 (n = 22), CIN3 (n = 24), carcinoma in situ (CIS) (n = 22), squamous cell carcinoma (SCC, n = 20), and adenocarcinoma (AC) (n = 7). Methylation levels of the genes sex\u2010determining region Y, box 1 (<jats:italic>SOX1<\/jats:italic>); paired box gene 1 (<jats:italic>PAX1<\/jats:italic>); LIM homeobox transcription factor 1\u03b1 (<jats:italic>LMX1A<\/jats:italic>), and NK6 transcription factor\u2010related locus 1 (<jats:italic>NKX6\u20101<\/jats:italic>) were determined by using real\u2010time methylation\u2010specific polymerase chain reaction (PCR) amplification. Cutoff values of the percentage of methylation reference (PMR) for different diagnoses were determined to test the sensitivity and specificity and to generate receiver operating characteristic (ROC) curves. Two\u2010sided Mann\u2010Whitney <jats:italic>U<\/jats:italic> tests were used to test differences in PMR between groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS:<\/jats:title><jats:p>The PMRs of the 4 genes were significantly higher in CIN3 and worse (CIN3+) lesions than the PMRs in specimens of normal cervix and CIN1 or CIN2 (<jats:italic>P<\/jats:italic> &lt; .001). ROC curve analysis demonstrated that the sensitivity, specificity, and accuracy for detecting CIN3+ lesions were 0.88, 0.82, and 0.95, respectively, for <jats:italic>SOX1<\/jats:italic>; 0.78, 0.91, and 0.89, respectively, for <jats:italic>PAX1<\/jats:italic>; 0.77, 0.88, and 0.90, respectively, for <jats:italic>LMX1A<\/jats:italic>; and 0.93, 0.97, and 0.97, respectively, for <jats:italic>NKX6\u20101<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS:<\/jats:title><jats:p>The current results indicated that quantitative PCR\u2010based testing for DNA methylation of 4 genes holds great promise for cervical cancer screening and warrants further population\u2010based studies using standardized DNA methylation testing. 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