{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T09:18:48Z","timestamp":1776503928474,"version":"3.51.2"},"reference-count":50,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2011,3,29]],"date-time":"2011-03-29T00:00:00Z","timestamp":1301356800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer"],"published-print":{"date-parts":[[2011,10,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND:<\/jats:title><jats:p>The objective of this study was to link expression patterns of B\u2010cell\u2013specific Moloney murine leukemia virus integration site 1 (Bmi\u20101) and p16 to patient outcome (recurrence and survival) in a cohort of 252 patients with oral and oropharyngeal squamous cell cancer (OSCC).<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS:<\/jats:title><jats:p>Expression levels of Bmi\u20101 and p16 in samples from 252 patients with OSCC were evaluated immunohistochemically using the tissue microarray method. Staining intensity was determined by calculating an intensity reactivity score (IRS). Staining intensity and the localization of expression within tumor cells (nuclear or cytoplasmic) were correlated with overall, disease\u2010specific, and recurrence\u2010free survival.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS:<\/jats:title><jats:p>The majority of cancers were localized in the oropharynx (61.1%). In univariate analysis, patients who had OSCC and strong Bmi\u20101 expression (IRS &gt;10) had worse outcomes compared with patients who had low and moderate Bmi\u20101 expression (<jats:italic>P<\/jats:italic> = .008; hazard ratio [HR], 1.82; 95% confidence interval [CI], 1.167\u20102.838); this correlation was also observed for atypical cytoplasmic Bmi\u20101 expression (<jats:italic>P<\/jats:italic> = .001; HR, 2.164; 95% CI, 1.389\u20103.371) and for negative p16 expression (<jats:italic>P<\/jats:italic> &lt; .001; HR, 0.292; 95% CI, 0.178\u20100.477). The combination of both markers, as anticipated, had an even stronger correlation with overall survival (<jats:italic>P<\/jats:italic> &lt; .001; HR, 8.485; 95% CI, 4.237\u201016.994). Multivariate analysis demonstrated significant results for patients with oropharyngeal cancers, but not for patients with oral cavity tumors: Tumor classification (<jats:italic>P<\/jats:italic> = .011; HR, 1.838; 95%CI, 1.146\u20102.947) and the combined marker expression patterns (<jats:italic>P<\/jats:italic> &lt; .001; HR, 6.254; 95% CI, 2.869\u201013.635) were correlated with overall survival, disease\u2010specific survival (tumor classification: <jats:italic>P<\/jats:italic> = .002; HR, 2.807; 95% CI, 1.477\u20105.334; combined markers: <jats:italic>P<\/jats:italic> = .002; HR, 5.386; 95% CI, 1.850\u201015.679), and the combined markers also were correlated with recurrence\u2010free survival (<jats:italic>P<\/jats:italic> = .001; HR, 8.943; 95% CI, 2.562\u201031.220).<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS:<\/jats:title><jats:p>Cytoplasmic Bmi\u20101 expression, an absence of p16 expression, and especially the combination of those 2 predictive markers were correlated negatively with disease\u2010specific and recurrence\u2010free survival in patients with oropharyngeal cancer. Therefore, the current results indicate that these may be applicable as predictive markers in combination with other factors to select patients for more aggressive treatment and follow\u2010up. Cancer 2011;. \u00a9 2011 American Cancer Society.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncr.26100","type":"journal-article","created":{"date-parts":[[2011,3,29]],"date-time":"2011-03-29T10:31:43Z","timestamp":1301394703000},"page":"4659-4670","source":"Crossref","is-referenced-by-count":54,"title":["Expression patterns of Bmi\u20101 and p16 significantly correlate with overall, disease\u2010specific, and recurrence\u2010free survival in oropharyngeal squamous cell carcinoma"],"prefix":"10.1002","volume":"117","author":[{"given":"Gerhard 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