{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T12:46:18Z","timestamp":1790340378236,"version":"4.1.0"},"reference-count":19,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2014,6,12]],"date-time":"2014-06-12T00:00:00Z","timestamp":1402531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Medical Reseach Council, Singapore","award":["NMRC\/0697\/2002"],"award-info":[{"award-number":["NMRC\/0697\/2002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer"],"published-print":{"date-parts":[[2014,10,15]]},"abstract":"<jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>Serum carcinoembryonic antigen (CEA) is the only marker recommended for surveillance of colorectal cancer (CRC) recurrence; its sensitivity and specificity, however, are suboptimal. This study sought to evaluate the values of postoperative serum methylation levels of 7 genes for prognostication and especially for recurrence detection after curative resection.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>This prospective cohort study included 150 patients with stage I\u2010III CRC from whom 3 consecutive blood sampling was taken 1 week before, and 6 months and 1 year after operation. Methylation levels of 7 genes were evaluated via quantitative methylation\u2010specific polymerase chain reaction. Serum CEA was measured in parallel. Univariate and multivariate survival analyses were followed by construction of receiver operating characteristic curves for recurrence detection.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      After a median follow\u2010up of 59 months, 43 patients (28.7%) developed recurrent lesions. High serum methylation levels of\n                      <jats:italic>TAC1<\/jats:italic>\n                      in serum at 6\u2010month follow\u2010up (6M\u2010FU), and\n                      <jats:italic>SEPT9<\/jats:italic>\n                      at 1\u2010year follow\u2010up (1Y\u2010FU) were independent predictors for tumor recurrence and unfavorable cancer\u2010specific survival (CSS) (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u2009.05 in all tests). Serum\n                      <jats:italic>NELL1<\/jats:italic>\n                      methylation levels were significant alone for CSS at both 6M\u2010FU and 1Y\u2010FU, but not for disease\u2010free survival. Dynamic changes of\n                      <jats:italic>TAC1<\/jats:italic>\n                      and\n                      <jats:italic>SEPT9<\/jats:italic>\n                      with methylation increment were also independently predictive for recurrence (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u2009.05 in all tests). More importantly,\n                      <jats:italic>TAC1<\/jats:italic>\n                      at 6M\u2010FU and\n                      <jats:italic>SEPT9<\/jats:italic>\n                      at 1Y\u2010FU exhibited earlier detection of potential recurrences compared with concurrent serum CEA.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS<\/jats:title>\n                    <jats:p>\n                      Levels of\n                      <jats:italic>TAC1<\/jats:italic>\n                      and\n                      <jats:italic>SEPT9<\/jats:italic>\n                      methylation detected in postoperative sera of patients with CRC appear to be novel promising prognostic markers and may probably be considered for monitoring of CRC recurrence.\n                      <jats:bold>\n                        <jats:italic>Cancer<\/jats:italic>\n                        2014;120:3131\u20133141.\n                      <\/jats:bold>\n                      \u00a9\n                      <jats:italic>2014 American Cancer Society<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cncr.28802","type":"journal-article","created":{"date-parts":[[2014,6,12]],"date-time":"2014-06-12T11:12:11Z","timestamp":1402571531000},"page":"3131-3141","source":"Crossref","is-referenced-by-count":85,"title":["Postoperative serum methylation levels of\n                    <i>TAC1<\/i>\n                    and\n                    <i>SEPT9<\/i>\n                    are independent predictors of recurrence and survival of patients with colorectal cancer"],"prefix":"10.1002","volume":"120","author":[{"given":"CheeKian","family":"Tham","sequence":"first","affiliation":[{"name":"Department of Medical Oncology National Cancer Centre of Singapore  Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MinHoe","family":"Chew","sequence":"additional","affiliation":[{"name":"Department of Colorectal Surgery Singapore General Hospital  Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richie","family":"Soong","sequence":"additional","affiliation":[{"name":"Cancer Science Institute of Singapore National University of Singapore  Singapore"},{"name":"Department of Pathology National University Health System  Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JitFong","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Colorectal Surgery Singapore General Hospital  Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MeiKim","family":"Ang","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology National Cancer Centre of Singapore  Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ChoongLeong","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Colorectal Surgery Singapore General Hospital  Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Clinical Research Singapore General Hospital  Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon Y. K.","family":"Ong","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology National Cancer Centre of Singapore  Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanqun","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Colorectal Surgery Singapore General Hospital  Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,6,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2008.19.5362"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/DCR.0b013e31820db364"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02239433"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1001\/archsurg.2010.251"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s003840050188"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/47.4.624"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6605651"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2008.05.005"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2010.10.002"},{"key":"e_1_2_7_11_1","unstructured":"National Comprehensive Cancer Network.NCCN Clinical Practice Guidelines in Oncology Colon Cancer. Version 2.2014.http:\/\/www.nccn.org\/professionals\/physician_gls\/f_guidelines.asp#site. 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