{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T04:43:33Z","timestamp":1789879413768,"version":"4.0.1"},"reference-count":27,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2017,9,26]],"date-time":"2017-09-26T00:00:00Z","timestamp":1506384000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 CA206517"],"award-info":[{"award-number":["R01 CA206517"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P50 CA097190"],"award-info":[{"award-number":["P50 CA097190"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["T32 CA060397"],"award-info":[{"award-number":["T32 CA060397"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer"],"published-print":{"date-parts":[[2017,11,15]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Human papillomavirus 16 (HPV16) E6 antibodies may be an early marker of the diagnosis and recurrence of human papillomavirus\u2013driven oropharyngeal cancer (HPV\u2010OPC).<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>This study identified 161 incident oropharyngeal cancer (OPC) cases diagnosed at the University of Pittsburgh (2003\u20102013) with pretreatment serum. One hundred twelve had preexisting clinical HPV testing with p16 immunohistochemistry and HPV in situ hybridization (87 were dual\u2010positive [HPV\u2010OPC], and 25 were dual\u2010negative [HPV\u2010negative]); 62 had at least 1 posttreatment serum sample. Eighty\u2010six of the 161 tumors were available for additional HPV16 DNA\/RNA testing (45 were dual\u2010positive [HPV16\u2013OPC], and 19 were dual\u2010negative [HPV16\u2013negative). HPV16 E6 antibody testing was conducted with multiplex serology. The following were evaluated: 1) the sensitivity and specificity of HPV16 E6 serology for distinguishing HPV\u2010OPC and HPV16\u2013OPC from HPV\u2010negative OPC, 2) HPV16 E6 antibody decay after treatment with linear models accommodating correlations in variance estimates, and 3) pre\u2010 and posttreatment HPV16 E6 levels and the risk of recurrence with Cox proportional hazards models.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Seventy\u2010eight of 87 HPV\u2010OPCs were HPV16 E6\u2013seropositive (sensitivity, 89.7%; 95% confidence interval [CI], 81.3%\u201095.2%), and 24 of 25 HPV\u2010negative OPCs were HPV16 E6\u2013seronegative (specificity, 96.0%; 95% CI, 79.6%\u201099.9%). Forty\u2010two of 45 HPV16\u2013OPCs were HPV16 E6\u2013seropositive (sensitivity, 93.3%; 95% CI, 81.7%\u201098.6%), and 18 of 19 HPV16\u2013negative OPCs were HPV16 E6\u2013seronegative (specificity, 94.7%; 95% CI, 74.0%\u201099.9%). Posttreatment HPV16 E6 antibody levels did not decrease significantly from the baseline (<jats:italic>P<\/jats:italic> = .575; median follow\u2010up, 307 days) and were not associated with the risk of recurrence. However, pretreatment HPV16 E6 seropositivity was associated with an 86% reduced risk of local\/regional recurrence (hazard ratio, 0.14; 95% CI, 0.03\u20100.68; <jats:italic>P<\/jats:italic> = .015).<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>HPV16 E6 antibodies may have potential clinical utility for the diagnosis and\/or prognosis of HPV\u2010OPC. <jats:bold><jats:italic>Cancer<\/jats:italic> 2017;123:4382\u201090<\/jats:bold>. \u00a9 <jats:italic>2017 American Cancer Society<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncr.30966","type":"journal-article","created":{"date-parts":[[2017,9,26]],"date-time":"2017-09-26T12:43:15Z","timestamp":1506429795000},"page":"4382-4390","source":"Crossref","is-referenced-by-count":85,"title":["Human papillomavirus 16 <scp>E<\/scp>6 antibodies are sensitive for human papillomavirus\u2013driven oropharyngeal cancer and are associated with recurrence"],"prefix":"10.1002","volume":"123","author":[{"given":"Krystle A.","family":"Lang Kuhs","sequence":"first","affiliation":[{"name":"National Cancer Institute National Institutes of Health Bethesda Maryland"},{"name":"Department of Medicine Vanderbilt University Medical Center Nashville Tennessee"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aim\u00e9e R.","family":"Kreimer","sequence":"additional","affiliation":[{"name":"National Cancer Institute National Institutes of Health Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumita","family":"Trivedi","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology and Cancer Immunology Program University of Pittsburgh Medical Center Hillman Cancer Center Pittsburgh Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dana","family":"Holzinger","sequence":"additional","affiliation":[{"name":"Division of Molecular Diagnostics of Oncogenic Infections German Cancer Research Center Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Pawlita","sequence":"additional","affiliation":[{"name":"Division of Molecular Diagnostics of Oncogenic Infections German Cancer Research Center Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth M.","family":"Pfeiffer","sequence":"additional","affiliation":[{"name":"National Cancer Institute National Institutes of Health Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra P.","family":"Gibson","sequence":"additional","affiliation":[{"name":"University of Pittsburgh Medical Center Hillman Cancer Center Pittsburgh Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole C.","family":"Schmitt","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Johns Hopkins University Baltimore Maryland"},{"name":"National Institute on Deafness and Other Communication Disorders National Institutes of Health Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allan","family":"Hildesheim","sequence":"additional","affiliation":[{"name":"National Cancer Institute National Institutes of Health Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tim","family":"Waterboer","sequence":"additional","affiliation":[{"name":"Division of Molecular Diagnostics of Oncogenic Infections German Cancer Research Center Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6605-2071","authenticated-orcid":false,"given":"Robert L.","family":"Ferris","sequence":"additional","affiliation":[{"name":"University of Pittsburgh Medical Center Hillman Cancer Center Pittsburgh 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