{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T03:05:05Z","timestamp":1791515105051,"version":"4.3.4"},"reference-count":22,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,11,19]],"date-time":"2019-11-19T00:00:00Z","timestamp":1574121600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007313","name":"University of Texas MD Anderson Cancer Center","doi-asserted-by":"publisher","award":["CA016672"],"award-info":[{"award-number":["CA016672"]}],"id":[{"id":"10.13039\/100007313","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer"],"published-print":{"date-parts":[[2020,2,15]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      The impact of the allelic burden of\n                      <jats:italic>ASXL1, DNMT3A, JAK2, TET2,<\/jats:italic>\n                      and\n                      <jats:italic>TP53<\/jats:italic>\n                      mutations on survival remains unclear in patients with newly diagnosed acute myeloid leukemia (AML).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The authors assessed bone marrow aspirates from 421 patients with newly diagnosed AML using next\u2010generation sequencing for\n                      <jats:italic>ASXL1, DNMT3A, JAK2, TET2,<\/jats:italic>\n                      and\n                      <jats:italic>TP53<\/jats:italic>\n                      mutations, defined as the presence of mutations in\n                      <jats:italic>ASXL1<\/jats:italic>\n                      ,\n                      <jats:italic>DNMT3A<\/jats:italic>\n                      ,\n                      <jats:italic>JAK2<\/jats:italic>\n                      ,\n                      <jats:italic>TET2<\/jats:italic>\n                      , or\n                      <jats:italic>TP53<\/jats:italic>\n                      with a minimum variant allele frequency (VAF) of 5%.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 71 patients (17%) had\n                      <jats:italic>ASXL1<\/jats:italic>\n                      mutations, 104 patients (25%) had\n                      <jats:italic>DNMT3A<\/jats:italic>\n                      mutations, 16 patients (4%) had\n                      <jats:italic>JAK2<\/jats:italic>\n                      mutations, 82 patients (20%) had\n                      <jats:italic>TET2<\/jats:italic>\n                      mutations, and 86 patients (20%) had\n                      <jats:italic>TP53<\/jats:italic>\n                      mutations. Among patients with each mutation, the median VAF of\n                      <jats:italic>ASXL1<\/jats:italic>\n                      was 34.31% (range, 1.17%\u201058.62%), the median VAF of\n                      <jats:italic>DNMT3A<\/jats:italic>\n                      was 41.76% (range, 1.02%\u201091.66%), the median VAF of\n                      <jats:italic>JAK2<\/jats:italic>\n                      was 46.70% (range, 10.4%\u201071.7%), the median VAF of\n                      <jats:italic>TET2<\/jats:italic>\n                      was 42.78% (range, 2.26%\u201095.32%), and the median VAF of\n                      <jats:italic>TP53<\/jats:italic>\n                      was 45.47% (range, 1.15%\u201093.74%). The composite complete response rate was 60%, and was 77% in patients with AML with and without\n                      <jats:italic>ASXL1<\/jats:italic>\n                      ,\n                      <jats:italic>DNMT3A<\/jats:italic>\n                      ,\n                      <jats:italic>JAK2<\/jats:italic>\n                      ,\n                      <jats:italic>TET2<\/jats:italic>\n                      , or\n                      <jats:italic>TP53<\/jats:italic>\n                      mutations, respectively (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a0.006); the median overall survival was 11\u00a0months and 27\u00a0months, respectively (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a0.001). Multivariate analysis identified age; an antecedent history of dysplasia; white blood cell count; adverse cytogenetic risk; previous treatment with an\n                      <jats:italic>FLT3<\/jats:italic>\n                      inhibitor; and the VAF of\n                      <jats:italic>ASXL1, DNMT3A, JAK2, TET2, TP53,<\/jats:italic>\n                      and\n                      <jats:italic>NPM1<\/jats:italic>\n                      mutations by next\u2010generation sequencing as prognostic factors for overall survival.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The VAF of\n                      <jats:italic>ASXL1, DNMT3A, JAK2, TET2, TP53,<\/jats:italic>\n                      and\n                      <jats:italic>NPM1<\/jats:italic>\n                      mutations is associated with worse prognosis in patients with newly diagnosed AML.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cncr.32566","type":"journal-article","created":{"date-parts":[[2019,11,19]],"date-time":"2019-11-19T12:26:42Z","timestamp":1574166402000},"page":"765-774","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":94,"title":["Impact of the variant allele frequency of\n                    <i>ASXL1<\/i>\n                    ,\n                    <i>DNMT3A<\/i>\n                    ,\n                    <i>JAK2<\/i>\n                    ,\n                    <i>TET2<\/i>\n                    ,\n                    <i>TP53<\/i>\n                    , and\n                    <i>NPM1<\/i>\n                    on the outcomes of patients with newly diagnosed acute myeloid leukemia"],"prefix":"10.1002","volume":"126","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9140-0610","authenticated-orcid":false,"given":"Koji","family":"Sasaki","sequence":"first","affiliation":[{"name":"Department of Leukemia The University of Texas MD Anderson Cancer Center Houston Texas"},{"name":"Department of Hematology Graduate School of Medical and Dental Sciences Tokyo Medical and Dental University Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7829-5249","authenticated-orcid":false,"given":"Rashmi","family":"Kanagal\u2010Shamanna","sequence":"additional","affiliation":[{"name":"Department of Hematopathology The University of Texas MD Anderson Cancer Center Houston Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillermo","family":"Montalban\u2010Bravo","sequence":"additional","affiliation":[{"name":"Department of Leukemia The University of Texas MD Anderson Cancer Center Houston 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