{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:38:25Z","timestamp":1786977505852,"version":"build-2736575974"},"reference-count":43,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2022,9,15]],"date-time":"2022-09-15T00:00:00Z","timestamp":1663200000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Genomic landscape of extramedullary acute myeloid leukemia (EM\u2010AML), including myeloid sarcoma (MS) and leukemia cutis (LC), is not well characterized. The potential utility of next\u2010generation sequencing (NGS) using EM tissue is not established.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In this multicenter retrospective study, clinical and NGS data were collected on patients with EM\u2010AML. All statistical analyses were performed in SPSS Statistics (v 26).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Our study included 58 patients with EM\u2010AML. The median age at diagnosis was 62\u2009years; 59% of patients had MS and 33% had LC. EM\u2010AML was isolated (i.e., without blood or marrow involvement) in 31% and was first noted at relapse in 60% of patients. Median overall survival in our cohort was 18.2\u2009months overall, with 19.1\u2009months and 11.6\u2009months in the newly diagnosed and the relapsed\/refractory patients, respectively. At least one targetable or potentially targetable alteration was present in 52% of patients with EM\u2010site NGS, with 26%\n                      <jats:italic>IDH1<\/jats:italic>\n                      , 21%\n                      <jats:italic>NPM1<\/jats:italic>\n                      , 11%\n                      <jats:italic>IDH2<\/jats:italic>\n                      , 6%\n                      <jats:italic>FLT3<\/jats:italic>\n                      , and 13%\n                      <jats:italic>KMT2A\u2010PTD<\/jats:italic>\n                      . Mutations in\n                      <jats:italic>IDH1<\/jats:italic>\n                      were significantly more prevalent on NGS from EM tissue than non\u2010EM (blood or marrow) samples (26% vs. 3%;\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u2009.030). Three of four patients treated with IDH inhibitors based on EM\u2010site NGS experienced a complete response.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Targetable mutations are frequent in EM\u2010AML and EM\u2010site NGS is warranted for selecting potential targeted therapies for patients with EM\u2010AML.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cncr.34459","type":"journal-article","created":{"date-parts":[[2022,9,15]],"date-time":"2022-09-15T12:05:00Z","timestamp":1663243500000},"page":"3880-3887","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Molecular annotation of extramedullary acute myeloid leukemia identifies high prevalence of targetable mutations"],"prefix":"10.1002","volume":"128","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1754-1204","authenticated-orcid":false,"given":"Somedeb","family":"Ball","sequence":"first","affiliation":[{"name":"Division of Hematology and Medical Oncology H. Lee Moffitt Cancer Center and Research Institute  Tampa Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Todd C.","family":"Knepper","sequence":"additional","affiliation":[{"name":"Department of Individualized Cancer Management H. Lee Moffitt Cancer Center and Research Institute  Tampa Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yehuda E.","family":"Deutsch","sequence":"additional","affiliation":[{"name":"Department of Malignant Hematology and Cellular Therapy at Memorial Healthcare System Moffitt Cancer Center  Pembroke Pines Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wassim","family":"Samra","sequence":"additional","affiliation":[{"name":"Sylvester Comprehensive Cancer Center University of Miami  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin M.","family":"Watts","sequence":"additional","affiliation":[{"name":"Sylvester Comprehensive Cancer Center University of Miami  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terrence J.","family":"Bradley","sequence":"additional","affiliation":[{"name":"Sylvester Comprehensive Cancer Center University of Miami  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Onyee","family":"Chan","sequence":"additional","affiliation":[{"name":"Department of Malignant Hematology H. Lee Moffitt Cancer Center and Research Institute  Tampa Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Omar","family":"Hussaini","sequence":"additional","affiliation":[{"name":"Department of Hematopathology H. Lee Moffitt Cancer Center and Research Institute  Tampa Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Hematopathology H. Lee Moffitt Cancer Center and Research Institute  Tampa Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kendra L.","family":"Sweet","sequence":"additional","affiliation":[{"name":"Department of Malignant Hematology H. Lee Moffitt Cancer Center and Research Institute  Tampa Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9040-7415","authenticated-orcid":false,"given":"Andrew T.","family":"Kuykendall","sequence":"additional","affiliation":[{"name":"Department of Malignant Hematology H. Lee Moffitt Cancer Center and Research Institute  Tampa Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chetasi","family":"Talati","sequence":"additional","affiliation":[{"name":"Abbvie Inc. (formerly affiliated to Department of Malignant Hematology, H. Lee Moffitt Cancer Center, Tampa, Florida, USA)  North Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Padron","sequence":"additional","affiliation":[{"name":"Department of Malignant Hematology H. Lee Moffitt Cancer Center and Research Institute  Tampa Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rami S.","family":"Komrokji","sequence":"additional","affiliation":[{"name":"Department of Malignant Hematology H. Lee Moffitt Cancer Center and Research Institute  Tampa Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey E.","family":"Lancet","sequence":"additional","affiliation":[{"name":"Department of Malignant Hematology H. Lee Moffitt Cancer Center and Research Institute  Tampa Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Sallman","sequence":"additional","affiliation":[{"name":"Department of Malignant Hematology H. Lee Moffitt Cancer Center and Research Institute  Tampa Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,9,15]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-04-347229"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm4051102"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1746-1596-2-42"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1309\/WYACYWF6NGM3WBRT"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(197304)31:4<948::AID-CNCR2820310428>3.0.CO;2-N"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19810915)48:6<1426::AID-CNCR2820480626>3.0.CO;2-G"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-001-0412-9"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2019.223032"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/2040620711410774"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2230.2005.02022.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/10428190600967196"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.23691"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1159\/000253592"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402958"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/10245332.2017.1333275"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2012971"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2020004856"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1516192"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2004.05124.x"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.24072"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejh.13056"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1997.15.2.466"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.3171\/jns.2006.105.6.916"},{"key":"e_1_2_7_25_1","first-page":"19","article-title":"Myeloid sarcoma in the central nervous system: case report and review of the literature","volume":"47","author":"Struhal W","year":"2008","journal-title":"Acta Clin Croat"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19890601)63:11<2192::AID-CNCR2820631122>3.0.CO;2-R"},{"key":"e_1_2_7_27_1","first-page":"223","article-title":"Clinical, histopathologic, and immunohistochemical correlations in leukemia cutis","volume":"13","author":"Su WP","year":"1994","journal-title":"Semin Dermatol"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamadermatol.2019.0052"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404699"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-03-007013"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1080\/10428194.2017.1339879"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2019.11.001"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI129126"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2020-134942"},{"key":"e_1_2_7_35_1","unstructured":"Syndax Announces Positive Interim Data Demonstrating Robust Clinical Activity in Phase 1 Portion of the AUGMENT\u2010101 Trial of SNDX\u20105613 in Patients with Genetically\u2010Defined Acute Leukemias. Accessed June 7 2021.https:\/\/ir.syndax.com\/news\u2010releases\/news\u2010release\u2010details\/syndax\u2010announces\u2010positive\u2010interim\u2010data\u2010demonstrating\u2010robust"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsmedchemlett.8b00342"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-018-0056-6"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2020.11.005"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0903840"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijrobp.2018.05.045"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1716984"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2017-04-779405"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1902688"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12185-020-02855-4"}],"container-title":["Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cncr.34459","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cncr.34459","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/acsjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cncr.34459","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T13:02:59Z","timestamp":1760274179000},"score":1,"resource":{"primary":{"URL":"https:\/\/acsjournals.onlinelibrary.wiley.com\/doi\/10.1002\/cncr.34459"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,15]]},"references-count":43,"journal-issue":{"issue":"21","published-print":{"date-parts":[[2022,11]]}},"alternative-id":["10.1002\/cncr.34459"],"URL":"https:\/\/doi.org\/10.1002\/cncr.34459","archive":["Portico"],"relation":{},"ISSN":["0008-543X","1097-0142"],"issn-type":[{"value":"0008-543X","type":"print"},{"value":"1097-0142","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,15]]},"assertion":[{"value":"2022-05-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-09-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}