{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T11:51:43Z","timestamp":1792929103363,"version":"4.5.0"},"reference-count":29,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2023,3,18]],"date-time":"2023-03-18T00:00:00Z","timestamp":1679097600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP18K16292"],"award-info":[{"award-number":["JP18K16292"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["19K07707"],"award-info":[{"award-number":["19K07707"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["20H03668"],"award-info":[{"award-number":["20H03668"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["21K15963"],"award-info":[{"award-number":["21K15963"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Obtaining sufficient pancreaticobiliary tumor tissue for genomic profiling has limitations. Liquid biopsies using plasma do not provide sufficient sensitivity. Thus, this study aimed to determine the effectiveness of liquid biopsy between bile and plasma for identifying oncogenic and drug\u2010matched mutations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This study created a panel of 60 significantly mutated genes specific to pancreaticobiliary cancer (PBCA) and used it for genomic analysis of 212 deoxyribonucleic acid (DNA) samples (87 bile supernatant, 87 bile precipitate, and 38 plasma) from 87 patients with PBCA. The quantity of extracted DNA from bile and plasma was compared, as were genomic profiles of 38 pairs of bile and plasma from 38 patients with PBCA. Finally, we investigated 87 bile and 38 plasma for the ability to detect druggable mutations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The amount of DNA was significantly lower in plasma than in bile (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a0.001). Oncogenic mutations were identified in 21 of 38 (55%) patients in bile and nine (24%) in plasma samples (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a0.005). Bile was significantly more sensitive than plasma in identifying druggable mutations (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a0.032). The authors detected 23 drug\u2010matched mutations in combined bile and plasma, including five\n                      <jats:italic>ERBB2<\/jats:italic>\n                      , four\n                      <jats:italic>ATM<\/jats:italic>\n                      , three\n                      <jats:italic>BRAF<\/jats:italic>\n                      , three\n                      <jats:italic>BRCA2<\/jats:italic>\n                      , three\n                      <jats:italic>NF1<\/jats:italic>\n                      , two\n                      <jats:italic>PIK3CA<\/jats:italic>\n                      , one\n                      <jats:italic>BRCA1<\/jats:italic>\n                      , one\n                      <jats:italic>IDH1<\/jats:italic>\n                      , and one\n                      <jats:italic>PALB2<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Liquid biopsy using bile may be useful in searching for therapeutic agents, and using the obtained genomic information may improve the prognoses of patients with PBCA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Plain Language Summary<\/jats:title>\n                    <jats:p>\n                      <jats:list list-type=\"bullet\">\n                        <jats:list-item>\n                          <jats:p>Genomic profiling of formalin\u2010fixed paraffin\u2010embedded tissues may provide actionable targets for molecular and immuno\u2010oncological treatment.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>However, most pancreaticobiliary malignancies are unresectable and formalin\u2010fixed paraffin\u2010embedded tissues cannot be obtained.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Although comprehensive genomic profiling tests using plasma have been used in recent years, the utility of those using bile is not clear.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Our study revealed that bile identified more drug\u2010matched mutations than plasma in advanced pancreaticobiliary cancer patients.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Bile may help widen the patient population benefiting from targeted drugs.<\/jats:p>\n                        <\/jats:list-item>\n                      <\/jats:list>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cncr.34717","type":"journal-article","created":{"date-parts":[[2023,3,18]],"date-time":"2023-03-18T10:43:21Z","timestamp":1679136201000},"page":"1714-1722","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Comparison of genomic profiling of circulating tumor DNA in\u00a0pancreaticobiliary malignancies in plasma and bile"],"prefix":"10.1002","volume":"129","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0614-581X","authenticated-orcid":false,"given":"Hiroshi","family":"Ohyama","sequence":"first","affiliation":[{"name":"Department of Gastroenterology Graduate School of Medicine Chiba University  Chiba Japan"},{"name":"Genome Analysis Center Yamanashi Central Hospital  Yamanashi Japan"},{"name":"Department of Gastroenterology Yamanashi Central Hospital  Yamanashi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yosuke","family":"Hirotsu","sequence":"additional","affiliation":[{"name":"Genome Analysis Center Yamanashi Central Hospital  Yamanashi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenji","family":"Amemiya","sequence":"additional","affiliation":[{"name":"Genome Analysis Center Yamanashi Central Hospital  Yamanashi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshifumi","family":"Miura","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Graduate School of Medicine Chiba University  Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumio","family":"Hirose","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Yamanashi Central Hospital  Yamanashi 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