{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T09:50:04Z","timestamp":1789206604720,"version":"build-2803163510"},"reference-count":15,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T00:00:00Z","timestamp":1760486400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005010","name":"Associazione Italiana per la Ricerca sul Cancro","doi-asserted-by":"publisher","award":["20133"],"award-info":[{"award-number":["20133"]}],"id":[{"id":"10.13039\/501100005010","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer"],"published-print":{"date-parts":[[2025,10,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The authors previously demonstrated that in peripheral blood (PB) of chronic myeloid leukemia (CML), patients\u2019 leukemia stem cells (LSCs) CD26+ are detectable by flow cytometry at diagnosis, during tyrosine kinase inhibitor (TKI) therapy, and during treatment\u2010free remission.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This study presents results of a prospective multicenter study including 242 newly diagnosed CML patients monitored for PB CD26+ leukemic stem cells (LSCs) quantification from diagnosis up to 24 months of TKI treatment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The bulk of CD26+ LSCs at diagnosis varied between patients with a median value of 7.14 cells\/\u00b5L. During TKI treatment, it has been observed their consistent and rapid reduction without statistical differences according to type of first\u2010line TKI. Instead, a significant correlation between a low amount of CD26+ LSCs at diagnosis and an optimal molecular response at 3, 12, and 24 months was documented (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a0.03,\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a0.004, and\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a0.009, respectively). Three tertiles of CD26+ LSCs correlating to molecular response were identified: &lt;3.21 cells\/\u00b5L; between 3.21 and 19.21 cells\/\u00b5L; and &gt;19.21 cells\/\u00b5L. The incidence of patients with optimal response was higher in the first CD26+ LSCs tertile respect to the third one (\n                      <jats:italic>p<\/jats:italic>\n                      = .027,\n                      <jats:italic>p<\/jats:italic>\n                      = .015, and\n                      <jats:italic>p<\/jats:italic>\n                      = .079, respectively) at all time points (3, 12 and 24 months).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This study demonstrated a correlation between the amount of CD26+ LSCs at diagnosis and the molecular response, suggesting that the number of CD26+ LSCs at diagnosis could represent an additional tool for predicting TKI response.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cncr.70122","type":"journal-article","created":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T19:43:25Z","timestamp":1760557405000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Unravelling a clinical role of peripheral blood leukemia stem cells at diagnosis in chronic myeloid leukemia patients: Final results of prospective FLOWERS study"],"prefix":"10.1002","volume":"131","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5840-2956","authenticated-orcid":false,"given":"Anna","family":"Sicuranza","sequence":"first","affiliation":[{"name":"Hematology Unit University of Siena Azienda Ospedaliera Universitaria Senese  Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Pacelli","sequence":"additional","affiliation":[{"name":"Hematology Unit University of Siena Azienda Ospedaliera Universitaria Senese  Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adele","family":"Santoni","sequence":"additional","affiliation":[{"name":"Hematology Unit University of Siena Azienda Ospedaliera Universitaria Senese  Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5228-6491","authenticated-orcid":false,"given":"Elisabetta","family":"Abruzzese","sequence":"additional","affiliation":[{"name":"Sant'Eugenio Hospital Tor Vergata University  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7571-023X","authenticated-orcid":false,"given":"Daniele","family":"Cattaneo","sequence":"additional","affiliation":[{"name":"Hematology Division Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4401-0812","authenticated-orcid":false,"given":"Alessandra","family":"Iurlo","sequence":"additional","affiliation":[{"name":"Hematology Division Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luigiana","family":"Luciano","sequence":"additional","affiliation":[{"name":"Hematology\u2010Department of Clinical Medicine and Surgery Federico II University  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Galimberti","sequence":"additional","affiliation":[{"name":"Section of Hematology Department of Clinical and Experimental Medicine University of Pisa  Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-4238-1300","authenticated-orcid":false,"given":"Valentina","family":"Giai","sequence":"additional","affiliation":[{"name":"Division of Hematology Citt\u00e0 Della Salute e Della Scienza  Turin Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Mulas","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences and Public Health University of Cagliari  Cagliari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Caocci","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences and Public Health University of Cagliari  Cagliari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federica","family":"Sor\u00e0","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze di Laboratorio ed Ematologiche Fondazione Policlinico Universitario \"A. 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