{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T05:20:09Z","timestamp":1778822409517,"version":"3.51.4"},"reference-count":20,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T00:00:00Z","timestamp":1761436800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer"],"published-print":{"date-parts":[[2025,11]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n \n In acute myeloid leukemia (AML), measurable residual disease (MRD) assessment is essential for predicting relapse and guiding therapy decision\u2010making. Nucleophosmin 1 (\n NPM1<\/jats:italic>\n ) mutations are reliable MRD markers but apply to only \u223c30% of patients with AML. Wilms tumor 1 (\n WT1<\/jats:italic>\n ) expression monitoring is applicable to a broader population but the European LeukemiaNet (ELN) threshold of 50\n WT1<\/jats:italic>\n copies per 10\n 4<\/jats:sup>\n ABL<\/jats:italic>\n copies (0.5%) may be too high, which limits sensitivity.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n With\n WT1<\/jats:italic>\n expression data from 100 healthy controls, this study established a revised\n WT1<\/jats:italic>\n threshold of seven copies per 10\n 4<\/jats:sup>\n ABL<\/jats:italic>\n copies (0.07%). Its performance was retrospectively validated against\n NPM1<\/jats:italic>\n in 308 paired follow\u2010up samples from 63 patients with\n NPM1<\/jats:italic>\n \u2010mutated AML, and against the core binding factor (CBF) \u03b2\u2010MYH11 fusion transcripts in 83 samples from 12 patients. Statistical analyses included concordance, sensitivity\/specificity, survival estimates, and model comparison with the Akaike information criterion.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Compared to the ELN cutoff, the revised threshold showed higher concordance with\n NPM1<\/jats:italic>\n (78.6% vs. 73%) and sensitivity (54% vs. 24%;\n p<\/jats:italic>\n \u00a0<\u00a0.0001) and acceptable specificity (91% vs. 98%;\n p<\/jats:italic>\n \u00a0=\u00a0.002). In survival analyses, the seven\u2010copy cutoff demonstrated stronger prognostic value than the ELN threshold, particularly after consolidation therapy. In the\n CBFB::MYH11<\/jats:italic>\n cohort, the two thresholds showed similar concordance but\n WT1<\/jats:italic>\n positivity with the revised cutoff preceded relapse in selected patients.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n In AML, the revised\n WT1<\/jats:italic>\n threshold of seven copies per 10\n 4<\/jats:sup>\n ABL<\/jats:italic>\n copies enhanced MRD sensitivity while maintaining specificity, improving concordance with\n NPM1<\/jats:italic>\n , and showing prognostic relevance. These findings support the clinical value of locally optimized\n WT1<\/jats:italic>\n thresholds, and highlight the need for prospective multicenter validation and harmonization of\n WT1<\/jats:italic>\n \u2010based MRD monitoring.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/cncr.70140","type":"journal-article","created":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T09:00:12Z","timestamp":1761469212000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Optimizing Wilms tumor 1 thresholds for measurable residual disease monitoring in acute myeloid leukemia: Improved sensitivity and concordance with nucleophosmin 1 in a single\u2010center validation study"],"prefix":"10.1002","volume":"131","author":[{"given":"Sabrina","family":"Barriere","sequence":"first","affiliation":[{"name":"Service de Th\u00e9rapie Cellulaire et H\u00e9matologie Clinique Adulte CHU Clermont\u2010Ferrand Clermont\u2010Ferrand 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