{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T13:21:56Z","timestamp":1788960116078,"version":"build-2803163510"},"reference-count":36,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T00:00:00Z","timestamp":1762387200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer"],"published-print":{"date-parts":[[2025,11,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The relapse after chimeric antigen receptor (CAR) T\u2010cell therapy remains a critical challenge, and the optimal timing and treatment strategies for CAR T urgently need to be explored. Autologous hematopoietic stem cell transplantation (auto\u2010HSCT) demonstrates comparable leukemia\u2010free survival (LFS) and overall survival (OS) in patients who rapidly achieve MRD\u2010negative complete remission (CR) compared with allogeneic HSCT (allo\u2010HSCT). Thus, combining CAR T cells with auto\u2010HSCT may represent a promising treatment strategy.<\/jats:p>\n                    <jats:p>\n                      The trial registration is\n                      <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/ClinicalTrials.gov\">ClinicalTrials.gov<\/jats:ext-link>\n                      identifier NCT05470777.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This phase 2 trial evaluated the safety and efficacy of sequential CD22\/CD19 CAR T cells combined with an auto\u2010HSCT \u201csandwich\u201d strategy in patients with Philadelphia chromosome\u2013negative (Ph\u2010negative) B\u2010cell acute lymphoblastic leukemia (B\u2010ALL), including adolescents and young adults (AYA) as well as adults who were unable or declined to allo\u2010HSCT. The primary and secondary end points were OS and LFS, respectively.<\/jats:p>\n                    <jats:p>\n                      The trial registration is\n                      <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/ClinicalTrials.gov\">ClinicalTrials.gov<\/jats:ext-link>\n                      identifier NCT05470777.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>At a median follow\u2010up of 28 months, the median OS and LFS were not reached. The 2\u2010year OS and LFS rates were 97% (95% confidence interval [CI], 90%\u2013100%) and 72% (95% CI, 58%\u201390%), respectively. All 35 patients who completed the sandwich strategy survived. Continuous MRD\u2010negative CR rates after the second CAR T\u2010cell infusion were 80% by multiparameter flow cytometry and 70% by next\u2010generation sequencing of immunoglobulin H rearrangements. OS and LFS did not differ between poor and standard genetic risk groups. Compared with the allo\u2010HSCT external control group, the sandwich strategy showed improved OS and comparable LFS. No cases of immune effector cell\u2013associated neurotoxicity syndrome or severe cytokine release syndrome were observed.<\/jats:p>\n                    <jats:p>\n                      The trial registration is\n                      <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/ClinicalTrials.gov\">ClinicalTrials.gov<\/jats:ext-link>\n                      identifier NCT05470777.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The CD22\/CD19 CAR T\u2010cell and auto\u2010HSCT sandwich strategy represents a promising approach for the treatment of Ph\u2010negative B\u2010ALL in AYA and adult patients, offering high efficacy and a favorable safety profile.<\/jats:p>\n                    <jats:p>\n                      The trial registration is\n                      <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/ClinicalTrials.gov\">ClinicalTrials.gov<\/jats:ext-link>\n                      identifier NCT05470777.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cncr.70168","type":"journal-article","created":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T06:45:14Z","timestamp":1762497914000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A phase 2 trial of a \u201csandwich\u201d strategy: Sequential CD22\/CD19 chimeric antigen receptor T\u2010cells therapy combined with autologous hematopoietic stem cell transplantation in patients with Philadelphia chromosome\u2013negative B\u2010cell acute lymphoblastic leukemia"],"prefix":"10.1002","volume":"131","author":[{"given":"Chong\u2010Sheng","family":"Qian","sequence":"first","affiliation":[{"name":"National Clinical Research Center for Hematologic Diseases Jiangsu 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