{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T13:42:18Z","timestamp":1770903738500,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2010,5,13]],"date-time":"2010-05-13T00:00:00Z","timestamp":1273708800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2010,6,25]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND:<\/jats:title><jats:p>MIB\u20101 proliferation index (PI) has proven helpful for diagnosis and prognosis in non\u2010Hodgkin lymphomas (NHLs). However, validated cutoff values for use in fine\u2010needle aspiration (FNA) samples are not available. We investigated MIB\u20101 immunocytochemistry as an ancillary technique for stratifying NHL and attempted to establish PI cutpoints in cytologic samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS:<\/jats:title><jats:p>B\u2010cell NHL FNA cases with available cytospins (CS) MIB\u20101 immunocytochemistry results were included. Demographic, molecular, immunophenotyping and MIB\u20101 PI data were collected from cytologic reports. Cases were subtyped according to the current World Health Organization classification and separated into indolent, aggressive, and highly aggressive groups. Statistical analysis was performed with pairwise Wilcoxon rank sum test and linear discriminant analysis to suggest appropriate PI cutpoints.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS:<\/jats:title><jats:p>Ninety\u2010one NHL cases were subdivided in 56 (61.5%) indolent, 30 (33%) aggressive, and 5 (5.5%) highly aggressive lymphomas. The 3 groups had significantly different MIB\u20101 PIs from each other. Cutpoints were established for separating indolent (&lt;38%), aggressive (\u226538% to \u226480.1%) and highly aggressive (&gt;80.1%). The groups were adequately predicted in 76 cases (83.5%) using the cutpoints and 15 cases showed discrepant PIs.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS:<\/jats:title><jats:p>MIB\u20101 immunohistochemistry on CS can help to stratify B\u2010cell NHL and showed a significant increase in PI with tumor aggressiveness. Six misclassified cases had PIs close to the cutpoints. Discrepant MIB\u20101 PIs were related to dilution of positive cells by non\u2010neoplastic lymphocytes and to the overlapping continuum of features between diffuse large B\u2010cell lymphoma and Burkitt lymphoma. Validation of our approach in an unrelated, prospective dataset is required. Cancer (Cancer Cytopathol) 2010. \u00a9 2010 American Cancer Society.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncy.20075","type":"journal-article","created":{"date-parts":[[2010,5,14]],"date-time":"2010-05-14T10:43:42Z","timestamp":1273833822000},"page":"166-172","source":"Crossref","is-referenced-by-count":25,"title":["Classifying B\u2010cell non\u2010Hodgkin lymphoma by using MIB\u20101 proliferative index in fine\u2010needle aspirates"],"prefix":"10.1002","volume":"118","author":[{"given":"Abdullah E.","family":"Ali","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric K.","family":"Morgen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William R.","family":"Geddie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott L.","family":"Boerner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine","family":"Massey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denis J.","family":"Bailey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gilda","family":"da Cunha Santos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,6,11]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.43.4.325"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0347(199808)20:5<392::AID-HED6>3.0.CO;2-T"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/dc.2840120308"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.pas.0000172191.87176.3b"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1309\/16UL-W4PX-HRL7-7V88"},{"key":"e_1_2_6_7_2","first-page":"46","article-title":"Diagnosis and grading of non\u2010Hodgkin's lymphomas on fine needle aspiration cytology","volume":"50","author":"Mathur S","year":"2007","journal-title":"Indian J Pathol Microbiol"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1385\/MO:23:1:83"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.22173"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.21350"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1159\/000326303"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2303.1999.00065.x"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2303.2000.00234.x"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(19990825)87:4<216::AID-CNCR9>3.0.CO;2-2"},{"key":"e_1_2_6_15_2","first-page":"179","article-title":"Proliferation markers in non\u2010Hodgkin's lymphoma. 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