{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T10:06:23Z","timestamp":1771063583252,"version":"3.50.1"},"reference-count":23,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2010,8,31]],"date-time":"2010-08-31T00:00:00Z","timestamp":1283212800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2010,10,25]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND:<\/jats:title><jats:p>Excessive mucus, as well as blood and inflammation, can be problematic in the processing and screening of liquid\u2010based cervical Pap preparations by interfering in the process of cell retrieval onto specimen filters or slides. This study compares the capacity of the BD SurePath and ThinPrep liquid\u2010based Papanicolaou (Pap) tests to handle mucus\u2010laden specimens.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS:<\/jats:title><jats:p>A 100 \u03bcL volume of pooled cervical epithelial cells was added to BD SurePath and ThinPrep liquid Pap test vials. Aliquots of cervical mucus that had been collected and pooled from previously processed mucus\u2010rich Pap specimens were added to each series of test vials in amounts of 250 \u03bcL, 500 \u03bcL, 1000 \u03bcL, and 2000 \u03bcL. The vials were then routinely processed on their respective instruments and the test slides evaluated for cellularity of the squamous cell component.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS:<\/jats:title><jats:p>The BD SurePath test specimens showed no reduction of the squamous cell component resulting from the addition of any of the aliquots of mucus. The ThinPrep test specimens showed a marked loss of cellularity upon the addition of the first aliquot of mucus, with large areas of the filters showing nearly complete absence of squamous cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS:<\/jats:title><jats:p>Excessive mucus remains a potentially limiting factor in the ThinPrep Pap system. This appears to be the result of direct obstruction of the filtration membrane causing a markedly diminished retrieval of squamous epithelial cells and potentially impacting disease detection. The BD SurePath system, conversely, manages mucus by a cell enrichment process, which results in undiminished cell recovery as observed in this study's test conditions. Cancer (Cancer Cytopathol) 2010. \u00a9 2010 American Cancer Society.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncy.20096","type":"journal-article","created":{"date-parts":[[2010,8,31]],"date-time":"2010-08-31T19:23:34Z","timestamp":1283282614000},"page":"244-249","source":"Crossref","is-referenced-by-count":40,"title":["Comparison of BD Surepath and ThinPrep Pap systems in the processing of mucus\u2010rich specimens"],"prefix":"10.1002","volume":"118","author":[{"given":"Shelly","family":"Kenyon","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brenda J.","family":"Sweeney","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James","family":"Happel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gloria E.","family":"Marchilli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Weinstein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas","family":"Schneider","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,8,31]]},"reference":[{"key":"e_1_2_6_2_2","volume-title":"The Bethesda System for Reporting Cervical Cytology","author":"Birdsong G","year":"2004"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/00128360-199704000-00024"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1309\/XMQP-9AJD-104W-07C8"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.11102"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.11932"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1159\/000326863"},{"key":"e_1_2_6_8_2","first-page":"575","article-title":"Technical aspect of ThinPrep","volume":"41","author":"Song LH","year":"2000","journal-title":"Singapore Med J."},{"key":"e_1_2_6_9_2","volume-title":"(Product Insert). Volume 85193\u2013003 Rev J","author":"ThinPrep","year":"2001"},{"key":"e_1_2_6_10_2","unstructured":"TriPath Imaging I. PrepStain System Product Insert. 2004; Doc No. 61CR000021 Rev 4."},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/dc.20607"},{"key":"e_1_2_6_12_2","first-page":"958","article-title":"Comparison of cytologic preparations in cervicovaginal cytopathology: Conventional smear vs. ThinPrep vs. AutoCyte PREP","volume":"46","author":"McGrath CM","year":"2002","journal-title":"Acta Cytol."},{"key":"e_1_2_6_13_2","first-page":"958","article-title":"Impact of converting ThinPrep to SurePath on overall sensitivity and adequacy","volume":"46","author":"Fite SK","year":"2002","journal-title":"Acta Cytol."},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.21425"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1159\/000332302"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0029-7844(97)00228-7"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1159\/000331548"},{"key":"e_1_2_6_18_2","first-page":"500","article-title":"Comparison of fluid\u2010based, thin\u2010layer processing and conventional Papanicolaou methods for uterine cervical cytology","volume":"98","author":"Wang TY","year":"1999","journal-title":"J Formos Med Assoc."},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/dc.10033"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1159\/000325921"},{"key":"e_1_2_6_21_2","first-page":"1246","article-title":"Reprocessing of unsatisfactory ThinPrep samples: Procedures to improve specimen adequacy and increase detection of cervical abnormalities","volume":"42","author":"Papillo JL","year":"1998","journal-title":"Acta Cytol."},{"key":"e_1_2_6_22_2","unstructured":"BD Surepath Cell Enrichment Process for Liquid\u2010based Pap Test. BD website:http:\/\/www.bd.com\/tripath\/labs\/sp_cell_enrich.asp. Accessed June 8 2010."},{"key":"e_1_2_6_23_2","unstructured":"The ThinPrep Pap Test: The ThinPrep 2000. ThinPrep website:http:\/\/www.thinprep.com\/hcp\/lab_professionals\/imaging_ system\/thinprep_2000.html. Accessed June 8 2010."},{"key":"e_1_2_6_24_2","first-page":"681","article-title":"Comparison of methods for reprocessing bloody gynecological specimens in PreservCyt solution","volume":"48","author":"Hecht S","year":"2004","journal-title":"Acta Cytol."}],"container-title":["Cancer Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcncy.20096","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/acsjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cncy.20096","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T20:00:34Z","timestamp":1760299234000},"score":1,"resource":{"primary":{"URL":"https:\/\/acsjournals.onlinelibrary.wiley.com\/doi\/10.1002\/cncy.20096"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,8,31]]},"references-count":23,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2010,10,25]]}},"alternative-id":["10.1002\/cncy.20096"],"URL":"https:\/\/doi.org\/10.1002\/cncy.20096","archive":["Portico"],"relation":{},"ISSN":["1934-662X","1934-6638"],"issn-type":[{"value":"1934-662X","type":"print"},{"value":"1934-6638","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,8,31]]}}}