{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:25:05Z","timestamp":1770823505726,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2011,7,5]],"date-time":"2011-07-05T00:00:00Z","timestamp":1309824000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2011,12,25]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND:<\/jats:title><jats:p>Fluorescence in situ hybridization (FISH) is routinely used to help clarify atypical urinary cytology. However, to the authors' knowledge, little is known regarding the frequency of chromosomal aberrations in non\u2010neoplastic conditions that could potentially lead to false\u2010positive FISH results. The objective of the current study was to evaluate the frequency of chromosomal aberrations in benign cells of the urinary tract using the UroVysion FISH test.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS:<\/jats:title><jats:p>The authors analyzed 77 Papanicolaou\u2010stained benign urine cytology specimens with reactive epithelial atypia using a FISH assay detecting the chromosomes 3, 7, and 17 and the gene locus 9p21. A positive test result was defined as an increased copy number of at least 2 chromosomes in \u2265 4 of 25 cells, or &gt; 10 cells with a tetraploid or octaploid pattern, or homozygous or heterozygous deletion of 9p21 (\u2265 12cells).<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS:<\/jats:title><jats:p>FISH was positive in 27 of 77 bladder washings (35.1%) including 25 of 65 bladder washings (40.5%) and 2 of 15 voided urines (13.5%) from patients with irritative bladder (15 of 36 patients), a history of radiotherapy (7 of 12 patients), nonspecific cystitis (3 of 11 patients), hematuria (3 of 8 patients), and lithiasis (1 of 4 patients) . In 7 of 27 FISH\u2010positive urothelial specimens, the positivity was solely due a polyploid pattern (tetraploid\/octaploid pattern) in &gt; 10 of the cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS:<\/jats:title><jats:p>Chromosomal aberrations can occur in reactive urothelial cells, with a tetraploid pattern being the most common. Even an aneuploid pattern of FISH signals does not always prove malignancy because it rarely occurs in reactive urothelial cells. Correlation of FISH results with cytomorphology and patient history is crucial to avoid false\u2010positive diagnoses. Cancer (Cancer Cytopathol) 2011;. \u00a9 2011 American Cancer Society.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncy.20171","type":"journal-article","created":{"date-parts":[[2011,7,5]],"date-time":"2011-07-05T12:28:35Z","timestamp":1309868915000},"page":"404-410","source":"Crossref","is-referenced-by-count":33,"title":["Evaluation of chromosomal aberrations in patients with benign conditions and reactive changes in urinary cytology"],"prefix":"10.1002","volume":"119","author":[{"given":"Coya","family":"Tapia","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathrina","family":"Glatz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ellen C.","family":"Obermann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno","family":"Grilli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Audrey","family":"Barascud","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle","family":"Herzog","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ren\u00e9","family":"Sch\u00f6negg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Spasenija","family":"Savic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lukas","family":"Bubendorf","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,7,5]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02552160"},{"key":"e_1_2_7_3_2","first-page":"539","article-title":"Chromosome abnormalities as primary events in human malignant disease: evidence from marker chromosomes","volume":"36","author":"Atkin NB","year":"1966","journal-title":"J Natl Cancer Inst."},{"key":"e_1_2_7_4_2","first-page":"309","article-title":"Interphase cytogenetic diagnosis of bladder cancer on cells from urine and bladder washing","volume":"14","author":"Junker K","year":"1999","journal-title":"Int J Oncol."},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2007.04.015"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1525-1578(10)60625-3"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/PAP.0b013e3181832320"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2010.04.026"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2009.08.157"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1309\/K5P2-4Y8B-7L5A-FAA9"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.24258"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.21704"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.10881"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2007.11.082"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2007.12.006"},{"issue":"6","key":"e_1_2_7_16_2","doi-asserted-by":"crossref","first-page":"2262","DOI":"10.1016\/S0022-5347(17)31654-3","article-title":"Abnormal flow cytometry profiles in patients with interstitial cystitis","volume":"152","author":"Bushman W","year":"1994","journal-title":"J Urol."},{"key":"e_1_2_7_17_2","first-page":"411","article-title":"Superficial urothelial (umbrella) cells. 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