{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T04:18:21Z","timestamp":1790050701463,"version":"4.0.1"},"reference-count":47,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,4,19]],"date-time":"2012-04-19T00:00:00Z","timestamp":1334793600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2012,12,25]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND:<\/jats:title><jats:p>The human immunoglobulin heavy\u2010chain (<jats:italic>IGH<\/jats:italic>) locus at chromosome 14q32 is frequently involved in different translocations of non\u2010Hodgkin lymphoma (NHL), and the detection of any breakage involving the <jats:italic>IGH<\/jats:italic> locus should identify a B\u2010cell NHL. The split\u2010signal <jats:italic>IGH<\/jats:italic> fluorescence in situ hybridization\u2010chromogenic in situ hybridization (FISH\u2010CISH) DNA probe is a mixture of 2 fluorochrome\u2010labeled DNAs: a green one that binds the telomeric segment and a red one that binds the centromeric segment, both on the <jats:italic>IGH<\/jats:italic> breakpoint. In the current study, the authors tested the capability of the <jats:italic>IGH<\/jats:italic> FISH\u2010CISH DNA probe to detect <jats:italic>IGH<\/jats:italic> translocations and diagnose B\u2010cell lymphoproliferative processes on cytological samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS:<\/jats:title><jats:p>Fifty cytological specimens from cases of lymphoproliferative processes were tested using the split\u2010signal <jats:italic>IGH<\/jats:italic> FISH\u2010CISH DNA probe and the results were compared with light\u2010chain assessment by flow cytometry (FC), <jats:italic>IGH<\/jats:italic> status was tested by polymerase chain reaction (PCR), and clinicohistological data.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS:<\/jats:title><jats:p>The signal score produced comparable results on FISH and CISH analysis and detected 29 positive, 15 negative, and 6 inadequate cases; there were 29 true\u2010positive cases (66%), 9 true\u2010negative cases (20%), 6 false\u2010negative cases (14%), and no false\u2010positive cases (0%). Comparing the sensitivity of the <jats:italic>IGH<\/jats:italic> FISH\u2010CISH DNA split probe with FC and PCR, the highest sensitivity was obtained by FC, followed by FISH\u2010CISH and PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS:<\/jats:title><jats:p>The split\u2010signal <jats:italic>IGH<\/jats:italic> FISH\u2010CISH DNA probe is effective in detecting any translocation involving the <jats:italic>IGH<\/jats:italic> locus. This probe can be used on different samples from different B\u2010cell lymphoproliferative processes, although it is not useful for classifying specific entities. Cancer (Cancer Cytopathol) 2012;. \u00a9 2012 American Cancer Society.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncy.21203","type":"journal-article","created":{"date-parts":[[2012,4,19]],"date-time":"2012-04-19T10:37:29Z","timestamp":1334831849000},"page":"390-400","source":"Crossref","is-referenced-by-count":25,"title":["Immunoglobulin heavy\u2010chain fluorescence in situ hybridization\u2010chromogenic in situ hybridization DNA probe split signal in the clonality assessment of lymphoproliferative processes on cytological samples"],"prefix":"10.1002","volume":"120","author":[{"given":"Pio","family":"Zeppa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura Virginia","family":"Sosa 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