{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T00:50:16Z","timestamp":1760316616109,"version":"build-2065373602"},"reference-count":41,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,1,29]],"date-time":"2013-01-29T00:00:00Z","timestamp":1359417600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Numerous genomic abnormalities in B\u2010cell non\u2010Hodgkin lymphomas (<jats:styled-content style=\"fixed-case\">NHLs<\/jats:styled-content>) have been revealed by novel high\u2010throughput technologies, including recurrent mutations in <jats:styled-content style=\"fixed-case\"><jats:italic>EZH2<\/jats:italic><\/jats:styled-content> (enhancer of zeste homolog 2) and <jats:styled-content style=\"fixed-case\"><jats:italic>CD79B<\/jats:italic><\/jats:styled-content> (B cell antigen receptor complex\u2010associated protein beta chain) genes. This study sought to determine the evolution of the mutational status of <jats:styled-content style=\"fixed-case\"><jats:italic>EZH2<\/jats:italic><\/jats:styled-content> and <jats:styled-content style=\"fixed-case\"><jats:italic>CD79B<\/jats:italic><\/jats:styled-content> over time in different samples from the same patient in a cohort of B\u2010cell <jats:styled-content style=\"fixed-case\">NHLs<\/jats:styled-content>, through use of a customized multiplex mutation assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> that was extracted from cytological material stored on <jats:styled-content style=\"fixed-case\">FTA<\/jats:styled-content> cards as well as from additional specimens, including archived frozen and formalin\u2010fixed histological specimens, archived stained smears, and cytospin preparations, were submitted to a multiplex mutation assay specifically designed for the detection of point mutations involving <jats:styled-content style=\"fixed-case\"><jats:italic>EZH2<\/jats:italic><\/jats:styled-content> and <jats:styled-content style=\"fixed-case\"><jats:italic>CD79B<\/jats:italic><\/jats:styled-content>, using MassARRAY spectrometry followed by Sanger sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>All 121 samples from 80 B\u2010cell <jats:styled-content style=\"fixed-case\">NHL<\/jats:styled-content> cases were successfully analyzed. Mutations in <jats:styled-content style=\"fixed-case\"><jats:italic>EZH2<\/jats:italic><\/jats:styled-content> (Y646) and <jats:styled-content style=\"fixed-case\"><jats:italic>CD79B<\/jats:italic><\/jats:styled-content> (Y196) were detected in 13.2% and 8% of the samples, respectively, almost exclusively in follicular lymphomas and diffuse large B\u2010cell lymphomas. In one\u2010third of the positive cases, a wild type was detected in a different sample from the same patient during follow\u2010up.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>Testing multiple minimal tissue samples using a high\u2010throughput multiplex platform exponentially increases tissue availability for molecular analysis and might facilitate future studies of tumor progression and the related molecular events. Mutational status of <jats:styled-content style=\"fixed-case\"><jats:italic>EZH2<\/jats:italic><\/jats:styled-content> and <jats:styled-content style=\"fixed-case\"><jats:italic>CD79B<\/jats:italic><\/jats:styled-content> may vary in B\u2010cell <jats:styled-content style=\"fixed-case\">NHL<\/jats:styled-content> samples over time and support the concept that individualized therapy should be based on molecular findings at the time of treatment, rather than on results obtained from previous specimens. Cancer (Cancer Cytopathol) 2013;121:377\u2013386. \u00a9 2013 American Cancer Society.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncy.21262","type":"journal-article","created":{"date-parts":[[2013,1,29]],"date-time":"2013-01-29T15:14:57Z","timestamp":1359472497000},"page":"377-386","source":"Crossref","is-referenced-by-count":23,"title":["<scp><i>EZH2<\/i><\/scp> and <scp><i>CD79B<\/i><\/scp> mutational status over time in B\u2010cell non\u2010Hodgkin lymphomas detected by high\u2010throughput sequencing using minimal samples"],"prefix":"10.1002","volume":"121","author":[{"given":"Mauro Ajaj","family":"Saieg","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology University of Toronto  Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William R.","family":"Geddie","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology University of Toronto  Ontario Canada"},{"name":"Laboratory Medicine Program University Health Network Toronto Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott L.","family":"Boerner","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology University of Toronto  Ontario Canada"},{"name":"Laboratory Medicine Program University Health Network Toronto Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denis","family":"Bailey","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology University of Toronto  Ontario Canada"},{"name":"Laboratory Medicine Program University Health Network Toronto Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Crump","sequence":"additional","affiliation":[{"name":"Division of Medical Oncology and Hematology University Health Network Toronto Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gilda","family":"da Cunha Santos","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology University of Toronto  Ontario Canada"},{"name":"Laboratory Medicine Program University Health Network Toronto Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,1,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1121343109"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10351"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09730"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.892"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08638"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.518"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2011.054080"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2008.11.007"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/35020506"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-04-280149"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2011.08.018"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-11-321208"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-10-2525"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-10-2156"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1634\/theoncologist.2011-0432"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.3990"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-03-272278"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2011.26"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-06-0659"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.2353\/jmoldx.2010.090238"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.20205"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0017791"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.20166"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.20075"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.20098"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.25393"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2010.311"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(11)70335-7"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.3109\/10428199909050950"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2559.2001.01120.x"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0028585"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-08-0752"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2010.08445.x"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2010.08.018"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-10-101"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2010.11.005"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/bcr2603"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2011.110"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0007887"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1975"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0004.2008.01071.x"}],"container-title":["Cancer Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcncy.21262","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cncy.21262","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cncy.21262","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/acsjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cncy.21262","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T17:04:14Z","timestamp":1760288654000},"score":1,"resource":{"primary":{"URL":"https:\/\/acsjournals.onlinelibrary.wiley.com\/doi\/10.1002\/cncy.21262"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,1,29]]},"references-count":41,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2013,7]]}},"alternative-id":["10.1002\/cncy.21262"],"URL":"https:\/\/doi.org\/10.1002\/cncy.21262","archive":["Portico"],"relation":{},"ISSN":["1934-662X","1934-6638"],"issn-type":[{"type":"print","value":"1934-662X"},{"type":"electronic","value":"1934-6638"}],"subject":[],"published":{"date-parts":[[2013,1,29]]}}}