{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:25:52Z","timestamp":1788827152915,"version":"build-2803163510"},"reference-count":38,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2013,2,13]],"date-time":"2013-02-13T00:00:00Z","timestamp":1360713600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Cytology is an excellent method with which to diagnose preinvasive lesions of the uterine cervix, but it suffers from limited specificity for clinically significant lesions. Supplementary methods might predict the natural course of the detected lesions. The objective of the current study was to test whether a multicolor fluorescence in situ hybridization (FISH) assay might help to stratify abnormal results of Papanicolaou tests.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>A total of 219 liquid\u2010based cytology specimens of low\u2010grade squamous intraepithelial lesions (LSIL), 49 atypical squamous cells of undetermined significance (ASCUS) specimens, 52 high\u2010grade squamous intraepithelial lesion (HSIL) specimens, and 50 normal samples were assessed by FISH with probes for the human papillomavirus (HPV), <jats:italic>MYC,<\/jats:italic> and telomerase RNA component (<jats:italic>TERC<\/jats:italic>). Subtyping of HPV by polymerase chain reaction (PCR) was performed in a subset of cases (n=206).<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>There was a significant correlation found between HPV detection by FISH and PCR (<jats:italic>P<\/jats:italic>&lt;.0001). In patients with LSILs, the presence of HPV detected by FISH was significantly associated with disease progression (<jats:italic>P<\/jats:italic>&lt;.0001). An increased <jats:italic>MYC<\/jats:italic> and\/or <jats:italic>TERC<\/jats:italic> gene copy number (&gt;2 signals in&gt;10% of cells) prevailed in 43% of ASCUS specimens and was more frequent in HSIL (85%) than in LSIL (33%) (HSIL vs LSIL: <jats:italic>P<\/jats:italic>&lt;.0001). Increased <jats:italic>TERC<\/jats:italic> gene copy number was significantly correlated with progression of LSIL (<jats:italic>P<\/jats:italic>&lt;.01; odds ratio, 7.44; area under the receiver operating characteristic curve, 0.73; positive predictive value, 0.30; negative predictive value, 0.94)<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>The detection of HPV by FISH analysis is feasible in liquid\u2010based cytology and is significantly correlated with HPV analysis by PCR. The analysis of <jats:italic>TERC<\/jats:italic> gene copy number may be useful for risk stratification in patients with LSIL. <jats:bold><jats:italic>Cancer (Cancer Cytopathol)<\/jats:italic> 2013;121:423\u201331.<\/jats:bold> \u00a9 <jats:italic>2013 American Cancer Society<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncy.21280","type":"journal-article","created":{"date-parts":[[2013,2,13]],"date-time":"2013-02-13T12:04:36Z","timestamp":1360757076000},"page":"423-431","source":"Crossref","is-referenced-by-count":7,"title":["Prediction of outcome in patients with low\u2010grade squamous intraepithelial lesions by fluorescence in situ hybridization analysis of human papillomavirus, <i>TERC<\/i>, and <i>MYC<\/i>"],"prefix":"10.1002","volume":"121","author":[{"given":"Ellen C.","family":"Obermann","sequence":"first","affiliation":[{"name":"Institute of Pathology, University Hospital Basel Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Spasenija Savic","family":"Prince","sequence":"additional","affiliation":[{"name":"Institute of Pathology, University Hospital Basel Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audrey","family":"Barascud","sequence":"additional","affiliation":[{"name":"Institute of Pathology, University Hospital Basel Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Grilli","sequence":"additional","affiliation":[{"name":"Institute of Pathology, University Hospital Basel Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Herzog","sequence":"additional","affiliation":[{"name":"Institute of Pathology, University Hospital Basel Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Kaup","sequence":"additional","affiliation":[{"name":"Institute of Pathology Liestal, Kantonsspital Baselland Liestal Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gieri","family":"Cathomas","sequence":"additional","affiliation":[{"name":"Institute of Pathology Liestal, Kantonsspital Baselland Liestal Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brigitte Frey","family":"Tirri","sequence":"additional","affiliation":[{"name":"Womens' Hospital, Kantonsspital Baselland Bruderholz Bruderholz Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inti","family":"Zlobec","sequence":"additional","affiliation":[{"name":"Institute of Pathology, University of Bern Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward","family":"Wight","sequence":"additional","affiliation":[{"name":"Womens' Hospital, University Hospital Basel Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lukas","family":"Bubendorf","sequence":"additional","affiliation":[{"name":"Institute of Pathology, University Hospital Basel Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,2,13]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1989.03420050087046"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/92.5.397"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0b013e31818f5008"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2008.05.002"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc798"},{"key":"e_1_2_7_7_1","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1093\/jnci\/93.4.293","article-title":"ALTS Study Group. 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