{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T13:58:20Z","timestamp":1770472700285,"version":"3.49.0"},"reference-count":68,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2013,7,23]],"date-time":"2013-07-23T00:00:00Z","timestamp":1374537600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>High\u2010risk human papillomavirus (hrHPV) DNA detection is generally accepted for the triage of women with a cytologic diagnosis of atypical squamous cells of undetermined significance (ASC\u2010US). However, no consensus has been reached on the optimal management of low\u2010grade squamous intraepithelial lesions (LSIL).<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>In this meta\u2010analysis, the diagnostic accuracy of nucleic acid sequence\u2010based amplification (NASBA) detection of messenger ribonucleic acid (mRNA) of 5 hrHPV types (the PreTect HPV\u2010Proofer and NucliSENS EasyQ tests) for detecting grade 2 cervical intraepithelial neoplasia or worse (CIN2+) and CIN3+ was assessed in women who had a diagnosis of ASC\u2010US and LSIL. The results were compared with the Hybrid Capture\u20102 (HC2) assay, which detects the DNA of 13 hrHPV types. A bivariate random\u2010effect model that incorporated the intrinsic correlation between the true\u2010positive and false\u2010positive rates was used for a pooled meta\u2010analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Considering underlying CIN2+, the pooled absolute sensitivity of the 10 included studies was 75.4% (95% confidence interval [CI], 68.1%\u201082.7%) and 76.2% (95% CI, 68.3%\u201076.9%) for the triage of ASC\u2010US and LSIL, respectively. The pooled absolute specificity to exclude CIN2+ was 77.9% (95% CI, 70.1%\u201085.7%) and 74.2% (95% CI, 69.5%\u201078.8%) in women with ASC\u2010US and LSIL, respectively. Five studies allowed direct comparison of the mRNA assays with HC2. Considering CIN2+ in women with ASC\u2010US and LSIL, mRNA testing was substantially more specific than the HC2 assay (ratio: 1.98 and 3.36, respectively; <jats:italic>P<\/jats:italic>\u2009&lt;\u2009.001) but was less sensitive (ratio: 0.80 and 0.74, respectively; <jats:italic>P<\/jats:italic>\u2009&lt;\u2009.001).<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>HPV assays for detecting the mRNA of 5 hrHPV types may reduce the over\u2010diagnosis of women who have minor cytologic abnormalities. However, given the lower sensitivity, women with negative mRNA test results cannot be considered free of CIN2+ and require further surveillance. <jats:bold><jats:italic>Cancer (Cancer Cytopathol)<\/jats:italic> 2013;121:675\u2013687<\/jats:bold>. \u00a9 <jats:italic>2013 American Cancer Society<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncy.21325","type":"journal-article","created":{"date-parts":[[2013,7,24]],"date-time":"2013-07-24T09:40:07Z","timestamp":1374658807000},"page":"675-687","source":"Crossref","is-referenced-by-count":36,"title":["Triage of women with minor abnormal cervical cytology: Meta\u2010analysis of the accuracy of an assay targeting messenger ribonucleic acid of 5 high\u2010risk human papillomavirus types"],"prefix":"10.1002","volume":"121","author":[{"given":"Freija","family":"Verdoodt","sequence":"first","affiliation":[{"name":"Unit of Cancer Epidemiology Scientific Institute of Public Health Brussels 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