{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:50:30Z","timestamp":1771462230636,"version":"3.50.1"},"reference-count":16,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,12,3]],"date-time":"2013-12-03T00:00:00Z","timestamp":1386028800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>The authors previously demonstrated that lysing samples with glacial acetic acid (GAA) before human papillomavirus (HPV) testing does not adversely affect HPV detection with the cobas 4800 HPV Test. However, the long\u2010term impact of GAA on HPV DNA was not explored in that study, and inherent cell loss with the lysing protocol used also was observed. The current study considered the long\u2010term effects of GAA treatment of cervical ThinPrep samples on HPV detection with the cobas 4800 HPV Test. They also modified the manufacturer's lysing procedure for ThinPrep specimens to help prevent cell loss.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>Seventy\u2010eight ThinPrep samples, including previously lysed, archived specimens, were split; then, 1 part was treated with GAA according to the manufacturer's protocol or using a modified protocol, and the other part was left untreated. All samples were tested for HPV using the cobas 4800 HPV Test. The HPV\u2010positive\/HPV\u2010negative status of tested samples was used to determine the level of agreement between treated and untreated fractions. Performance of the modified lysing procedure was assessed relative to the manufacturer's protocol.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Positive HPV status produced 97% agreement between the GAA\u2010treated\/untreated fractions. Concordance between the GAA\u2010treated\/untreated fractions did not differ between the 2 lysing methods; however, the average percentage increases in \u03b2\u2010globin and HPV cycle threshold values after lysing were less discernible for the modified lysing method.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>GAA treatment of cervical ThinPrep specimens does not have long\u2010term adverse effects on HPV detection with the cobas 4800 HPV Test. The manufacturer's GAA lysing procedure for ThinPrep specimens can be reliably modified for specimens that may subsequently undergo HPV testing. <jats:bold><jats:italic>Cancer (Cancer Cytopathol)<\/jats:italic> 2014;122:250\u2013256<\/jats:bold>. \u00a9 <jats:italic>2013 American Cancer Society<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncy.21377","type":"journal-article","created":{"date-parts":[[2013,12,3]],"date-time":"2013-12-03T15:38:02Z","timestamp":1386085082000},"page":"250-256","source":"Crossref","is-referenced-by-count":10,"title":["Stability of human papillomavirus (HPV) in cervical ThinPrep specimens previously lysed with glacial acetic acid: Effect on cobas 4800 HPV test performance"],"prefix":"10.1002","volume":"122","author":[{"given":"Mary","family":"McMenamin","sequence":"first","affiliation":[{"name":"Cytopathology Department Altnagelvin Hospital Western Health and Social Care Trust Londonderry Northern Ireland United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"McKenna","sequence":"additional","affiliation":[{"name":"Cytopathology Department Altnagelvin Hospital Western Health and Social Care Trust Londonderry Northern Ireland United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,12,3]]},"reference":[{"key":"e_1_2_7_2_1","volume-title":"Evaluation of HPV\/LBC Cervical Screening Pilot Studies: Report to the Department of Health (Revised October 2004)","author":"Moss SM","year":"2004"},{"key":"e_1_2_7_3_1","volume-title":"Annual Report and Statistical Bulletin, 2009\u20102010","author":"Northern Ireland Cervical Screening Programme","year":"2010"},{"key":"e_1_2_7_4_1","volume-title":"NHS Cancer Screening Programmes (CSP) Statistical Bulletin, England 2010\u20102011","author":"The National Health Service (NHS) Information Centre, Screening and Immunisation Team","year":"2011"},{"key":"e_1_2_7_5_1","volume-title":"A Report on the Implementation of Liquid Based Cytology in Wales: July 2004 to December 2005","author":"Cervical Screening Wales","year":"2007"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa071430"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(09)70360-2"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.3310\/hta13510"},{"key":"e_1_2_7_9_1","volume-title":"Achievable Standards, Benchmarks for Reporting and Criteria for Evaluating Cervical Cytopathology. 3rd ed. NHSCSP Publ. No. 1","author":"National Health Service (NHS) Cancer Screening Programmes (CSP)","year":"2012"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.287.16.2114"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/cyt.12052"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.21349"},{"key":"e_1_2_7_13_1","volume-title":"ThinPrep 3000 Processor Operator's Manual","author":"Cytyc Corporation (Hologic)","year":"2000"},{"key":"e_1_2_7_14_1","volume-title":"cobas HPV Test Sample Color Reference Guide","author":"Roche Molecular Systems","year":"2012"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1309\/GE86-156K-EGM2-DGXB"},{"key":"e_1_2_7_16_1","volume-title":"cobas 4800 HPV Test [package insert]. 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