{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T00:49:53Z","timestamp":1760316593981,"version":"build-2065373602"},"reference-count":31,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,2,7]],"date-time":"2014-02-07T00:00:00Z","timestamp":1391731200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0\/"}],"funder":[{"name":"Italian Ministry of University and Research","award":["PRIN No 20077MBW5M7"],"award-info":[{"award-number":["PRIN No 20077MBW5M7"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Fluorescence in situ hybridization (FISH) to identify specific DNA target sequences in the nuclei of nondividing cells of numerous solid neoplasms has contributed to the introduction of molecular cytogenetics as a useful adjunct to cytology, leading recently to the \u201cmarriage\u201d of the 2 disciplines. Numerous cancer molecular markers can now be investigated using different technical approaches, at both the gene and expression levels, in biopsies of various suspected cancers, including differentiated thyroid carcinoma. The limited amount of bioptic material is often insufficient to carry out multiple tests, and optimizing handling of the biopsy is desirable.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>We have developed a home\u2010brew tetracolor break\u2010apart probe able to simultaneously identify the 2 most common genetic alterations in differentiated thyroid carcinoma: <jats:italic>RET\/PTC<\/jats:italic> variants in papillary thyroid carcinoma and <jats:italic>PAX8\/PPARg<\/jats:italic> fusion and variants in follicular thyroid carcinoma.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>The probe had 100% specificity, 99.5% sensitivity, and \u22653% cutoff. The probe was tested on <jats:italic>RET\/PTC<\/jats:italic> and <jats:italic>PAX8\/PPARg<\/jats:italic> RT\u2010PCR positive controls, and feasibility was assessed in 368 thyroid nodule fine\u2010needle aspirations (FNA). In the latter analysis, 24 FNAs had split <jats:italic>RET<\/jats:italic> signal, and 9 had split <jats:italic>PPARg<\/jats:italic> signal. FISH analysis of available surgically removed nodules confirmed the sensitivity of FISH in detecting abnormal clones and oligoclones.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>The home\u2010brew tetracolor probe showed high feasibility, optimizing the use of the biological material in relation to the available molecular tests and maximizing the FISH experimental and slide\u2010scoring times. This probe may be considered an alternative to RT\u2010PCR when recovery and quality of RNA amplification from FNA are insufficient. <jats:bold><jats:italic>Cancer (Cancer Cytopathol)<\/jats:italic> 2014;122:377\u2013385<\/jats:bold>. \u00a9 2014 The Authors. <jats:italic>Cancer Cytopathology<\/jats:italic> published by Wiley Periodicals, Inc. on behalf of American Cancer Society.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncy.21397","type":"journal-article","created":{"date-parts":[[2014,2,7]],"date-time":"2014-02-07T14:39:34Z","timestamp":1391783974000},"page":"377-385","source":"Crossref","is-referenced-by-count":5,"title":["Optimizing detection of RET and PPARg rearrangements in thyroid neoplastic cells using a home\u2010brew tetracolor probe"],"prefix":"10.1002","volume":"122","author":[{"given":"Paola","family":"Caria","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences University of Cagliari Cagliari Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela V.","family":"Frau","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences University of Cagliari Cagliari Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tinuccia","family":"Dettori","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences University of Cagliari Cagliari Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesco","family":"Boi","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences \u201cM. 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