{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T04:21:12Z","timestamp":1782879672347,"version":"3.54.5"},"reference-count":27,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2015,8,4]],"date-time":"2015-08-04T00:00:00Z","timestamp":1438646400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Precursor T\u2010lymphoblastic lymphoma (T\u2010LBL) is a rare lymphoma presenting clinically in children and adolescents with a rapidly enlarging mediastinal mass, dyspnea, and cervical lymphadenopathy requiring quick diagnosis. The objective of the current study was to report on the spectrum of cytomorphology and flow cytometric immunophenotyping (FCI).<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>The clinical profile, cytomorphological features, FCI, and cell block immunocytochemistry (CB\u2010ICC) of all cases of T\u2010LBL diagnosed from 2011 through 2013 were reviewed.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Fifteen cases of precursor T\u2010LBL (10 fine\u2010needle aspiration samples and 5 pleural\/pericardial fluid samples) were evaluated. Smears demonstrated dispersed lymphoblasts, with a high nuclear:cytoplasmic ratio and scanty basophilic cytoplasm. Nuclei demonstrated notches, clefts, and indentations. The chromatin was condensed in small and intermediate\u2010sized blasts and dispersed in larger blasts. Nucleoli were present only in the larger blasts. Hand mirror\u2010shaped cells and mitoses were variable. With regard to immunophenotyping, flow cytometry demonstrated positivity for CD2 (15 of 15 cases), surface CD3 (14 of 15 cases), cytoplasmic CD3 (15 of 15 cases), terminal deoxynucleotidyl transferase (TdT) (8 of 15 cases), CD5 (13 of 15 cases), CD10 (7 of 15 cases), and human leukocyte antigen\u2010D related (HLA\u2010DR) (1 of 15 cases). Dual CD4\/CD8 positivity was observed in all cases forming a tight cluster, which is consistent with the cortical T\u2010LBL subtype. CB\u2010ICC demonstrated a uniform CD3\u2010positive\/TdT\u2010positive\/CD20\u2010negative phenotype. In 7 cases in which TdT was negative by flow cytometry, CB\u2010ICC was positive.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>Combining cytomorphology and FCI enables the accurate and rapid diagnosis of T\u2010LBL on fine\u2010needle aspiration and effusion cytology specimens, thereby obviating the need for a biopsy. <jats:bold><jats:italic>Cancer (Cancer Cytopathol)<\/jats:italic> 2015;123:557\u2013565.<\/jats:bold> \u00a9 <jats:italic>2015 American Cancer Society.<\/jats:italic><\/jats:p><\/jats:sec>","DOI":"10.1002\/cncy.21584","type":"journal-article","created":{"date-parts":[[2015,8,4]],"date-time":"2015-08-04T10:39:21Z","timestamp":1438684761000},"page":"557-565","source":"Crossref","is-referenced-by-count":30,"title":["Precursor T\u2010lymphoblastic lymphoma: Speedy diagnosis in FNA and effusion cytology by morphology, immunochemistry, and flow cytometry"],"prefix":"10.1002","volume":"123","author":[{"given":"Poonam","family":"Bhaker","sequence":"first","affiliation":[{"name":"Department of Cytology and Gynecological Pathology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anirban","family":"Das","sequence":"additional","affiliation":[{"name":"Division of Hematology\u2010Oncology, Department of Pediatrics Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arvind","family":"Rajwanshi","sequence":"additional","affiliation":[{"name":"Department of Cytology and Gynecological Pathology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Upasana","family":"Gautam","sequence":"additional","affiliation":[{"name":"Department of Cytology and Gynecological Pathology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amita","family":"Trehan","sequence":"additional","affiliation":[{"name":"Division of Hematology\u2010Oncology, Department of Pediatrics Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deepak","family":"Bansal","sequence":"additional","affiliation":[{"name":"Division of Hematology\u2010Oncology, Department of Pediatrics Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neelam","family":"Varma","sequence":"additional","affiliation":[{"name":"Department of Hematology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radhika","family":"Srinivasan","sequence":"additional","affiliation":[{"name":"Department of Cytology and Gynecological Pathology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,8,4]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.10552"},{"key":"e_1_2_7_3_1","first-page":"176","volume-title":"WHO Classification of Tumours of Hematopoietic and Lymphoid Tissues","author":"Borowitz MJ","year":"2008"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197812282992606"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2003.10.116"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/10428190310001598008"},{"key":"e_1_2_7_7_1","first-page":"887","article-title":"Fine needle aspiration of lymphoblastic lymphoma. 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