{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T05:36:11Z","timestamp":1791005771504,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2015,8,4]],"date-time":"2015-08-04T00:00:00Z","timestamp":1438646400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Human papillomavirus (HPV)\u2010related oropharyngeal squamous cell carcinoma (SCC) is a unique form of carcinoma that is important to identify for prognosis and treatment. Immunohistochemistry (IHC) for p16 (also known as cyclin\u2010dependent kinase inhibitor 2A, multiple tumor suppressor 1) is used as a surrogate marker for transcriptionally active, high\u2010risk HPV. The primary objective of this study was to correlate p16 IHC of cell blocks from fine\u2010needle aspirations (FNAs) with surgical pathology specimens of HPV\u2010related oropharyngeal SCC.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>In total, 48 patients who had a diagnosis of oropharyngeal or nonoropharyngeal SCC and also had an FNA that demonstrated metastatic SCC with available cell block material were identified. IHC for p16 was evaluated on both FNA cell blocks and surgical pathology specimens. In situ hybridization for high\u2010risk HPV messenger RNA was performed on 31 of the FNA cell blocks.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Although partial p16 staining was observed in the majority of cell blocks, there was concordance in 47 of 48 FNAs (98%) with surgical pathology specimens when strong positive p16 staining of at least 15% of tumor cells in FNA cell block material was present. In addition, high\u2010risk HPV RNA in situ hybridization demonstrated a high correlation with p16 staining in surgical pathology specimens (96%) and FNAs (93%).<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>There was excellent correlation between p16 IHC of FNA cell blocks and surgical pathology specimens using a cutoff of at least 15% positive staining in cell blocks. The recommended threshold (70% positive staining) for surgical pathology specimens may yield a high rate of false\u2010negative results if applied to FNA cell blocks. <jats:bold><jats:italic>Cancer (Cancer Cytopathol)<\/jats:italic> 2015;123:723\u2013731.<\/jats:bold> \u00a9 <jats:italic>2015 American Cancer Society<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncy.21600","type":"journal-article","created":{"date-parts":[[2015,8,4]],"date-time":"2015-08-04T11:02:45Z","timestamp":1438686165000},"page":"723-731","source":"Crossref","is-referenced-by-count":61,"title":["Correlation of p16 immunohistochemistry in FNA biopsies with corresponding tissue specimens in HPV\u2010related squamous cell carcinomas of the oropharynx"],"prefix":"10.1002","volume":"123","author":[{"given":"Jalal B.","family":"Jalaly","sequence":"first","affiliation":[{"name":"Department of Pathology and Immunology Washington University School of Medicine St. Louis Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"James S.","family":"Lewis","sequence":"additional","affiliation":[{"name":"Department of Pathology and Immunology Washington University School of Medicine St. Louis Missouri"},{"name":"Department of Pathology Microbiology, and Immunology, Vanderbilt University Medical Center Nashville Tennessee"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian T.","family":"Collins","sequence":"additional","affiliation":[{"name":"Department of Pathology and Immunology Washington University School of Medicine St. Louis Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingyong","family":"Wu","sequence":"additional","affiliation":[{"name":"Advanced Cell Diagnostics, Inc Hayward California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010Jun","family":"Ma","sequence":"additional","affiliation":[{"name":"Advanced Cell Diagnostics, Inc Hayward California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuling","family":"Luo","sequence":"additional","affiliation":[{"name":"Advanced Cell Diagnostics, Inc Hayward California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cory T.","family":"Bernadt","sequence":"additional","affiliation":[{"name":"Department of Pathology and Immunology Washington University School of Medicine St. Louis 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