{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T18:12:09Z","timestamp":1776103929236,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,1,14]],"date-time":"2016-01-14T00:00:00Z","timestamp":1452729600000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>In pleural effusion cytology, the distinction of malignant mesothelioma (MM) from reactive mesothelial cells (RMCs) may be challenging, even with the aid of immunocytochemistry or fluorescence in situ hybridization. It has been demonstrated that several microRNAs (miRNAs) are useful for this purpose in cell lines and histologic samples. In the current study, the authors evaluated the utility of an miRNA\u2010based classifier as a complement to cytology.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>Quantitative reverse transcriptase\u2010polymerase chain reaction analysis of 15 miRNAs was performed in mesothelial (MET\u20105A) and MM (H28 and H2052) cell lines. Significant miRNAs were validated in 51\u2009MM and 40 nonneoplastic pleural histologic samples and then were tested in 29\u2009MM and 24 RMC cytologic specimens. The performance of individual and combined miRNAs was assessed for their ability to differentiate between MM and RMCs.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>MiRNA\u201019a (MiR\u201019a), miR\u201019b, miR\u201021, miR\u201025, and miR\u2010126 were differentially expressed in cell lines and histologic samples. MiR\u2010126 was down\u2010regulated in MM surgical specimens compared with nonneoplastic specimens, whereas all of the other miRNAs were overexpressed. In cytologic specimens, all miRNAs except miR\u201025 were confirmed, exhibiting a sensitivity or a specificity higher than the threshold of 0.80, as recommended by the International Mesothelioma Interest Group. The best classifier resulted from the combination of miR\u201021 and miR\u2010126, which achieved 0.86 sensitivity and 0.87 specificity.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>Subject to validation of the current results in further larger studies, miR\u201021 and miR\u2010126 profiling could be an effective and reliable tool for the diagnosis of MM in pleural effusions complementary to cytology evaluation. <jats:bold><jats:italic>Cancer (Cancer Cytopathol)<\/jats:italic> 2016;124:28\u201036.<\/jats:bold> \u00a9 <jats:italic>2016 American Cancer Society<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncy.21646","type":"journal-article","created":{"date-parts":[[2016,1,14]],"date-time":"2016-01-14T20:17:20Z","timestamp":1452802640000},"page":"28-37","source":"Crossref","is-referenced-by-count":37,"title":["Young investigator challenge: MicroRNA\u201021\/MicroRNA\u2010126 profiling as a novel tool for the diagnosis of malignant mesothelioma in pleural effusion cytology"],"prefix":"10.1002","volume":"124","author":[{"given":"Rocco","family":"Cappellesso","sequence":"first","affiliation":[{"name":"Department of Medicine, Surgical Pathology and Cytopathology Unit University of Padua Padua Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorenzo","family":"Nicol\u00e8","sequence":"additional","affiliation":[{"name":"Department of Medicine, Surgical Pathology and Cytopathology Unit University of Padua Padua Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brasilina","family":"Caroccia","sequence":"additional","affiliation":[{"name":"Department of Medicine, Surgical Pathology and Cytopathology Unit University of Padua Padua Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincenza","family":"Guzzardo","sequence":"additional","affiliation":[{"name":"Department of Medicine, Surgical Pathology and Cytopathology Unit University of Padua Padua Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Ventura","sequence":"additional","affiliation":[{"name":"Department of Statistical Sciences University of Padua Padua Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matteo","family":"Fassan","sequence":"additional","affiliation":[{"name":"Department of Medicine, Surgical Pathology and Cytopathology Unit University of Padua Padua Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ambrogio","family":"Fassina","sequence":"additional","affiliation":[{"name":"Department of Medicine, Surgical Pathology and Cytopathology Unit University of Padua Padua Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,1,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra050152"},{"key":"e_1_2_7_3_1","volume-title":"WHO Classification of Tumours of the Lung, Pleura, Thymus 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