{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T19:43:55Z","timestamp":1791229435894,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,12,18]],"date-time":"2015-12-18T00:00:00Z","timestamp":1450396800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Tissue specimens are typically considered optimal for molecular testing; however, in the current era of personalized medicine, cytopathology specimens are increasingly recognized as potential sources for molecular testing. This is often accomplished by using cell block specimens and\/or fine\u2010needle aspiration (FNA) smear preparations. In this study, the authors investigated the feasibility, performance, and quality of \u201cresidual\u201d FNA rinse and body effusion fluids used for next\u2010generation sequencing (NGS).<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>Sequence data were generated from 17 malignancies in 16 patients from 13 FNA (10 lymph nodes, 1 lung, and 2 bone lesions) and 4 effusion (3 pleural and 1 pericardial) specimens. Malignancies included carcinomas (lung, breast, ovarian, and unknown primary), melanoma, and myeloma. Paired NGS testing was performed in 7 patients who had surgical biopsy or cell block specimens available. Routinely processed residual FNA rinse material and body fluids were used for DNA extraction and NGS (targeted gene panel).<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>NGS was successfully performed on all 17 specimens. A significant amount of DNA was obtained from the residual FNA rinse (176.3\u2009ng\/\u03bcL) compared with the paired cell block slides (10.6\u2009ng\/\u03bcL). Two of the 10 lung adenocarcinomas (20%) demonstrated epidermal growth factor receptor (<jats:italic>EGFR<\/jats:italic>) mutations, including 1 leucine\u2010to\u2010arginine substitution at codon 858 (L858R) in exon 21 and 1 codon 2235_2249 deletion (resulting in an in\u2010frame deletion of 5 amino acids from position 746 to 750 [glutamic acid, leucine, arginine, glutamic acid, and alanine]; E746_A750del) in exon 19. Three <jats:italic>KRAS<\/jats:italic> [Kirsten rat sarcoma viral oncogene homolog] mutations, 1 <jats:italic>BRAF<\/jats:italic> (v\u2010Raf murine sarcoma viral oncogene homolog B1) mutation, and 1 <jats:italic>NRAS<\/jats:italic> (neuroblastoma RAS viral oncogene homolog) mutation were identified in the remaining lung adenocarcinomas. Patients who underwent paired testing demonstrated 100% concordant mutations.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>Targeted NGS can be performed on residual FNA rinse and body fluid specimens. This approach is particularly important when a paucicellular cell block or biopsy specimen is encountered. <jats:bold><jats:italic>Cancer Cytopathol<\/jats:italic> 2016;124:324\u201329<\/jats:bold>. \u00a9 <jats:italic>2015 American Cancer Society<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncy.21666","type":"journal-article","created":{"date-parts":[[2015,12,18]],"date-time":"2015-12-18T21:45:00Z","timestamp":1450475100000},"page":"324-329","source":"Crossref","is-referenced-by-count":72,"title":["Using \u201cresidual\u201d FNA rinse and body fluid specimens for next\u2010generation sequencing: An institutional experience"],"prefix":"10.1002","volume":"124","author":[{"given":"Shuanzeng","family":"Wei","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Center for Personalized Diagnostics Hospital of the University of Pennsylvania Philadelphia Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Lieberman","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Center for Personalized Diagnostics Hospital of the University of Pennsylvania Philadelphia Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer J. D.","family":"Morrissette","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Center for Personalized Diagnostics Hospital of the University of Pennsylvania Philadelphia Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zubair W.","family":"Baloch","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Center for Personalized Diagnostics Hospital of the University of Pennsylvania Philadelphia Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David B.","family":"Roth","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Center for Personalized Diagnostics Hospital of the University of Pennsylvania Philadelphia Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cindy","family":"McGrath","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Center for Personalized Diagnostics Hospital of the University of Pennsylvania Philadelphia Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,12,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2014-0250-CP"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.12.5463"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/gim.2013.92"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-14-0327"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPU3J7FGAYQBRL"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/SLA.1156"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCP40XETVYAMJPY"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21464"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2013.03.001"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65461-2"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2013.05.004"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64472-0"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21476"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2013.122"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21361"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.29038"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2013-2292"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2012.11.006"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.20151"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2012-0561-CP"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-012-1219-x"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0083607"}],"container-title":["Cancer Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcncy.21666","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/acsjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cncy.21666","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T20:44:08Z","timestamp":1760301848000},"score":1,"resource":{"primary":{"URL":"https:\/\/acsjournals.onlinelibrary.wiley.com\/doi\/10.1002\/cncy.21666"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,12,18]]},"references-count":22,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2016,5]]}},"alternative-id":["10.1002\/cncy.21666"],"URL":"https:\/\/doi.org\/10.1002\/cncy.21666","archive":["Portico"],"relation":{},"ISSN":["1934-662X","1934-6638"],"issn-type":[{"value":"1934-662X","type":"print"},{"value":"1934-6638","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,12,18]]}}}