{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:31:30Z","timestamp":1790836290211,"version":"4.1.0"},"reference-count":17,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2016,5,2]],"date-time":"2016-05-02T00:00:00Z","timestamp":1462147200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>The liquid\u2010based cytology Papanicolaou (Pap) test has gradually replaced the conventional Pap test, as it seems to be more efficient, in part because it uses computer assistance. However, Pap test diagnoses are still subjective and are cost\u2010consuming or time\u2010consuming. The authors describe a new digital holographic microscopy instrument and accompanying software. This technology provides an instantaneous, 3\u2010dimensional image reconstruction of cells directly from the uterine cervical sample vial.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>Analyses were performed using the proprietary digital holographic microscopy (DHM) instrument, computer, and software. Residual materials from 3 randomly chosen laboratories were analyzed and subjected to DHM, and the sensitivity and specificity of DHM were calculated for the detection neoplasia.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>In 41 specimens that yielded normal Pap test results, 1333 cells were analyzed using DHM; and, in 30 specimens that yielded abnormal Pap test results, 494 cells were analyzed using DHM. Available histologic correlation was as follows: 4 histologic samples were diagnosed as grade 1 cervical intraepithelial neoplasia (corresponding to 2 cytologic diagnoses of low\u2010grade squamous intraepithelial lesion and 2 cytologic diagnoses of atypical squamous cells of undetermined significance), 4 histologic samples were diagnosed as grade 3 cervical intraepithelial neoplasia (corresponding to 2 cytologic diagnoses of low\u2010grade squamous intraepithelial lesion and 2 cytologic diagnosis of atypical squamous cells, cannot rule out high\u2010grade squamous intraepithelial lesion), and 11 lesions were diagnosed as benign. Receiver operating characteristic curve analysis indicated that the best criteria were the nuclear\u2010to\u2010cytoplasmic ratio (area under the curve, 0.88; 95% confidence interval, 0.68\u20101.00) and the greatest nuclear dimension (area under the curve, 0.85; 95% confidence interval, 0.66\u20101.00).<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>This preliminary study demonstrated for the first time that the DHM technique is suitable for the processing of gynecologic cervical samples. Nevertheless, DHM criteria and parameters could be better defined. Hopefully, holographic analysis will be performed automatically and will provide an instantaneous, cost\u2010effective diagnosis from a closed vial with the preservation of all cellular material. <jats:bold><jats:italic>Cancer Cytopathol<\/jats:italic> 2016;124:573\u201080<\/jats:bold>. \u00a9 <jats:italic>2016 American Cancer Society<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncy.21727","type":"journal-article","created":{"date-parts":[[2016,5,2]],"date-time":"2016-05-02T20:02:26Z","timestamp":1462219346000},"page":"573-580","source":"Crossref","is-referenced-by-count":32,"title":["Digital holographic microscopy as screening tool for cervical cancer preliminary study"],"prefix":"10.1002","volume":"124","author":[{"given":"Nazim","family":"Benzerdjeb","sequence":"first","affiliation":[{"name":"Department of Pathology University Hospital of Amiens Amiens France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Garbar","sequence":"additional","affiliation":[{"name":"Department of Biopathology Institute of Cancerology Jean Godinot Reims France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Camparo","sequence":"additional","affiliation":[{"name":"Center of Pathology Amiens France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henri","family":"Sevestre","sequence":"additional","affiliation":[{"name":"Department of Pathology University Hospital of Amiens Amiens France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,5,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1364\/OL.30.000468"},{"key":"e_1_2_8_3_1","first-page":"6244","article-title":"Digital holographic microscopy applied to life sciences","volume":"2007","author":"Depeursinge C","year":"2007","journal-title":"Conf Proc IEEE Eng Med Biol Soc."},{"key":"e_1_2_8_4_1","first-page":"2339","article-title":"Proving tumour cells by acute nutritional\/energy deprivation as a survival threat: a task for microscopy","volume":"29","author":"Janeckova H","year":"2009","journal-title":"Anticancer Res."},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1364\/AO.45.000864"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.2357174"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1364\/AO.44.003827"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2288-14-26"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/0962280215602040"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1086\/657321"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/s100908437"},{"key":"e_1_2_8_12_1","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1182\/blood.V46.3.369.369","article-title":"Erythrocyte volume distribution in normal and abnormal subjects","volume":"46","author":"Bessman JD","year":"1975","journal-title":"Blood."},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-011-9584-y"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2ib20227a"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1364\/OE.17.012031"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.21541"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.21439"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.285.11.1500"}],"container-title":["Cancer Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcncy.21727","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/acsjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cncy.21727","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T09:58:01Z","timestamp":1760263081000},"score":1,"resource":{"primary":{"URL":"https:\/\/acsjournals.onlinelibrary.wiley.com\/doi\/10.1002\/cncy.21727"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,5,2]]},"references-count":17,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2016,8]]}},"alternative-id":["10.1002\/cncy.21727"],"URL":"https:\/\/doi.org\/10.1002\/cncy.21727","archive":["Portico"],"relation":{},"ISSN":["1934-662X","1934-6638"],"issn-type":[{"value":"1934-662X","type":"print"},{"value":"1934-6638","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,5,2]]}}}