{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T23:49:59Z","timestamp":1773272999844,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2016,11,28]],"date-time":"2016-11-28T00:00:00Z","timestamp":1480291200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Molecular testing provides an important ancillary study for thyroid nodules with indeterminate cytology. The nomenclature shift to \u201cnoninvasive follicular thyroid neoplasm with papillary\u2010like nuclear features\u201d (NIFTP) will impact the performance of molecular tests. For the current study, the authors reviewed the performance of the Afirma gene\u2010expression classifier (GEC) and the University of Pittsburgh Medical Center (UPMC) targeted mutation panel tests in thyroid nodules that were subsequently diagnosed as NIFTP on surgical resection.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>In total, 302 nodules were sent for molecular testing between June 2012 and June 2016. These cases were retrospectively reviewed to identify patients who underwent subsequent surgical resection and were diagnosed with follicular variant of papillary thyroid carcinoma (FVPTC). Twenty\u2010five nodules that were diagnosed as FVPTC met the initial inclusion criteria. These cases were reviewed using strict criteria to identify NIFTP.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Eight cases met criteria for NIFTP, and 4 NIFTPs underwent Afirma testing. Cytology diagnoses were all Bethesda category III, with 3 diagnosed as atypia of undetermined significance (AUS) and 1 diagnosed as follicular lesion of undetermined significance (FLUS). All of these nodules were identified as \u201csuspicious\u201d using GEC. Four NIFTPs underwent testing at UPMC, all using ThyroSeq V2. The cytology diagnoses for these nodules also were category III, with the exception of 1 nodule that was category IV, suspicious for follicular neoplasm. All NIFTPs were positive for mutations, all of which were <jats:italic>RAS<\/jats:italic> mutations (<jats:italic>NRAS, KRAS<\/jats:italic>). One patient who had a nodule classified as NIFTP had metastatic carcinoma identified in a lymph node. Another who had a 6\u2010cm tumor had coexisting <jats:italic>NRAS<\/jats:italic> and <jats:italic>TERT<\/jats:italic> mutations.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>The current results indicate that NIFTP is a rare tumor if defined by strict criteria, that both the GEC and UPMC methods indicate abnormalities in NIFTP, and further independent study will be needed to better characterize the molecular and clinical characteristics of NIFTP. <jats:bold><jats:italic>Cancer Cytopathol<\/jats:italic> 2016;124:893\u2013900<\/jats:bold>. \u00a9 <jats:italic>2016 American Cancer Society<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncy.21802","type":"journal-article","created":{"date-parts":[[2016,11,28]],"date-time":"2016-11-28T10:20:52Z","timestamp":1480328452000},"page":"893-900","source":"Crossref","is-referenced-by-count":55,"title":["Young Investigator Challenge: Molecular testing in noninvasive follicular thyroid neoplasm with papillary\u2010like nuclear features"],"prefix":"10.1002","volume":"124","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3069-3130","authenticated-orcid":false,"given":"Xiaoyin \u201cSara\u201d","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Pathology Duke University Hospital Durham North Carolina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grant P.","family":"Harrison","sequence":"additional","affiliation":[{"name":"Department of Pathology Duke University Hospital Durham North Carolina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael B.","family":"Datto","sequence":"additional","affiliation":[{"name":"Department of Pathology Duke University Hospital Durham North Carolina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,11,28]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1089\/thy.2009.0274"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1203208"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2013-2292"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1089\/thy.2015.0020"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoncol.2016.0386"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21631"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/thy.2014.0612"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1089\/thy.2016.0280"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1089\/thy.2015.0644"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1089\/thy.2016.0244"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00268-005-0356-7"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.22138"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21744"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1208822"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.09.050"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12916-015-0419-z"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2016.65"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2014.59.4614"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.7811"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/cen.12999"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.29934"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21624"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21749"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21455"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2013-3584"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2013-2482"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1159\/000446797"},{"key":"e_1_2_8_29_1","first-page":"3996","article-title":"Wide inter\u2010institutional variation in performance of a molecular classifier for indeterminate thyroid nodules. 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