{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:38:21Z","timestamp":1786977501178,"version":"build-2736575974"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,12,5]],"date-time":"2016-12-05T00:00:00Z","timestamp":1480896000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Folke Hermansen Fund"},{"name":"Roche Diagnostics GmbH (Mannheim, Germany)"},{"name":"CINtec PLUS kits for the p16\/Ki-67"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>More accurate biomarkers in cervical cytology screening could reduce the number of women unnecessarily referred for biopsy. This study investigated the ability of p16\/Ki\u201067 dual staining to predict high\u2010grade cervical intraepithelial neoplasia (CIN) in human papillomavirus (HPV)\u2013positive women from the Norwegian Cervical Cancer Screening Program.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>Automated p16\/Ki\u201067 dual staining was performed on liquid\u2010based cytology samples from 266 women who were HPV\u2010positive at their secondary screening. At a mean of 184 days after p16\/Ki\u201067 staining, 201 women had a valid staining result and a conclusive follow\u2010up diagnosis (histological diagnosis or HPV\u2010negative diagnosis with normal cytology findings). The sensitivity and specificity for predicting the follow\u2010up diagnosis were compared for cytology, p16\/Ki\u201067 dual staining, and their combination.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Sixty\u2010seven percent of the study sample was p16\/Ki\u201067\u2013positive. The sensitivity of p16\/Ki\u201067 staining for predicting CIN\u20102\/3 was statistically significantly higher than the sensitivity of cytology (0.88 vs 0.79;\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u2009.008), but this was not true for the prediction of CIN\u20103 (0.94 vs 0.88;\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u2009.23). The specificity of cytology for predicting CIN\u20103 was significantly higher than the specificity of p16\/Ki\u201067 staining (0.35 vs 0.28;\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u2009.002), but this was not true for CIN\u20102\/3 (0.35 vs 0.31;\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u2009.063). For predicting CIN\u20102\/3 and CIN\u20103, combination testing gave potentially better sensitivity (0.95 and 0.96, respectively) and better specificity (0.49 and 0.50, respectively).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS<\/jats:title>\n                    <jats:p>\n                      In a population of HPV\u2010positive women, p16\/Ki\u201067 dual staining was more sensitive but less specific than cytology for predicting high\u2010grade CIN. The advantage of using both tests in different combinations is the potential for increasing the specificity or sensitivity in comparison with both methods performed individually.\n                      <jats:bold>\n                        <jats:italic>Cancer Cytopathol<\/jats:italic>\n                        2017;125:283\u2013291.\n                      <\/jats:bold>\n                      \u00a9\n                      <jats:italic>2016 American Cancer Society.<\/jats:italic>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cncy.21807","type":"journal-article","created":{"date-parts":[[2016,12,5]],"date-time":"2016-12-05T12:28:59Z","timestamp":1480940939000},"page":"283-291","source":"Crossref","is-referenced-by-count":14,"title":["Clinical value of fully automated p16\/Ki\u201067 dual staining in the triage of HPV\u2010positive women in the Norwegian Cervical Cancer Screening Program"],"prefix":"10.1002","volume":"125","author":[{"given":"Irene T.","family":"Ovestad","sequence":"first","affiliation":[{"name":"Department of Pathology Stavanger University Hospital Stavanger Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingvild","family":"Dalen","sequence":"additional","affiliation":[{"name":"Section of Biostatistics, Department of Research, Stavanger University Hospital Stavanger Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth","family":"Hansen","sequence":"additional","affiliation":[{"name":"Department of Pathology Stavanger University Hospital Stavanger Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janne L. D.","family":"Loge","sequence":"additional","affiliation":[{"name":"Department of Pathology Stavanger University Hospital Stavanger Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Britt Mona","family":"Dybdahl","sequence":"additional","affiliation":[{"name":"Department of Pathology Stavanger University Hospital Stavanger Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marius B.","family":"Dirdal","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine Faculty of Medicine and Dentistry, University of Bergen Bergen Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pia","family":"Moltu","sequence":"additional","affiliation":[{"name":"Department of Pathology Stavanger University Hospital Stavanger Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jannicke M.","family":"Berland","sequence":"additional","affiliation":[{"name":"Department of Pathology Stavanger University Hospital Stavanger Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,12,5]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-04-0569"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(199909)189:1<12::AID-PATH431>3.0.CO;2-F"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-08-4192"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3109\/0284186X.2014.932433"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2012.489"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2014.11.076"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.c1804"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctrv.2008.10.005"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2005.027839"},{"key":"e_1_2_9_11_1","first-page":"2979","article-title":"P16 and Ki\u201067 expression improves the diagnostic accuracy of cervical lesions but not predict persistent high risk human papillomavirus infection with CIN1","volume":"8","author":"Zhong P","year":"2015","journal-title":"Int J Clin Exp Pathol."},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e3181e8b2c4"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPXSVCDZ3D5MZM"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2011.02.033"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.20140"},{"key":"e_1_2_9_16_1","unstructured":"Norwegian Cervical Cancer Screening Program.http:\/\/www.kreftregisteret.no\/en\/Cancer-prevention\/Cervical-Cancer-Screening-Programme\/. 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